báo cáo khoa học: " New sources of soybean seed meal and oil composition traits identified through TILLING" docx

... article New sources of soybean seed meal and oil composition traits identified through TILLINGEmily C Dierking1 and Kristin D Bilyeu*1,2Address: 1University of Missouri-Columbia, Division of ... novel sources of important soybean seed composition traits confirms the utility of this reverse genetics technology as a route for both genefunction analysis and direct applicability for soybean improvement. ... progenitor of line 17D.Several other lines were grown for comparison because of Inheritance of S117N alleles of FAD2-1A results in increased mean oleic acid and decreased linoleic acid soybean seed oilFigure...

Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... constants of 0.77 ± 0.04 min)1 and 0.72 ± 0.02min)1, and of 0.32 ± 0.03 min)1 and 0.19 ± 0.02 min)1could be calculated, respectively, for hydroxylamines 1 and 2 in the case of MP8 and 3A3–MP8. ... consistent with the insertion of the MP8–Fe(II)–RNO complex into a hydrophobicpocket with no change of the Fe(II) spin state and noreplacement of any of the two axial ligands of the iron,His18 or ... side-chain of histidine 18 acts as aproximal ligand of the iron atom. A set of six monoclonalantibodies was thus obtained: the best peroxidase activity –that found with the complex of MP8 and one of...

Báo cáo khoa học: New application of ﬁreﬂy luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... resolution of (R,S)-ibuprofen and (R,S)-ketopro-fen by microbial lipases from native and commercial sources. Brazil J Microbiol 37, 329–337.26 Yonekubo J, Inoue T & Sasaki H (2006) Feature of newest ... theremaining acid and ﬂurbiprofen as an internal stand-ard using reversed phase HPLC analysis. According tothe Lineweaver–Burk plot, the Km and Vmaxvalues forracemic ketoprofen were 0.22 mm and 110.3 ... magnitude of afﬁnity. The Michaelis–Men-ten parameters of recombinant rat LACS1 for the thio-ester formation were reported by Sevoz et al. [16] and the values of Km and Vmaxfor (R)-ibuprofen and...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Blue-light active ﬂavoproteins studied by electron paramagnetic resonance pptx

... Hence,understanding the suitability and potential of crypto-chromes for magnetoreception requires identiﬁcation of radical pair states and their origin, and the detailedcharacterization of magnetic ... basis of the correlated coupled radical pairmodel and assuming the ﬁxed orientations of theTrp324•radical and the ﬂavin radical given by the 3Dstructure of the protein [86]. The strength of ... studies of paramagnetic ﬂavin species, the applica-tion of EPR has traditionally been valuable to distin-guish the protonation state of ﬂavin semiquinones bymeans of the signal width of its...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... The volumes of the cavities are601, 313, 637 and 1736 A˚3for PAO, MAON, MAO B and LSD1, respectively. (B) Over-view of the active site surrounding the ﬂavincofactor in each of the four ... part of LSD1 is predictedto be a disordered region and is discussed in the nextsection.The active site of all these amine oxidases is nor-mally located in the proximity of the ﬂavin ring of ... enzyme func-tion and type of substrate. PAO is outstanding in thisregard because it is endowed with a long tunnel suitedto the aliphatic chain of polyamines (Fig. 3) [30] and its yeast homolog...

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx

... particle of the proteasome and recruits certain transcription fac-tors such as p53 and p73a to the complex. The latter process appears to begoverned by the redox state of the ﬂavin cofactor of the ... Apparently,oxidation of the ﬂavin cofactor of Lot6p results in thedissociation and concomitant relocalization of thereleased transcription factor to the nucleus whereexpression of stress related ... role of the second human QR in regula-tion of p53 and found that loss of NQO2 signiﬁcantlydecreases the level of p53 [39]. Co-immunoprecipita-tion studies revealed a physical interaction of...

Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... family is of great interest in terms of structure–function relationships and the evolution of serine proteases. Reports of new members provide amore complete picture of the family and potentiallycan ... widevariety of primary speciﬁcities and no member of this family has beenreported for spiny lobsters. The aim of this work was to study the diversity of trypsins in the digestive gland of Panulirus ... The aim of the present work wasto study the diversity of trypsins in the digestivegland of P. argus, with focus on: (a) the position of lobster trypsins within the brachyurins family and (b)features...

Báo cáo khoa học: " Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes" potx

... to Soybean mosaic virus in Zao18 soybean from China. J Hered 2002, 93:447-452.4. Babu M, Gagarinova AG, Brandle JE, Wang A: Association of thetranscriptional response of soybean plants with soybean mosaic ... ZD: Identification of two new starins of soybean mosaic virus. Acta Phytophyl Sin 1986,13:221-226.11. Pu ZQ, Cao Q, Fang DC, Xi BD, Fang CT: Identification of strains of soybean mosaic virus. ... of Canada. AGG was a recipient of Ontario Graduate Scholarship and Western Graduate Research Scholarship.References1. Arif M, Hassan S: Evaluation of resistance in soybean germ-plasm to Soybean...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... 6, Glu-Pg- and mAb 34D3-trea-ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). High molecular weight bands in lanes 5 and 6 correspond tomAb A10.2- and mAb 34D3-plasminogen ... the presence of varying concentrations of mAb A10.2 or mAb 34D3 (0–1 lM for cells and 0–0.25 lM for ﬁbrin) and ﬁxedconcentrations of Glu-Pg (200 nM for cells and 50 nM for ﬁbrin) and CBS0065 ... were starved of serum and then incubatedwith varying concentrations of Glu-Pg (0–2 lm) and a ﬁxedconcentration (0.75 mm ﬁnal concentration) of CBS0065 ina total volume of 100 lL of Ham-F12 medium...

Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx

... the coiled-coil domains I and II of laminins[1,2], whereas o ther domains, such a s those composed of Fig. 6. Immunohistochemical localization of laminin b2 chain in basement membranes of adult ... of LN and LE (circles) modules, domain IV, and a coiled-coil (cc) domainII,I.Fig. 2. SDS/PAGE of puri®ed recombinant fragments c ontainingdomain IV of b1orb2 chain under nonreducing (A) and ... anti-(rat Ig) IgG and were all negative.RESULTSExpression and puri®cation of recombinant domain IVfrom mouse laminin b1 and b2 chainDomain IV of t he b1 (position 541±771) and b2 (position556±782)...

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