... 42095; staining I: safranine, 60 min; washing: H 2 O, 2 min; staining II: pyoctanin blue, 3 min; washing: H 2 O, 5 min; washing: isopropanol, 1 min: staining III: acid green, 1 min; 4× washing: isopropanol, ... [12]. Regarding the indistinct descriptions and the lack of cur- rent in- depth studies and molecular data in C. vulgaris, several uncertainties still exist on the topic...
Ngày tải lên: 12/08/2014, 03:21
... con- taining atRE (A), liposomes containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 mM FeSO 4 (C). The generated retinoids were ... in (B4) showing RPE65c staining (B5), GS staining (B6), merged RPE65c and GS staining (B7), and DAPI staining (B8), respectively. White arrows indicate overlapped signa...
Ngày tải lên: 14/02/2014, 14:20
Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
... size of proteins enriched in the lipid-rich pellet including the major 29 kDa protein Por1 and Tom40, identified by mass spectrometry and immunoblotting. (C). After in situ digestion with trypsin, ... Coomassie-staining the gels, bands representing proteins of interest were excised and mounted into an electroelution chamber, with a BT2 mem- brane (Schleicher & Schuell, Bottmi...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... the binding of target proteins occurs in the groove between monomers, and involves a b-sheet region located at the beginning of the a-crystallin domain cor- responding to residues 70–88 in aA-crystallin ... importance of each of the negatively charged residues in the C-terminal extension in maintaining the solubility of the Hsp25 oligomer. Similarly, the introduction of...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
... polysaccharide binding site. However, it is intriguing to observe long blank spaces of 11 A ˚ in length and 14 A ˚ in length between subsites 1 and 2 and 2 and 3, respectively. An examination of these regions ... to obtain insight into the regions that are critical for ligand binding. The substrate of this enzyme is a polysaccharide consisting of repeating units of 2-ace...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx
... calcium binding are shown in orange and those involved in nucleotide binding are shown in red. Residues involved in the binding to the PLP moiety are shown in blue, whereas those involved in protein–protein ... a different PDZ domain- containing protein was found to interact with serine racemase, also requiring the C-terminal binding motif [30]. Protein interacting with C kina...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: "Wood density traits in Norway spruce understorey: effects of growth rate and birch shelterwood densit" ppsx
... tempera- ture and radiation indices and their adjustment to hor- izontal and inclined forest land, Stud. For. Suec. 194, 1994. [35] Nakagawa S., Influence of pruning and light intensity ... indicating site index G 18, i.e. an 18-m dominant height of Norway spruce at 100 years of age [14]. Following clear-felling and prescribed burn- ing in...
Ngày tải lên: 08/08/2014, 14:21
báo cáo khoa học: "Bilateral adrenocortical carcinoma in a patient with multiple endocrine neoplasia type 1 (MEN1) and a novel mutation in the MEN1 gene" potx
... and midre- gion (amino ac ids 323-428) of Menin and requires the N-terminus of JunD (amino acids 8-70). Menin’stumor suppressor involves direct binding to JunD and inhibi- tion of JunD activated ... for proper nuclear targeting. Menin contains two NLSs. Both NLS-1 (amino acid 479-497) and NLS-2 (amino acids 588-608) are present in the C-fourth of menin [35]. Menin binds to...
Ngày tải lên: 09/08/2014, 01:24
báo cáo khoa học: "Relationship between densityand in two strains of frequency-dependent effects Drosophila melanogaster GONZÁLEZ-CANDELAS" docx
... and methods A. Strains and culture conditions Two strains of Drosophila melanogaster were used : a wild strain, Oregon-R (isogenic) and an eye colour mutant strain, cardinal ... to the degree of the fitted equation, and the degrees of freedom in the denominator are the product of the number of classes minus 1 and the number of re...
Ngày tải lên: 09/08/2014, 22:22
báo cáo khoa học: "Allozyme frequency changes in two inverse sequences of environments in Drosophila melanogaster" ppt
... to thank Professor C. PETIT for her trust and critical reading of the manuscript, and is very grateful to Professor C. KR IMBA S for his continuing interest and helpful ... histories and in the KC (PGM F increased, Pgm v decreased and Pgm s decreased or remained stable). Allozyme frequency changes in two inverse sequences of environme...
Ngày tải lên: 09/08/2014, 22:23