báo cáo khoa học: " Nanoparticle penetration and transport in living pumpkin plants: in situ subcellular identification" ppt

... citation purposes) BMC Plant Biology Open Access Research article Nanoparticle penetration and transport in living pumpkin plants: in situ subcellular identification Eduardo Corredor 1,5 , Pilar S Testillano 1 , ... containing the dense material in an ultrathin consec- utive section. C) Detail of the region indicated in (B)(*), showing presence of nanoparticles in...

Ngày tải lên: 12/08/2014, 03:20

... (patatin, found in potato tubers) [28] and iPLA 2 b [23] – iPLA 2 c contains a consensus site for nucleotide binding and a lipase consensus motif in its C-terminal half [26]. Although the intracellular ... 2004 rate-determining factor in uncoupling protein (UCP) activ- ity [16]. Thus, t he role of mitochondrial i PLA 2 activities in regulating mitochondrial function is just now be...

Ngày tải lên: 19/02/2014, 16:20

... patterning in early embryonic development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa ¨ tzle in a lepidopteran insect, Manduca sexta, in ... variant, proSpa ¨ tzle-1B, contained a 30 bp insertion in the ORF, beginning at nucleotide 516. This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one sub...

Ngày tải lên: 06/03/2014, 09:22

... generated by starting and stopping with a 3 s interval, pulse after a 2 s interval, pulse after simultaneous injection of EDTA and Ca +2 , pulse after 8 s interval, and pulse after 4 s interval. (B) ... catalysis. These include high spin ferriheme, ferroheme and ferroheme O 2 (each with either arginine or NHA); ferriheme NO; and ferroheme NO in the presence of citrulline (as forme...

Ngày tải lên: 07/03/2014, 00:20

... shown). The following organs were regularly examined histo- logically using semithin sections: liver, lung, kidney, spleen, pancreas, retina, cornea, and spinal cord; in some instances, the inner ear ... serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) (Fig. 2B). However, two other serine protease inhibi- tors, aprotinin and antipain, had no effect, although the...

Ngày tải lên: 07/03/2014, 03:20

... enzymes will be evaluated in the following experiments. cDNAs coding for cysteine proteinases in V. · heilbornii and V. stipulata Using a PCR approach with a cysteine proteinase primer (CyPr), specifically ... manufac- turer’s instructions. Insert sequencing was carried out in both directions using T7 and SP6 primers. Inserts were se- quenced using the ABI prism BigDye TM Terminator...

Ngày tải lên: 07/03/2014, 11:20

... PAGE indicated that the PTHs were eluted as a single peak just after the PINs. Separate pools of the PTH-containing and PIN-containing crude fractions were dialyzed against de- ionized water and ... & Marion D (1998) Spatial and temporal distribution of the major isoforms of puroin- dolines (puroindoline-a and puroindoline-b) and non specific lipid transfer protein (ns-LTPe1) of...

Ngày tải lên: 07/03/2014, 12:20

... optimization, Springer-Verlag. Povey, D. and P. C. Woodland. 2002. Minimum phone error and I-smoothing for improved discriminative training. IEEE International Conference on Acoustics, Speech, and Signal ... joint expertise in natural language processing and speech recognition, and best practices in language engineering for every new domain. On the other hand, a statist...

Ngày tải lên: 08/03/2014, 02:21

Ngày tải lên: 08/03/2014, 05:21

... were incubated in NaCl/P i for 90 m in, the intracellular fluorescence signal dramatically increased in all cases (Fig. 9, Table 1). The increases, in comparison to the s ignals observed in standard ... membrane was cut in strips, corres- ponding to the different m olecular masses o f MnSOD, CuZ nSOD and actin, the last acting as an internal standard, and incubated with the corre...

Ngày tải lên: 16/03/2014, 18:20