Báo cáo y học: "Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone" doc

... 10.1186/1743-422X-7-118 Cite this article as: Chandra et al., Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepati- tis C Virus 2a clone Virology ... replicon cell line expressing the intracellular antibody strongly inhibited the replication of HCV-GFP RNA. Conclusion: Recombinant huma...

Ngày tải lên: 12/08/2014, 04:20

... Virology Research, School of Medicine, Drexel University, USA. His current researches investigate the entry of hepatitis B virus, pathology of HBV infection and hepatocellular carcinoma (HCC). ... Baculovirus IAP repeat (BIR) [20], which probably prevents the proteolytic processing of procaspase-3, procaspase-6, and procaspase-7 by binding and blocking the activity of caspase...

Ngày tải lên: 02/11/2012, 11:17

... Strasbourg, France) and F. Grignani (Univer- sity of Perugia, Italy), respectively. The cDNA was subcl- onedinpCS2-myc-tag 3¢ to a sequence encoding six copies of a 13-residue c- myc epitope. COS-7 cells ... subsequently treated with monoclonal mouse anti- (c- myc-tag) Ig and ¯uorescein isothiocyanate-conjugat- ed (FITC-conjugated) goat anti-(mouse IgG) Ig. The cells were examined by ¯uo...

Ngày tải lên: 08/03/2014, 16:20

... double bonds of a wide variety of substrates including steroids. Such types of reactions have been proposed to occur in the biosynthetic pathway of the cardiac glycosides produced by Digitalis ... blot analysis revealed that DpARs are encoded by a family of at most five genes. A characteristic function of the ARs is the catalysis of the first reaction in the polyol pathway, the re...

Ngày tải lên: 18/03/2014, 01:20

... 5′-GGC CAC CAC GAC CAC CAC CAC-3′, at 62 C and for 32 cycles, were used for CXCR3-specific amplification. CXCL9 mRNA was detected after 29 cycles with the primers 5′-GGA GTG CAA GGA ACC CCA GTA-3′ ... lanes correspond to individual patients within Table 1. (b) Quantitation of the expression of Cys–X–Cys receptor (CXCR)1, CXCR2, CXCR3, T-cell receptor (TCR)-ζ, Cys–X–Cys ligand (CXCL)9,...

Ngày tải lên: 09/08/2014, 01:23

... 5' GCA GTT TGC TCA GCC TAT CC 3' and 2) 5' GAG TGT CGG AGT CCA GCT TC 3' [26]; GAPDH (glyceraldehyde-3-phosphate dehydrogenase), 1) 5' AGC AAT GCC TCC TGC ACC ACC AAC 3' ... (Clontech), developed specifically for analysis of the Atlas cDNA expression arrays. All spots were individu- ally checked by hand to ensure accuracy of the detection method. A dot was...

Ngày tải lên: 09/08/2014, 06:22

... G-protein-coupled receptors classified as C, CC, CXC, or CXXXC chemokine receptors according to the type of chemokine bound. Chemokine receptors are constitutively expressed on some cells, whereas they ... specific receptor (CXCR3) [2]. Although the interactions of chemokine receptors are often charac- terized by considerable promiscuity, CXCR3 is selective in the recruitment of Th1 ce...

Ngày tải lên: 09/08/2014, 06:22

... GGC CGA TGG GCT GGA CAG 793 62 MMP-3 J03209 TGG AGC TGC AAG GGG TGA GGA CAC CAG GCG GAA CCG AGT CAG GTC TGT 691 62 BMP-4 M22490 ACC CGG GAG AAG CAG CCA AAC TAT AGC GGC ACC CAC ATC CCT CTA CTA 553 ... 62 TNF-α M10988 CTC TGG CCC AGG CAG TCA GA GGC GTT TGG GAA GGT TGG AT 519 62 IL-1β M15330 CAC CTG TAC GAT CAC TGA ACT GCAC GGC TGG GGA TTG GCC TGC AA 674 60 MMP-1 X05231 CTG CTG CTG TTC TGG GGT G...

Ngày tải lên: 09/08/2014, 07:20

... 7 Atorvastatin-induced CD59 and decay-accelerating factor in hypoxia enhance endothelial cell cytoprotectionAtorvastatin-induced CD59 and decay-accelerating factor in hypoxia enhance endothelial cell cytoprotection. ... were as follows: β-actin forward, GAGCTACGAGCTGCCTGACG; β-actin reverse, GTAGTTTCGTGGATGCCACAGGACT; CD59 forward, ATGCGTGTCTCATTAC; and CD59 reverse TTCTCTGA- TAAGGATGTC. The...

Ngày tải lên: 09/08/2014, 08:22

... NM_000618 Antisense CCTGTCTCCACACACGAACTG IGF-II Sense GAGGAGTGCTGTTTCCGCAG 263 62 27 NM_000612 Antisense ACGTTTGGCCTCCCTGAACG TGF- β 1 [53] Sense CTAGACCCTTTCTCCTCCAGGAGACG 224 62 26 NM_000660 Antisense GCTGGGGGTCTCCCGGCAAAAGGT COL1A1 Sense ... GCTGGGGGTCTCCCGGCAAAAGGT COL1A1 Sense CGGCAAGGTGTTGTGCGATG 339 62 33 NM_000088 Antisense CACGGAAATTCCTCCGGTTG COL1A2 Sense CGCTGGTGAAGTTGGCAAACCA 778...

Ngày tải lên: 09/08/2014, 08:23