... °C, in 1 L of Luria–Bertani broth containingampicillin (100 mg L )1 ). When the attenuance (D) reached0.3 at 600 nm, the cells were induced by the addition of 0.1 mMisopropyl thio-b-D-galactoside ... NHase.Thewild-typeenzyme,aT109S mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively. The Cc2atomofaThr109 and the Cc1atomofaVal136 of the Co-typeNHase are connected by a yellow line. ... the BL6A station of the Photon Factory(Tsukuba, Japan) and the BL38B2 and BL40B2 stations of SPring-8 (Harima, Japan), at 100 K. Diffraction imageswere indexed, integrated, and scaled using...