Báo cáo khoa học: " Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences" docx

... characteristics. AAV is capable of infecting dividing and non-dividing cells in vitro and in vivo and of infecting cells originating from multiple spe- cies and tissue types. No human disease has ... not for citation purposes) Virology Journal Open Access Research Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by inte...

Ngày tải lên: 12/08/2014, 04:21

... changes may trigger the allosteric behavior of Lc. lactis prolidase. Another possibility is that Ser307 works as a key resi- due in the allosteric behaviour of this enzyme. This residue is located ... purification of recombinant Lc. lactis prolidase The overexpression of recombinant proteins can dis- turb host cell metabolism through their activities. To avoid these negative ef...

Ngày tải lên: 30/03/2014, 04:20

... Brassica relative Arabi- dopsis result in high frequencies of dyads and triads through the same mechanism of parallel spindles at meiosis II (Additional file 1) [5,27,32]. A single gene is thought ... recombination: introgression of resistance to pod shatter in Brassica napus . Genetics Research 1990, 56:1-2. 14. Morinaga T: Interspecific hybridisation in Brassica II. The cytology of...

Ngày tải lên: 11/08/2014, 11:21

... element in tRNA Gly 1 -6,7 was significantly distant from the TFIIIC binding region. In tRNA Gly 1 -6,7 binding of TFIIIB, even in the pres- ence of TFIIIC, was dissociated by heparin, unlike TFIIIB ... transcription site is achieved by interactions between the associated factors [16,17]. TFIIIB is analogous to the pol II-specific factor, Keywords Bombyx mori; differential transcr...

Ngày tải lên: 20/02/2014, 02:21

... the expression of the Drosophila a-F1-ATPase gene. Indeed, this is highlighted by the presence of similar combinations of the two tr anscription factors in the promoter proximal region of the orthologous ... Drosophila aF1-ATPase gene. Initially, we mapped the transcription initiation sites by primer extension, S1 mapping analysis and mRNA 5¢- end a mplification. In this way, we...

Ngày tải lên: 07/03/2014, 16:20

... activities, which include osteoid matrix production and mineralization. McCauley & Somerman [49] demonstrated that TGF-b1 inhibits the formation of mineralized nodules in vitro. In addition, ... matrix derivative stimulates proliferation but not differentiation of pre-osteoblastic 2T9 cells, inhibits proliferation and stimulates differentiation of osteoblast- like MG63 cells, and...

Ngày tải lên: 11/08/2014, 20:21

... structure is otherwise permissive for changes [17]. Once in the circulation, the peptide acts to limit gastrointestinal iron absorption and serum iron levels by inhibiting dietary intestinal iron ... of body iron metabolism. Hepcidin is synthesized under the form of a precursor, prohepcidin, which is processed to produce the biologically active mature 25 amino acid peptide. This p...

Ngày tải lên: 18/02/2014, 18:20

... retained within the cells. This inhibition of secretion is caused by the stable association of an endoplasmic reticulum-resident stress protein, BiP, with the H-chain dimer [34–36]. This mechanism ... CH 3 domain interface region distal to the glycosylation site in the CH 2 domain of IgG [29], and the affinity of the IgG-binding proteins for IgG is unchanged by deglycosylation...

Ngày tải lên: 23/03/2014, 04:20

... studies. Kinetic analysis confirmed the enzyme activity of the recombinant protein, and inhibition analysis confirmed classic competitive inhibition by a-glucosidase inhibi- tors. Salacinol with a K i of ... of the lines corresponding to inhib- itor concentration approximately intersected at a point at the y-axis indicating classic competitive inhibition. The experimentally determin...

Ngày tải lên: 07/03/2014, 12:20

... Sanita ` Pubblica Veterinaria, Universita ` degli Studi di Milano, Milano, Italy; 5 Dipartimento di Biotecnologie e Bioscienze, Universita ` degli Studi di Milano-Bicocca, Milano, Italy Gas1p i s a glycosylphosphatidylinositol-anchored ... pathway. Given this prominent role of C74 in directing the folding of Gas1p, it is premature to assess whether it is involved in the formation o...

Ngày tải lên: 16/03/2014, 18:20