báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... DnaJ-For5'GGAATACAGGAGGGG GA CAT, Rev5'CCTTTTGGGAGAACCAAACA; BADH-For5' TGGAAAATTGCTCCAGCTCT, Rev5'CTGGACCTAATCCC GTCAAA; Actin-For5'AAACCACAAGCCCCTAAACC, Rev5 'TTGCATCACTCAGCACCTTC. ... genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization Binod B Sahu* and Birendra P Shaw Address: Environmen...
Ngày tải lên: 12/08/2014, 03:20
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GT- cDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp). Construction and screening of a grass ... 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly- peptide of 533 amino acids, with a predicted molecular mass of 57 kDa and a pI of...
Ngày tải lên: 17/03/2014, 03:20
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... reverse2 GAGGCTAGATACTGCTCGATGT IL-10 forward3 TGATGATTTGGAACCATTATTGAA IL-10 reverse3 CACCTTTTTCCTTCATCTTTTCAT b-Actin forward1 ACTACCTCATGAAGATCCTG b-Actin reverse1 TTGCTGATCCACATCTGCTG T7- forward ... characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. Ram Savan 1 , Daisuke Igawa 2 and Masahiro Sakai 2 1 United Graduate School of...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc
... GCTCCGAATTCGAACATGAC IL-18B expression ER1 AGGCAAAGGTTGCTCCAGTG IL-18 expression Actin-F ATGGAAGATGAAATCGCC Gene expression Actin-R TGCCAGATCTTCTCCATG Gene expression RR1 TGGTACCACTCAACATGTCAGTAAGCG ... 5¢-RACE F1 GCAATGCGACCGAGTGTCGGAG 3¢-RACE Gene organization F2 CGACATTTCCGAGTGACGTTC 3¢-RACE R2 CCTTCAACACCCTGACTTCAC 5¢-RACE R3 ATGTCCTCCTTGTCTACTACC 5¢-RACE EF1 AGCAGCTCCGAATGTAAGGTG...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... is a single copy of RNase j in the C. capitata genome. The genomic DNA frag- ATG TAA Poly A Poly A AAUAAA AAUAUA Gene mRNA 01 PA S1 PA S2 mRNA 02 100 bp Fig. 6. Schematic organization ... capitata as described previously [40] as template and the DS74 (5¢-TTG TGGAAAATCATACGAGA-3¢) and R1286 (5¢-CAAAC ACACATCGAGGAGC-3¢) oligonucleotides corresponding to the 5¢ and 3¢ t...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: "Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis" ppsx
... develop a range of pathologies, including hepatocellular carcinoma, cochlear hair cell loss, vascular dysfunction, anemia, acceleration of age-related loss of muscle mass, an earlier incidence of ... performed using an anti-SOD1 antibody. β-actin was used as an internal standard. Materials and Methods Experimental animals Male and female ICR mice (8 to 10 weeks old) were...
Ngày tải lên: 07/08/2014, 20:23
Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... g50c (5¢-TTGATAGTTATCTATTACAG TCTTCTTAGATTGAAACAA-3¢), g177 (5¢-CATACAA ACATAATAAGATGTAAATGG-3¢) and control g177c (5¢-TCATCTAAGTACAATAGATAGAAGAAA-3¢), g230 (5¢-TGTTACTCTCCAAGCAAC CA-3¢) and control g230c ... TGGAAAGCTTGCCACATCAGTCTACAAAG 85R TGCTCCATGGTGGCATCCCAATATGTAAACCA 83 83F GAACCAAGCTTGAAGCACATTCTTGCAGTAAGCA 83R CAAAACATGTTGGCTACGGGACATACAAATGTTCA 80 80F GTTGAAGCTTTTTGAAACTTAGCACT...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
... FF5, matching with the N-terminal sequence of the mature protein: 5¢TAT GCGTGGTCCAATGCTATGC, and FF 3A, matching with the C-terminal sequence of the coding region: 5¢TTAC AAGGACAAATTAATTGTGCCAG. ... Pru av 3 and the 15-kDa band shows reactivity using a polyclonal rabbit serum against profilin from pear, Pyr c 4 (data not shown). These results indicate that also LTP and profilin ma...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... GCA GCC GCC CAG GAG GTC CAG ACC GCC GCC 156 53QEAVEGAEQVASESASLTTP CAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA ... 336 113KAIQDILNEHNMFRAKERVP AAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG 396 133PLTWNTTLAKFSQDYAESKL CCG CTC ACG TGG AAC ACG ACG CTT GCC AAG TTC TCG CAG GAC TAC G...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx
... 2B). Purification of TyrAAT1 and TyrAAT2 The two recombinant isoforms of A. thaliana arogenate dehydrogenase TyrAAT1 and TyrAAT2 lacking their putative plastidic transit peptide were overproduced in the E. ... acid sequence of the two protein domains of TyrAAT1 (TyrAAT1.1, and TyrAAT1.2), and TyrAAT2 using the CLUSTALW program (B). (A) Putative transit peptide cleavage si...
Ngày tải lên: 17/03/2014, 11:20