báo cáo khoa học: " Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility" potx

... show that native AtTOR binds in vitro to recombinant ScFKBP12 in the presence of rapamycin and that expression of ScFKBP12 in transgenic plants results in a partial and rapamycin- dependent arrest ... [13]. Rapamycin leads to the formation of a ternary complex by binding simultaneously to the FRB [FKP12 and Rapamycin Binding] domain of TOR and to the S...

Ngày tải lên: 12/08/2014, 05:20

... cooperative and farmers. Training of one NLU staff member in Australia and visits of project leader to rice research institutes in Thailand and Philippines will take place in October 2006. ... of milling losses in two provinces. The milling loss data were collected directly from three milling plants in each province (Kien Giang and Tien Giang). Since the work is bei...

Ngày tải lên: 21/06/2014, 06:20

... subgroups [18,30,31], including the bHLH-interaction and calmodulin-binding motifs in the DNA-binding domain [28,29], and the repression domain pdLNLD/ELxiG/S in the C-terminus [7]. In our study, the ... sequences belonging to each phylogenetic clade. The R2R3-MYBs are specific to plants and are subdivided into three major groups according to their binding affini- ties [19...

Ngày tải lên: 12/08/2014, 05:20

... mitochon- drial protein suspended in 0.6 m sorbitol, 10 mm Tris ⁄ HCl (pH 7.5) and 1 mm EDTA were maintained at 65 mV by incubation for 2 min with KCl (12.4 mm), valinomycin (0.1 lgÆmL )1 ) and ... branched path to the iron–sulfur protein and to cytochrome b L [4]. Biosynthesis of coenzyme Q in eukaryotes occurs in mitochondria. In Saccharomyces cerevisiae, the ben- zene...

Ngày tải lên: 06/03/2014, 22:21

... covalently linked to inositol in yeast [29,30], we examined the ability of Ybr004cp-depleted cells to incor- porate [ 3 H]inositol into proteins. The ybr004cD-pGAL- YBR004c strain was grown and labeled ... walls and abnormal morphology, are unable to incorporate [ 3 H]inositol into proteins, and accumulate a GPI intermedi- ate having a single mannose that is likely modified with...

Ngày tải lên: 07/03/2014, 16:20

... EcoRI and HindIII, and inserted into a similarly cut YEp356 vector [41], resulting in plasmid pHPR164. The PEX5 promoter was obtained from plasmid YCpPEX5 [19] as a HindIII–NheI fragment containing 194 ... also investigated whether Pip2p–Oaf1p is important for regulating genes encoding peroxins involved in protein import into the peroxisomal matrix. Pip2p–Oaf1p was able to bind effici...

Ngày tải lên: 17/03/2014, 03:20

... carried out according to the manufacturer’s protocol. The expression vector (pPICZaA-ScOCH1, Fig. 1A) was linearized by PmeI (New England Biolabs) and used to transform P. pastoris by the electroporation ... tri-saccharide and its derivative, in which the reducing terminus was either free and modified with b-linked fluorine to mimic the lower arm of native acceptors, respectiv...

Ngày tải lên: 23/03/2014, 10:20

... C-terminal domain of approximately 90 residues that protrudes into the matrix and functions as a ribosome binding domain (RBD) [37,38]. This domain is not conserved in YidC and Cox18. To study ... protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Esc- herichia coli, YidC plays a key role in the integration and ass...

Ngày tải lên: 30/03/2014, 03:20

... apparatus and its regulatory components were located. This included myosin, actin, t roponin and tr opomyosin. Other a bundant proteins, s uch as a lbumin, desmin, aldolase, carbonic anhydrase and ... for silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling or immunoblotting. T he following running conditions were used: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min a t...

Ngày tải lên: 19/02/2014, 16:20

... NAIST. (In Japanese). Andrew McCallum, Kamal Nigam, Jason Rennie, and Kristie Seymore. 1999. A machine learning ap- proach to building domain-specific search engines. In Proceedings of the 16th International ... Discovery and Data Mining, pages 420–431. Makoto Iwayama and Takenobu Tokunaga. 1994. A probabilistic model for text categorization: Based on a single random variable with m...

Ngày tải lên: 20/02/2014, 18:20