Báo cáo khoa học: "Enhanced anti-HCV activity of interferon alpha 17 subtype" ppt

Báo cáo khoa học: "Impaired antiviral activity of interferon alpha against hepatitis C virus 2a in Huh-7 cells with a defective Jak-Stat pathway" pdf

... homology of the 5’ and 3’ UTRsamong all genotypes of HCV, the efficiency of RNAgenome replication of different HCV strains in the cellculture varies significantly [13]. Some strains of HCVwith ... 7:36http://www.virologyj.com/content/7/1/36Page 11 of 16expression of this JFH1-GFP chimera was seen in Huh-7.5 cells 24 h after transfection. The expression of GFPin the transfected cells is an indication of ac tive replica-tion of the ... (between 417 and 418 amino acids of NS5Aprotein). Changes in the nucleotide and amino acid sequences of NS5A gene of HCV-GFP chimera clone are shown. GFP was also inserted atthe similar location of...

Báo cáo khoa học: "Enhanced anti-HCV activity of interferon alpha 17 subtype" ppt

... http://www.virologyj.com/content/6/1/70Page 3 of 10(page number not for citation purposes)Determination of anti-HCV efficacyHuh7 cells were infected with JFH-1 at a multiplicity of infection (MOI) of 0.001. After three weeks of infection,chronically ... a net charge of 0 andIFN α8 and IFN 17 both have a net charge of 3. Thereplacement of IFN α2's tail by an IFN α8 tail increasedthe binding of IFN α2 to IFNAR2 by a factor of 20 andtranslated ... of four independent experiments.0,0010,010,11IFNneg 1 6 16 4b 14 2a 5 21 10 8 7 17 IFN alpha subtypes (260 pg/mL)Inhibition (log)***110100100010000IFNneg12a7 817 interferon alpha...

Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

... from the inset data. Plots of percent-age control activity versus concentration of inhibitor wereused to determine the concentrations that inhibited 50% of protease activity. The enzymes evaluated, ... theprocessing of apicoplast protein leader sequence.Inhibition of any of these activities could contribute toMA interference in the maturation of the parasite.Polypharmacology of MAThe observed ... DiscussionInhibition of proteases by MAIt has been previously proposed that MA may inhibitthe activity of proteases of T. gondii [14] and HIV[12,31]. To ascertain the inhibition range of MA onthe...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... hydrogenase-2 activity becauseassembly of the enzyme is disrupted; however, co-expression of active torD restores the Tat proofreading of this enzyme and so rescues hydrogenase-2 activity in ... assay measuring the effect of compe-tition of a ten-fold excess of XMP on the GTPaseactivities of the native protein and the TorDD68Nvari-ant. The GTPase activity of TorDD68Nwas found to ... elucidated, there are aspects of this activity that are mirrored in canonical nucleo-tide hydrolases. For example, the GTPase activity of TorD is dependent upon the presence of magnesiumions and this...

Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx

... recognition of p NPP by the pNPP site at t he rear su rface of the N -domainis due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398. The hydrophobicity of the ... optimized.ResultsEffects of truncation of the cytoplasmic H4-H5loop of thea subunit of Na+/K+ATPase on TNP-ATP binding andp-nitrophenylphosphatase activity Molecular modeling of the H4-H5loop ... volume of 40 mMTris/HCl, pH 7.4. Binding of FITC was determined as themolar ratio of FITC bound per H4-H5loop or per the asubunit of Na+/K+ATPase [17] . K+activated phospha-tase activity...

Báo cáo khoa học: Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase ppt

... tochanges in concentration of the more toxic isomer of chiral OPs and reﬂects the rate of detoxiﬁcation, ratherthan degradation, of cyclosarin, IMP-pNP and soman.In the case of symmetric OPs such ... of PON1 [18]. However, Yeung et al. [17] have shown that the paraoxonase activity of H115W PON1 is even higher than that of the wild-Fig. 9. Time-course of IMP-pNP degradation and detoxiﬁcation ... less toxic optical isomers of cyclosarin[30] and those of p-nitrophenol analogs of sarin andsoman [25]. Therefore, it was of interest to examinethe stereoselectivity of cyclosarin and soman hydro-lysis...

Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc

... Antibacterial activity of LK peptides (Eur. J. Biochem. 270) 2 217 The antibiotic activity of cationic linear amphipathic peptides:lessons from the action of leucine/lysine copolymers on bacteria of the ... [LK15(3.6)] the activity of which was similar to that of melittin, a beevenom peptide known as one of the most efﬁcient naturalpeptides in killing bacteria [8,39–41].Lengthening peptides of the alpha- amphi ... action of these peptides comprises four main steps:step 1, interfacial partitioning and exofacial accumulation of Fig. 5. Graphical illustration of the eﬀects on antibacterial activity of thelength...

Báo cáo khoa học: Ion channel activity of brain abundant protein BASP1 in planar lipid bilayers pptx

... Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology of RAS, St Petersburg, Russia2 Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute of RAS, ... presence of liposomes composed of anionic lipids [35]. The forma-tion of an ion-conducting channel probably proceedsthrough the association of several BASP1 monomers onthe surface of the anionic ... %) bilayers, regardless of the proteinconcentration and method of incorporation (addition of soluble GAP-43 or GAP-43-containing liposomes).Multiple conductance states of BASP1 channelsBesides...

Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot

... enzymatic activity of the nuclear matrix-localized SRPK1 is repressed. These results suggest a new role for SAFBproteins as regulators of SRPK activity and underline the importance of theassembly of ... signiﬁcance of enzyme–substrate contacts forcatalysis. This study highlights the importance of theinteraction of a phosphoserine of the RS domain of the substrate with the catalytic region of the ... phosphorylating activity of SRPK1a.FLAG–SRPK1 activity is inhibited in vitro bySAFB1 and SAFB2We next asked whether the inhibition exerted bySAFB1 on SRPK1a activity is because of its inter-action...

Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc

... Photon Source, Argon-ne National Laboratory. Use of the Advanced PhotonSource was supported by the U. S. Department of Energy, Of ce of Science, Of ce of Basic Energy Sci-ences, under Contract No. ... black.(C) Stereoview of the alignment of theactive sites of AfRio1 (green) and AfRio2(orange; PDB code 1ZAO). (D) Stereoview of the alignment of the active sites andbound nuceotide of AfRio1 (green) ... the position of the substrate peptide.DiscussionDetermination of the structure of AfRio1 and its com-parison with the structure of AfRio2 has allowed us todeﬁne the minimal extent of a RIO domain....

Xem thêm