báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx
... cgtc gac CAT CCC TGT ACC ACT CAC TAT CTT ATT C -3’ and 5’ - GAA TCC GAA AA A ATT GAA CCT GAG AC-3’ ,and5 ’-acg cgt cga cTA ACA CAC AAA ATG TAC CGA AAG AAG-3’ and 5’- CTG TGT AGA TTT CTG AGA GGA GGG ... CTA GAA TGA GCT CAT CTG ATT CCG TTA ATA ACG G-3’ and 5’-AAC TAA TTA TAT CCG ATT ATC AGA ATA AG-3’.The fragment was treated with Xba I and ligated with GUS- less pBI121, which was prep...
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
... ACCggTTCCggTggTggTgTgATgAACC DX46-GSP2 ACTCATCAgTCTTAgTAggCTCgggTgTT THA82-GSP1 TgATTTTAgCTgCATgTgCACCTgAgAA THA-1-GSP2 CgTCATggAAATgTCTTAATTggCTTgCTg LOX101-GSP1 gAAgAAAACAAATTgggAggAggAgAA LOX63-GSP2 gCgTgTTCCAAAgAACACAATTCAgTgCCTT BEC-32BamHI ... AAggAgCTCTTgACgTTggA LOX1267R TgCTAACAggTgggAAAACC ACT-F CCTTCCAgCAgATgTggATT ACT-R AgATTAggCAAggCgAggAT BEC87-GSP1 TgCATTTCCAgCTTgCCTCCCACATTg G...
Ngày tải lên: 12/08/2014, 03:21
báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt
... TTG TTA GTG CTT G D3-F CCC AAGCTT GAA CAA TTT TCC TCA AAG TTG TAG D-R CG GGATCC AGA AAA TGT AAA GAT TGA TAA TGG CaMV35S-F GAA TGC TAA CCC ACA GAT GGTTAG GUS-R GTT CAA CGC TGA CAT CAC CAT TG CYC-B-F ... NNN NA TGC Walker2 CTA ATA CGA CTC ACT ATA GGG NNN NT AGC Walker3 CTA ATA CGA CTC ACT ATA GGG NNN NG ATC Walker4 CTA ATA CGA CTC ACT ATA GGG NNN NC TAG Adaptor walker CTA ATA CGA CTC A...
Ngày tải lên: 12/08/2014, 03:21
báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx
... 5'-TTCCGTTTCGTTTCTTCAA-3' UTR3' 5'-TGGCCATAACCATTTTAGATAT-3' HCTFor 5'-GGGTTTCATATGAAGATCGAGGTGAGAGAA-3' HCTRev 5'-CGGGATCCTTAGATATCATATAGGAACTTGC-3' ART3For 5'-TCCCCAATTTTCACACAC-3' ART3Rev ... 5'-AACGTTHCCRAARTANCC-3' ART2For 5'-ATGGCAACACTGTCAATTA-3' ART2For-nested 5'-CCCGACGATCAGGATA-3' ART2Rev 5'-AC...
Ngày tải lên: 12/08/2014, 05:20
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... molecular mass was also estimated by SDS–PAGE. Characterization and comparative analyses of HYD Js and HYD Bp The optimal temperature for activity of HYD Js with d-p- HPH as substrate was determined ... molecular basis of enzyme thermosta- bility. J Bacteriol 185, 4038–4049. 20 Nanba H, Yajima K, Takano M, Yamada Y, Ikenaka Y & Takahashi S (1997) Process for producing d-N-ca...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
... the cloning and functional characterization of an AK gene from a genomic library of L. donovani as well as its expression and molecular characterization. Materials and methods Materials Plasmids pGEM-3Zf(+) ... Villa 1 , Yolanda Pe ´ rez-Pertejo 1 , Carlos Garcı ´ a- Estrada 1 , Rosa M. Reguera 1 , Jose ´ Marı ´ a Requena 2 , Babu L. Tekwani 3 , Rafael Balan ˜ a- Fouce...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... analysis Data was presented as mean ± SEM, and analyzed using one-way analysis of variance and appropriate post hoc test or by Student’s t-test using PRISM 4.00 software (GraphPad Software, San Diego, ... P763 (5¢-TTCTCGAGACGCGTTATCGATAGAGAAATGT TCTGGC-3¢) and digested the PCR product with EcoRI and XhoI. The insert was synthesized with primers P764 (5¢-AACTCGAGGCTAGTCTGCAGGAGCTCA...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... VBARP-L and VBARP-S variants have high homology to PP2500 and ANKHD1 vari- ant 2. In this study we focus on the biochemical and functional characterization of the novel VBARP-L and VBARP-S transcripts. ... 5¢-CTAGACTCGAGCCTAAT TTATATTTGCTCCTTGTGC-3¢. b-Actin primers were designed as follows: forward 5¢-CTACAATGAGCTGCG TGT-3¢ and reverse 5¢-AAGGAAGGCTGGAAGAGT-3 ¢. Cell survival...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... of the larger RNA fragments by lamjapin was not artifact caused by the nuclease contaminant. It was also shown that the ratio of fragments Fig. 3. Activity of lamjapin. (A) Effect of lamjapin on ... A4 324 of rat ribosomal RNA was flipped [27]. Lamjapin could deadenylate A2 0 of SRD RNAandreleasedtheRNAfragmentwiththeexactsizeof the R-fragment from the ribosomal RNA. It is l...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... 855–862. 2 Kawasaki H, Nakayama S & Kretsinger RH (1998) Classification and evolution of EF-hand proteins. Bio- metals 11, 277–295. 3 Ohsawa K, Imai Y, Kanazawa H, Sasaki Y & Kohsaka S (2000) ... core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands 1 and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) flanking a loop region able to bind...
Ngày tải lên: 16/03/2014, 06:20