... straight lines, confirming the applicability of the Nicholson and Shain theory to the present system, and the variation of the pseudo-first order rate constant with CuNirconcentrations (between ... center, the proton of this hydrogenbond is transferred from the Asp98 residue to the oxygenatom of the substrate, yielding an O¼N–O–H intermediate. The N–O bond in this oxygen atom is then broken, ... yielding the product NO, whose release re-establishes the originalcoordination of the active center. HisB255 could be involved in the formation of additional hydrogen bonding, stabilizingthe...