báo cáo khoa học: " Kinesin-5 motors are required for organization of spindle microtubules in Silvetia compressa zygotes" doc

... maintenance of spindle pole sepa- ration [31]. Kinesin-5 proteins act in concert with other spindle associated proteins to maintain proper spindle length. For example, increasing the levels of ... chromosomes, thereby becoming a supernumerary spindle pole. In this model, Kinesin-5 motors must organize and maintain the integ- rity of spindle poles. Kinesin-5 membe...

Ngày tải lên: 12/08/2014, 05:20

... incubating 50 lg of protein in 30 lLof50m M potassium phosphate buffer, pH 7.0, containing 50 m M KCl, protease and esterase inhibitors, and [ 3 H]AcCoA (0.2 lCi, 8.3 l M )at 37 °C for 15 min. ... cloning, remaining elusive despite the efforts of a number of groups. Nevertheless, the information reported here adds consider- ably to our understanding of sialate O-acetylation regul...

Ngày tải lên: 07/03/2014, 14:20

... [ 3 H]Daunomycin binding to the ABCG2 R482G isoform was examined in the nucleotide-bound and post-hydrolytic conformations. Binding of [ 3 H]daunomycin was displaced by ATP analogues, indicating transition ... FEBS [ 3 H]daunomycin, in a total volume of 100 lL in polypro- pylene test tubes for 120 min to ensure attainment of bind- ing equilibrium. The membranes, [ 3 H]daunomycin...

Ngày tải lên: 18/02/2014, 18:20

... similar structures in an isolated soluble form and in a crystal- line form, integrated within the full length gpE [24,25]. Contribution of the CDR3 loops to the energy of interaction The CDR3 loops of mAb4E11 ... Unraveling hot spots in binding interfaces: progress and challenges. Curr Opin Struct Biol 12, 14–20. 33 MacArthur MW & Thornton JM (1991) In uence of proline resi...

Ngày tải lên: 19/02/2014, 07:20

... sliding window techniques (Maarek et al., 1991). The window is a slide from the first word of the document to the last in the size of the window (60 words) and the interval of each window ... number of contexts that contain W 1 , is 1. 4) Assigning remaining contexts to a category We decided a similarity value of each remaining context for each category using the followi...

Ngày tải lên: 20/02/2014, 16:20

... reduce the overhead of parallelization. Note that copying in the procedures makes it possible to support non-determinism in NLP systems. For instance, during parsing, interme- diate parse ... i~ii iiii ~!~iiiiiiiiii: ~ Shared Heap Area PSTFS Figure 3: Inside of the PSTFS perform operations on the same data. This can be done by introducing copying of TFSs, which does no...

Ngày tải lên: 20/02/2014, 18:20

... underlying the recognition of protein quality acts through the sensing of free circulating amino acids resulting from the intestinal digestion of proteins [4,5]. Another example of the detection of ... is directly involved in the regulation of amino acid and protein metabolism, as many of the genes involved in these processes are not regulated in GCN2) ⁄ ) cells. These r...

Ngày tải lên: 07/03/2014, 03:20

... how to control quality of mass concrete with reducing internal restraining stress through finite analysis. As a result, a thermal crack index is over 1.0 and a curing time of mass-section foundation ... concrete from initial time of hardening. Especially, a volume change in surface of mass-section element is almost same by cooling from low temperature in the air while inside o...

Ngày tải lên: 07/03/2014, 03:20

... analyzed by western blotting using anti- bodies to RasGRF1 and a-tubulin. A B Fig. 5. DHPH2 domain of RasGRF1 binds directly to microtubules. In vitro binding of recombinant regions of RasGRF1 to pure ... region responsible for the interaction of the entire RasGRF1 molecule with microtubules. Pure microtubules were incubated for 20 min with RasGRF1-containing cell extracts (...

Ngày tải lên: 07/03/2014, 12:20

... the side chain of Val216 interfere with binding of a 4-linked glucose. The CG1 of Val216 is too close to the O3¢ of maltose. On the other hand, both isomaltose a nd maltose can bind to th e V216T mutant ... nucleophile in consensus region II must b e involved in the recognition of a-glucosidic linkages. In conclusion, this work was successful in identifying the region and re...

Ngày tải lên: 07/03/2014, 16:20