báo cáo khoa học: " Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea" doc

... values of abundance index of AcH4 (± SE). Lane 1: tubulin and AcH4 of bud in phase A; Lane 2: tubulin and AcH4 of bud in phase B; Lane 3: tubulin and AcH4 of bud in phase C; Lane 4: tubulin and AcH4 ... 7 Intensity of AcH4 fluorescence markers of buds in phase A and phase B. (a) AcH4 labelling of bud in phase A (10×). (b) Intensity of AcH4 fluores...

Ngày tải lên: 12/08/2014, 03:21

... ole in the regulation of cell proliferation, including SMC, by inhibiting signalling mol- ecules [ 45–47]. Caveolin-3 is the main isoform in muscle cells, including SMC [30]. Caveolin-1 and caveolin-3 ... production and concentra- tions of cyclin D1, Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation. This article describes...

Ngày tải lên: 19/02/2014, 16:20

... min after the fans were turned off in the evenings in order to maintain higher concentrations in the chambers. [CO 2] was mea- sured continuously in both the ambient and ... The seedling containers were assembled in racks and wrapped in aluminum foil to avoid root system overheat- ing, and set in trays constantly containing a layer of...

Ngày tải lên: 08/08/2014, 14:21

... behaviour Walking/running Moving walking, trotting or galloping By udder Activity by the udder: suckling, massaging udder or looking for a teat Nosing/chewing/ licking Nosing/chewing or licking material ... the littermates/mother Playing Head shaking, springing (sudden jumping or leaping) or running. Can involve partners (gentle nudging or pushing, mounting, chasing, etc.) Sleeping Eyes close...

Ngày tải lên: 12/08/2014, 18:22

... release of cytochrome c from mitochon- dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3, the phosphoserine ⁄ threonine binding proteins in ... by containing a Ser139 phosphorylation site in the C-terminal tail. Phosphorylation of H2AX on Ser139 is a key event in the repair of DNA damage and the induction of DNA...

Ngày tải lên: 14/02/2014, 22:20

... proteins. Immunostaining of meiotic C. cinereus nuclei Immunostaining of meiotic C. cinereus nuclei was perfomed as described in previous reports [29–31] with minor modi- fications. C. cinereus ... and p48 The truncated cDNA corresponding to amino acid residues from 299 to 718 of p140 was cloned into the expression vector pET21a (Novagen) in the NdeIandHindIII sites. The coding reg...

Ngày tải lên: 20/02/2014, 11:20

... examined the DNA- binding activity of Mhr1 in the absence of Mg 2+ , because the DNA- binding activities of other HP proteins are often increased by the addition of Mg 2+ in vitro [31,32]. In the ... may explain our difficulty in obtaining a DNA- binding- deficient mutant via a single-site mutation. Next, we focused on the Mg 2+ -dependent DNA- binding activity of Mhr1,...

Ngày tải lên: 06/03/2014, 09:22

... AAA + domains of both proteins are shown in cyan with ADP indicated in red. The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix (WH)-containing domain of ... crystal structures of DnaA [59] and Orc1 ⁄ Cdc6 proteins [60,61]. As can be seen in Fig. 4, both proteins possess N-terminal AAA + domains and C-terminal DNA binding...

Ngày tải lên: 07/03/2014, 21:20

... Then, photoresponsive DNA rich in the cis form was mixed with the template strand and incubated at 37 °C for 30 min in the dark. To achieve cis–trans and trans–cis isomers, a solution containing ssDNA involving ... introduced into a T7 promoter instead of Azo (Fig. 1). Clear-cut on–off photoregulation of DNA transcription was obtained because of the efficient suppression of...

Ngày tải lên: 15/03/2014, 10:20

... interstrand cross-linking efficiency of the new analogue of transplatin was almost independent of r b and was 26%. Interestingly, interstrand cross- linking efficiency of parent transplatin was ... being trans- formed into the interstrand CL. After 24 h of incubation of the duplex TGTGT containing the 1,3- intrastrand CL of trans-Pt-HMP, 12% of the 1,3- intrastrand CLs were...

Ngày tải lên: 16/03/2014, 14:20