báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L )" doc

báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc

... clusterLpPcaClone4 Oat NBS-LRR candidate from Pca clusterLpHvClone1 Barley NBS-LRR co-locating with QTL [33]LpHvClone2 Barley NBS-LRR co-locating with QTLLpHvClone3 Barley NBS-LRR co-locating with QTLLpHvClone4 ... comprehensive analysis to date of qualitative disease resistance candidate genes in perennial ryegrass. SNPs identified within candidate genes provide a valuable resource for mapping in various ryegrass ... origin.Phylogenetic analysis of perennial ryegrass R genes Phylogenetic analysis was performed based on twoselected NBS domain regions (P-Loop-GLPL and Kin-2A-GLPL). Unrelated NBS domain sequences...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... supporting information can be found in theonline version of this article.Please note: Blackwell Publishing are not responsible for the content or functionality of any supportinginformation supplied ... Mutationalanalysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase. J BiolChem 269, 11859–11868.23 Lao-Sirieix SH, Pellegrini L & Bell SD (2005) ... University of California, San Francisco.42 Wang J, Cieplak P & Kollman PA (2000) How welldoes a restrained electrostatic potential (RESP) modelperform in calculating conformational energies of organic...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... (Mana1–6(Mana1–3)(Xylb1–2)Manb1–4GlcNAcb1–4GlcNAc) wasidentiﬁed as main glycan of the vicilin-like protein fromhazelnuts [12]. The allergen Lol p 11 from ryegrass, Lolium perenne, contained MUXF as well as ... performed a Covalink-ELISA to determine IgE binding to the natural Lyc e 2 and the recombinant protein. All sera reacting with the naturalprotein in the ELISA were positive in the immunoblottingexperiments. ... recombinant Lyc e 2. SDS/PAGE analysis of electroeluted recombinant Lyc e 2 isoforms rLyc e 2.01 (lane 1) and rLyc e 2.02 (lane 2), Coomassie stain. M, molecular mass marker.1332 S. Westphal et al....

Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

... M553518kDakDa0801TH-0801THsiH-1pepcS0801TH-0801THsiH-1pepcS0801TH-0801THsiH-1pepcSALane 1 2 3 4 5 6 7 8 9 10 11 12Fig. 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 ... CtsaS190Amiceto investigate the overlap and distinct functions of Scpep1 and Ctsa.Materials and methodsCell lines and cell cultureIf not stated otherwise, the cell lines were grown in com-plete Dulbecco’s ... clinical relevance of soluble lyso-somal proteins in LSDs and a notable number of LSD-like diseases of unknown etiology, there is a com-mon interest in the identiﬁcation of the proteome of the lysosomal...

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... with collagenase (200 UÆmL)1;Gibco)inCa2+-freemedium, followed by rinsing and storage in saline mediumcontaining Ca2+ and 50 lgÆmL)1gentamicin. Stage V–VIoocytes were separated for injection ... mixture (Clon-tech). The thermal cycling parameters for RACE–PCR wereas follows: an initial denaturation at 94 °C for 30 s; ﬁve cycles of 94 °Cfor5sand72°C for 4 min; ﬁve cycles of 94 °Cfor5sand70°C ... another candidate useful for studyingthe mechanism of upregulation of functional sodiumchannels on the cell surface and increasing the rate of spontaneous ﬁring in peripheral neurons after nerve injury.It...

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... collected from farmareas in Hubei province in China. Sarcophaga falculatablowﬂy larvae, Sprague–Dawley (SD) rats, and albinoKunming mice were bred in the laboratory. E. coli strainsBL21 and ... Sarcophaga falculatablowﬂy larvae and by tail vein injection of 200 lL,subcutaneous injection of 2 mL, or intracerebroventricularÓ FEBS 2002 Molecular cloning and function of gene BmKIM (Eur. J. Biochem. ... in nerve and skeletal muscle and with lower frequency in cardiac muscle even though theincidence of cardio-pulmonary abnormalities induced bythe scorpion sting is well documented [16,17]. In...

Báo cáo khoa học: "Molecular structure and biochemical properties of lignins in relation to possible self-organization of lignin networks" pdf

... formation of molecular com-plexes by auto- and heteropolymerization in plant cell walls with an increase of ligninfunctional properties.The high frequency of relatively ... chemical and biochemical proper-ties in lignin networks. Such regularitiesmay allow self-organizing properties of lignin macromolecules, explaining theirfunctional fitness and ... in the molecular structure of lignin and mainly discussesthe biochemical heterogeneity and occur-rence of associations between lignins and other cell wall components. In...

Báo cáo khoa học: " Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine" pdf

... following profile: Activation of DNApolymerase at 95°C for 5 min, followed by 35 cycles of denaturation of DNA at 95°C for 40 sec, annealing at the50°C for 1 min, extension at 72°C for 1 min ... pathogenicity.ConclusionComplete sequence of the first Chinese porcine SaV wasdetermined and analyzed providing a gene profile of por-cine SaV presented in swine population in China today.Sequence analysis ... establishing full-lengthinfectious clones containing or not containing thisinserted fragment would now be the next step towards theidentification of this fragment involved in symptomatol-ogy and...

Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... thermalcycler, and the cycle conditions were as follows: one cycleat 95 °C for 2 min, followed by 35 cycles at 95 °C for 1 min, 56 °C for 1 min and 72 °C for 2 min, with a 10 minﬁnal extension ... withpalaeontological and biogeographic landmarks. MolBiol Evol 19, 748–761.38 Bouhin H, Charles JP, Quennedey B & Delachambre J(1992) Developmental proﬁles of epidermal mRNAsduring the pupal-adult ... vit-ripennis and A. mellifera. Additionally, there is absoluteconservation of the location, size and phase of exon 1among all RNase j genes in the available species.The above results imply that...

Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

... that a terminal Sial-lNnT unit islinked to the Hep I residue of the LPS inner core in thesialylated glycoform. The structure of the sialylated glyco-forms of the O-deacylated LPS of H. in uenzae ... strain RM118 and RM118 lgtC, Sial-Lacglycoforms were observed in addition to the higher masssialylated glycoforms. As expected Sial-Lac-containingglycoforms were not observed in the lic2A and ... killing by normal human serum [15]. In thegonococcus, LPS sialylation can occur only if an exogenoussupply of sialic acid is available [16]. In contrast, sialylatedmeningococcal LPS glycoforms...

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