Báo cáo khoa học: " A conserved predicted pseudoknot in the NS2A-encoding sequence of West Nile and Japanese encephalitis flaviviruses suggests NS1'''''''' may derive from ribosomal frameshifting" ppt

... NS1' from a plasmid containing NS1 and the first 60 amino acids of NS 2A (hereafter NS1-NS 2A 1 60 ), they appeared to obtain an NS1' doublet – consistent with a mixture of the zero-frame ... coding sequence, and also at the 5' end of the sequence encoding the NS 2A protein. Such peaks are generally indicative of functionally important non-codi...

Ngày tải lên: 12/08/2014, 04:21

... Nogawa, Daishi Yamada, Ryo Yamazaki and Takahiro Akiyama Laboratory of Cell Biology, Faculty of Environmental Health, Azabu University, Sagamihara, Japan Subsequent to the pioneering work of ... standards; lane A, CBB staining; lane B, Stains-all staining; lane C, negative staining; lane D, Methyl green staining. Arrows on the right side of the lanes indicate the position...

Ngày tải lên: 30/03/2014, 04:20

... changed our experimental setup. Instead of dividing datasets during 10-fold cross-validation into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation ... Another way of classifying ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the...

Ngày tải lên: 20/02/2014, 04:20

... higher than that of the unactivated 2C zymogen, and the difference is again the result of both a decrease in k cat and an increase in K m . The increase in k cat on activation of the 1C and 2C ... ribonucleolytic activity and RI affinity of unactivated and activated 1C zymogen with those of other RNase A variants, we can estimate the therapeutic potent...

Ngày tải lên: 07/03/2014, 11:20

... 5¢-GAAGTTAAAGATACAATGATTCAGCTC 5¢-GAGCTGAATCATTGTAACTTTAACTTC-3¢ 48 N302D 5¢-CATTGTTGAAGACATCAATGTTG-3¢ 5¢-CAACATTGATGTCTTCAACAATG-3¢ 43 N302F 5¢-CTTTACATTGTTGAATTCATCAATGTTGCAGC-3¢ 5¢-GCTGCAACATTGATGAATTCAACAATGTAAAG-3¢ ... 5¢-CAACATTCCTTGTCATCGAACCAAACTC-3¢ 58 R103M 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCATTGTTCTCGAAC-3¢ 55 R103E 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GGTTCGAGAACAGA...

Ngày tải lên: 17/03/2014, 10:20

... the effect of varying the size of the training data. The data point graphed is the average accuracy over the ten samples at a given sample size, with error bars showing the range from the least accurate ... evaluating a system overall, providing feedback to aid in system development, and in evaluating individual sentences. Evaluating an MT system overall Evalua...

Ngày tải lên: 31/03/2014, 04:20

... vasculature, and the urinary tract. In one case an ade- nocarcinoma of the uterine cervix in a transplanted patient was treated initially with Intracavitary BT (low dose rate) followed by a modified ... a kidney graft in a patient with advanced carcinoma of the cervix by reimplantation of the graft from the pelvis to the upper abdomen in preparation for r...

Ngày tải lên: 09/08/2014, 10:21

... such as hemor- rhage, uterine rupture and inversion, and invasion of the urinary bladder, are all related to the site of placental impl antation, the depth of myometrial invasion, and the width of ... describe a case leading to uterine rupture associated with massive intra-abdominal hemorrhage. Case presentation: A 34-year-old Caucasian Albanian woman, gravida 2, par...

Ngày tải lên: 11/08/2014, 02:22

... that the accumulation of fat in cytoplasmic LDs in nonadipose cells, such as glia from the hypothalamus, may be an early sign of dam- age and ⁄ or oxidative stress induced by certain types of NP. ... D’Avila et al. [61]. Thus, it appears that biological and artificial NPs which cause oxidative stress and ⁄ or the release of specific cytokines are potentially hazardou...

Ngày tải lên: 07/03/2014, 00:20

... the PCR gene amplification proce- dures. For bcp1, the forward primer 5¢-TATCTAT CA TATGGTAAAAGTGGGGGA-3¢ and the reverse primer 5¢-AAGAAGGCCAT CTCGAGAGCTGATCT-3¢ contain- ing the NdeI and XhoI restriction ... molecular mass of 16 976.42 Da and a theoretical pI of 8.84. Comparison of the sequences of S. solfataricus Bcps revealed an approximate 35% identity for Bcp1,...

Ngày tải lên: 07/03/2014, 05:20