báo cáo khoa học: " Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L ) in response to fungal pathogens and hormone treatments" ppt

... analyses of BnWRKY genes in response to fungal challengeFigure 3 Expression analyses of BnWRKY genes in response to fungal challenge. Changes in BnWRKY transcript abundance in response to (A) ... article Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L. ) in response to...

Ngày tải lên: 12/08/2014, 03:20

... (200 1) Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L. Plant Mol Biol ... 7P–R) in all plant lines, highlighting its important role in the regulation of cardosin B gene expression. Comparison of the leader intron of the cardosin B gene with pisti...

Ngày tải lên: 30/03/2014, 09:20

... Shinozaki K: Novel drought- inducible genes in the highly drought-tolerant cowpea: Cloning of cDNAs and analysis of the expression of the corresponding genes. Plant Cell Physiol 1996, 37:1073-1082. 7. ... dif- ferent levels of expression in different genotypes, and thus may be a key factor to their different levels of drought tolerance. Results Identification...

Ngày tải lên: 11/08/2014, 11:21

... form Lemma Stem Gloss AlwlAyAt Noun Al+wlAyAt Al+wlAyp Al+wlAy the states ltblgh Verb l+ tblg+h l+ >blg+h l+ blg+h to inform him Table 1: Examples of word lemmatization settings the three settings, ... Rehbein, and L. Tounsi. 2010. Statistical parsing of morphologically rich lan- guages (spmrl) what, how and whither. In Proceedings of the NAACL HLT 2010 First Workshop on...

Ngày tải lên: 20/02/2014, 05:20

... survival not only by regulation of well-known proapoptotic proteins, but also by binding proteins involved in several cellular processes involved in survival and death mechanisms in cells. Thus, 14-3-3 ... were selected and expanded. FACS analysis was also performed to ensure uniform expression of GFP in the selected cell lines, and anti-14-3-3f immunoblotting analysi...

Ngày tải lên: 06/03/2014, 22:21

... mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme. To obtain an insight into how the ... developing positive charge of what will become the oxazolinium ion intermediate (Fig. 1C). Repla- cing Asp142 by a less polarizable asparagine would reduce this electron pulli...

Ngày tải lên: 07/03/2014, 14:20

... mm NaCl) containing protease inhibitors (240 lgÆmL )1 phenyl- methanesulfonyl fluoride, 2 lgÆmL )1 aprotinin, 0.35 lgÆmL )1 bestatin, 1 lgÆmL )1 pepstatin, 2.5 lgÆmL )1 leupeptin, 0.1 6 mgÆ mL )1 benzamidin, ... Current Protocols in Molecular Biology. Greene Publishing Associates, New York. 51 Harlow E & Lane D (198 8) Antibodies – A Laboratory Manual. Cold Spr...

Ngày tải lên: 07/03/2014, 16:20

... DpnI to digest the template plasmid. Mutated plasmids were afterwards transformed into E. coli Top10 competent cells and selected on LB agar plates containing ampicillin (100 lgÆmL )1 ). Expression, ... two-step method, via incubation with lysozyme (1 mgÆmL )1 ,1hat 4 °C) and sonication. The lysate was centrifuged to remove cell debris and loaded into a column containing...

Ngày tải lên: 16/03/2014, 00:20

... °C, in 1 L of Luria–Bertani broth containing ampicillin (100 mg L )1 ). When the attenuance (D) reached 0.3 at 600 nm, the cells were induced by the addition of 0.1 m M isopropyl thio-b- D -galactoside ... NHase. Thewild-typeenzyme,aT109S mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively. The Cc2atomofaThr109 and the Cc1atomofaVal136 of the Co-...

Ngày tải lên: 16/03/2014, 16:20

... next to the unstained ovary (ov) in A. suum. (D) Section of the body wall of a female A. galli showing an intense labeling of the inner hypodermis (ihy, arrow). (E) Staining of the labyrinth of ... Trp residue, buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of retinol caused an increase of the...

Ngày tải lên: 16/03/2014, 18:20