báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc

... DnaJ-For5'GGAATACAGGAGGGG GACAT, Rev5'CCTTTTGGGAGAACCAAACA; BADH-For5'TGGAAAATTGCTCCAGCTCT, Rev5'CTGGACCTAATCCCGTCAAA; Actin-For5'AAACCACAAGCCCCTAAACC, Rev5'TTGCATCACTCAGCACCTTC. ... genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridizationBinod B Sahu* and Birendra P ShawAddress: Environmental Biotechnology Laboratory, Institute of ... domain in AMPK has greater affinity forAMP than for ATP, and as the cellular energy contentdrops (low ATP, high AMP), binding of AMP to CBSdomain of AMPK facilitates its phosphorylation makingthe...

Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

... GT1-R,5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GT-cDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTACCTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTTCCAAGTGC-3¢ for GT-cDNA2 (3043 bp).Construction and screening of a grass ... 11 introns, and anopen reading frame (ORF) of 1599 bp encoding a poly-peptide of 533 amino acids, with a predicted molecular mass of  57 kDa and a pI of 8.34.BLASTX analysis showed thatthe ... digestedwith Sau3AI and fragments larger than 9.5 kb were ligatedinto BamHI-digested EMBL3 arms (Stratagene) and pack-aged into Escherichia coli XL1-Blue MR cells using Gigapack Gold Packaging Extract...

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... reverse2 GAGGCTAGATACTGCTCGATGTIL-10 forward3 TGATGATTTGGAACCATTATTGAAIL-10 reverse3 CACCTTTTTCCTTCATCTTTTCATb-Actin forward1 ACTACCTCATGAAGATCCTGb-Actin reverse1 TTGCTGATCCACATCTGCTGT7- forward ... characterization and expression analysis of interleukin-10from the common carp,Cyprinus carpioL.Ram Savan1, Daisuke Igawa2 and Masahiro Sakai21United Graduate School of Agricultural Sciences, Kagoshima ... forward TAATACGACTCACTATAGGGSP6-reverse ATTTAGGTGACACTATAGAAFig. 1. Genomic sequence structure of carp IL-10. Coding sequences are shown in uppercase, whereas the untranslated region (UTR) and introns...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... GCTCCGAATTCGAACATGAC IL-18B expression ER1 AGGCAAAGGTTGCTCCAGTG IL-18 expression Actin-F ATGGAAGATGAAATCGCC Gene expression Actin-R TGCCAGATCTTCTCCATG Gene expression RR1 TGGTACCACTCAACATGTCAGTAAGCG ... 5¢-RACEF1 GCAATGCGACCGAGTGTCGGAG 3¢-RACEGene organizationF2 CGACATTTCCGAGTGACGTTC 3¢-RACER2 CCTTCAACACCCTGACTTCAC 5¢-RACER3 ATGTCCTCCTTGTCTACTACC 5¢-RACEEF1 AGCAGCTCCGAATGTAAGGTG IL-1 8A expression EF2 ... Sugawara, S., Uehara, A. , Nochi, T., Yamaguchi, T., Ueda, H.,Sugiyama, A. , Hanzawa, K., Kumagai, K., Okamura, H. &Takada, H. (2001) Neutrophil proteinase 3-mediated induction of bioactive...

Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... is a single copy of RNase j in the C. capitata genome. The genomic DNA frag-ATG TAA Poly A Poly A AAUAAA AAUAUA Gene mRNA 01 PA S1 PA S2mRNA 02 100 bp Fig. 6. Schematic organization ... capitata as describedpreviously [40] as template and the DS74 (5¢-TTGTGGAAAATCATACGAGA-3¢) and R1286 (5¢-CAAACACACATCGAGGAGC-3¢) oligonucleotides correspondingto the 5¢ and 3¢ terminal regions ... of analytical grade and obtained fromMerck (Darmstadt, Germany).Isolation of the Cc RNase cDNA 02 cloneTotal RNA was prepared from 6-day-old larvae of theinsect C. capitata (Bouhin et al....

