Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot

... isolates of DEV will also be of importance in order to assess the possible role of the UL31 protein in viral pathogenesis. These properties of the UL31 protein provide a prerequisite for further ... analysis of coding sequences of UL31 predicts a 35.7 kDa basic protein, and thus the molecular mass of the protein reacted with the UL31 antiserum was co...

Ngày tải lên: 12/08/2014, 04:21

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic histidine. ... avor the displacement of the equilibrium toward the neutral state of His290. To attest this hypothesis, an Arg213Ala mutant has been express...

Ngày tải lên: 07/03/2014, 16:20

... content of the purified PepD wild- type and mutant proteins. Loss of enzymatic activity in the mutants was not due to changes in the second- ary structure of the proteins because the CD spectra of the ... examined the V. alginolyticus PepD protein. In the present study, we present the cloning and expression of the V. alginolyti- cus pepD gene, the purificati...

Ngày tải lên: 23/03/2014, 06:20

... Fig. 8, the truncation of the activation domain eliminated the ability of p67 phox to activate the NBT- reductase activity of the flavoprotein domain. These data indicate that the target of the ... Expression and characterization of the flavoprotein domain of gp91phox 25 2. Biberstine-Kinkade KJ, Yu L, Dinauer MC. Mutagenesis of an arginine- and lysine-r...

Ngày tải lên: 07/08/2014, 14:22

... from humans. The general significance of the study of 2-5A synthetase as one of the key components of the mammalian 2-5A system will be its contribution to our understanding of the evolution of ... demonstrates the results of the purification of the recombinant proteins. The occurrence of dominant bands of the recombinant proteins provides evidence of...

Ngày tải lên: 23/03/2014, 09:20

... maximum of the Soret band with a small absorption coefficient (Table 1). On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the ... exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increas...

Ngày tải lên: 23/03/2014, 20:22

... at the interface between one a subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4]. The c ⁄ e and d subunits play a major role in shaping the ligand-binding ... The purity of the protein was estimated from Coomassie Brilli- ant Blue-stained gels and quantification of the bands using imagej software, and protei...

Ngày tải lên: 30/03/2014, 10:20

... intensity of the bands may be the result of a different copy number of the genes in the hybridizing fragments. If so, the number of the bands do not represent the family size. Thus, the mlp gene ... variants, and this will result in an overestimation of the amount of genomic DNA containing one copy of the mlp gene. Nevertheless, taking into account the po...

Ngày tải lên: 07/03/2014, 15:20

... of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails. A ... outside the Znf domains and in a number of other regions is much lower, even among the mammalian orthologous proteins. HIVEP and the Drosophila Schnurri p...

Ngày tải lên: 30/03/2014, 13:20

Ngày tải lên: 07/08/2014, 20:23