báo cáo khoa học: " Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers" ppt

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... con-taining DNA spots (5 lg each) corresponding to inter-nal fragments of ADC or cruzipain genes. The latter(used as a control) is a housekeeping gene that encodesthe main cysteine proteinase of ... requiringplasmid duplication during integration, the particularstructure of plasmid pADC-8 and ⁄ or the involvement of putative intermediate forms of the transformingplasmid might explain the ... to a-amanitin of the transcription of argininedecarboxylase (ADC) and cruzipain genes in transformed Trypano-soma cruzi. Permeable parasites were preincubated at 0 °C for5 min in the absence...

Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt

... overall arginine levels. The levels of ornithine, citrulline and arginineremained low, at between 0.01 and 0.04 lmolÆg)1freshweight (Fig. 3E). However, arginine accumulated up to70-fold and 80-fold ... composed of 33 exons coding fora protein of 165 kDa, containing a class II (purF)-typeglutaminase domain and short regions for binding toFMN and iron sulfur center. GLT is composed of 20Amino ... (ORN), citrulline (CIT) and arginine (ARG) contents in leaves of Arabidopsis plants cultured with 5 mM nitrate in air. (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis...

Báo cáo khoa học: "Detection of Bartonella species from ticks, mites and small mammals in Korea" pps

... class="bi x0 y2 w1 h 3" alt ="" ...

Báo cáo khoa học: " Prevalence of feline herpesvirus 1, feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter" ppt

... Hotaling S, Schiebel A, Ashbaugh SE, Roberts SM, Collins JK. Comparison of PCR, virus iso-lation, and indirect fluorescent antibody staining in the de-tection of naturally occurring feline ... through decreasing the extent of crowding. Although vaccination with FHV-1 and FCV cannot lead to prevention of infection and viral shedding, this may reduce the overall severity of disease [16]. ... many shelter cats have been infected with FHV-1 and they remain subclinical carriers after recovery. At least 80% of infected cats remain latently infected and 29% of them shed virus spontaneously...

báo cáo khoa học: "Localization of DIR1 at the tissue, cellular and subcellular levels during Systemic Acquired Resistance in Arabidopsis using DIR1:GUS and DIR1:EGFP reporters" ppsx

... Examina-tion of these lines indicated that the DIR1 promoterregion initiated expression of GUS and DIR1:GUS in seedlings, roots and floral tissues and in all living cellsincluding the veins and mesophyl ... family. In vitro, DIR1 can bind two monoacy-lated phospholipids and contains two proline-rich SH3domains. SH3 domains participat e in protein-proteininteractions in numerous proteins [23]. ... observed reduction in GUS activity observed in Figure 1A. Intense staining occurred in the midvein and secondary veins in mock-inoculated or untreated leaves of both the DIR1pro:GUS-11 and DIR1pro:DIR1-GUS-29...

báo cáo khoa học: " Years of life lost to prison: racial and gender gradients in the United States of America" pps

... table value of the total remaining years of life and the corresponding life expectancy in each state for eachage group. Total life expectancy at birth or any other agegroup by race and gender ... generally and drug policy in particular.Competing interestsThe author(s) declare that they have no competing inter-ests.Authors' contributionsRSH, SB, ED, and SAS initiated the study. RSH and ... the differences in the number of years of lifelost to imprisonment in the United States population bygender and race.MethodsThe number of years of life lost to imprisonment in theUnited States...

Báo cáo sinh học: " Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly" pptx

... mMglutamine (Invitrogen), and 10 mM gentamicin sulfate(Invitrogen) at 37°C in a 95% humidified atmospherecontaining 5% CO2. For infection of WR, BSC-40 cellswere maintained in infection ... by stain-ing with Coomassie R-250 and de-staining until proteinbands could be easily visualized. Protein bands of interestwere excised in as small of piece of gel as possible. The gelslices ... virus, indicating distinct regulation/func-tion of VV proteolysis [11]. In addition, A12L containstwo other AG/K sites in the internal region and C-termi-nus of A12L open reading frame (ORF), of...

Báo cáo hóa học: " Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly" ppt

... For infection of WR, BSC-40 cellswere maintained in infection media (EMEM) supple-mented with 5% FCS, 2 mM glutamine, and 10 mM gen-tamicin sulfate and were infected at a multiplicity of infection ... virus, indicating distinct regulation/func-tion of VV proteolysis [11]. In addition, A12L containstwo other AG/K sites in the internal region and C-termi-nus of A12L open reading frame (ORF), of ... by stain-ing with Coomassie R-250 and de-staining until proteinbands could be easily visualized. Protein bands of interestwere excised in as small of piece of gel as possible. The gelslices...

Báo cáo khoa học " Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice " pptx

... the CY-induced atrophy of immune organs. In line with the usage of Cordyceps in China, Chinese medicinesare strongly recommended for the ageing population ... Feib,Ying Lib, Yuan liang Hua,∗, Fei Qun Xueb,∗∗aInstitute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural ... An increas-ing amount of evidence indicates that many kinds of polysaccha-rides have potential and potent capabilities of preventing oxidativedamage in ...

báo cáo khoa học: " Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers" ppt

... is the main dietary precursor of vitamin A. Potato tubers contain low levels of carotenoids, composed mainly of the xanthophylls lutein (in the beta-epsilon branch) and violaxanthin (in the beta-beta ... β-hydroxylation,zeaxanthin, decreased 2- to 8-fold, while violaxanthinincreased significantly in two out of four lines and neox-anthin increased significantly in one line. The final prod-uct of the competing ... purposes)BMC Plant BiologyOpen AccessResearch article Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubersGianfranco Diretto1, Ralf Welsch2,...

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