Báo cáo khoa học: "Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis" ppsx

... develop a range of pathologies, including hepatocellular carcinoma, cochlear hair cell loss, vascular dysfunction, anemia, acceleration of age-related loss of muscle mass, an earlier incidence of ... performed using an anti-SOD1 antibody. β-actin was used as an internal standard.Materials and MethodsExperimental animals Male and female ICR mice (8 to 10 weeks old) were purchased from a commercial ... chronic inflammation, tissue damage, and bone damage associated with collagen-induced arthritis [5]. Thus, SOD1 may act as an important antioxidant in osteogenesis and hematopoiesis during embryonic...

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... g50c (5¢-TTGATAGTTATCTATTACAGTCTTCTTAGATTGAAACAA-3¢), g177 (5¢-CATACAAACATAATAAGATGTAAATGG-3¢) and control g177c(5¢-TCATCTAAGTACAATAGATAGAAGAAA-3¢),g230 (5¢-TGTTACTCTCCAAGCAAC CA-3¢) and controlg230c ... TGGAAAGCTTGCCACATCAGTCTACAAAG85R TGCTCCATGGTGGCATCCCAATATGTAAACCA83 83F GAACCAAGCTTGAAGCACATTCTTGCAGTAAGCA83R CAAAACATGTTGGCTACGGGACATACAAATGTTCA80 80F GTTGAAGCTTTTTGAAACTTAGCACTTCTGC80R ATTCCATGGTGGCTGAAATCATTTCATTTTGATTGCC74 ... CATGATTTCACGTTTAGCTCAATTTAAGCCAAGTTCCCAAATTTTAAGAAAAGTAG-3¢ and 5¢-TCGACTACTTTTCTTAAAATTTGGGAACTTGGCTTAAATAAACGTGAAATCATGGTGGC-3¢) were annealed by heat-ing a 4-lMmixture of primers to 95 °C for 3 min followedby...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... FF5, matchingwith the N-terminal sequence of the mature protein: 5¢TATGCGTGGTCCAATGCTATGC, and FF 3A, matchingwith the C-terminal sequence of the coding region: 5¢TTACAAGGACAAATTAATTGTGCCAG. ... Pru av 3 and the15-kDa band shows reactivity using a polyclonal rabbitserum against proﬁlin from pear, Pyr c 4 (data notshown). These results indicate that also LTP and proﬁlinmay be relevant ... fractionscontain a contaminating band at 90 kDa that was notdetected in the silver stained SDS/PAGE gel but seems to beIgE reactive with almost all sera tested. N-Terminalsequencing analysis failed because...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... GCA GCC GCC CAG GAG GTC CAG ACC GCC GCC 15653QEAVEGAEQVASESASLTTPCAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA ... 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG 396133PLTWNTTLAKFSQDYAESKLCCG CTC ACG TGG AAC ACG ACG CTT GCC AAG TTC TCG CAG GAC TAC GCG GAG TCG AAG CTG ... 456153KKDCKMVHSDSPYGENLMFGAAG AAG GAC TGC AAG ATG GTG CAC TCG GAC TCG CCC TAC GGG GAG AAC CTG ATG TTC GGC 516173SGAISWKTTVDTWSDEKKSYTCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC...

Báo cáo khoa học: Puriﬁcation and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx

... 2B).Puriﬁcation of TyrAAT1 and TyrAAT2The two recombinant isoforms of A. thaliana arogenatedehydrogenase TyrAAT1 and TyrAAT2 lacking theirputative plastidic transit peptide were overproduced in theE. ... acidsequence of the two protein domains of TyrAAT1 (TyrAAT1.1, and TyrAAT1.2), and TyrAAT2 using theCLUSTALWprogram (B). (A) Putative transit peptide cleavage site determined using CHLOROPpro-gram are ... Schematic diagram of the amino acid sequence of the two A. thaliana arogenate dehydrogenases TyrAAT1 and TyrAAT2 deducedfrom their respective coding sequences (A) and alignment of amino acidsequence...

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