báo cáo khoa học: " Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression" docx

... Central Page 1 of 11 (page number not for citation purposes) BMC Plant Biology Open Access Research article Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural ... related to changes in the epigenetic status of plant genomic DNA in the process of dedifferen- tiation [29] that accompanies preparation of trans...

Ngày tải lên: 12/08/2014, 03:20

... applied to MALDI-TOF-MS analysis. Acknowledgements We thank Martin Hahn and Mohamed Marahiel for the kind gift of the sulfamoyl inhibitors, Helena Jan- zen for help with statistical analysis and ... protein changes. Before the analysis, we incubated the apo- and the holo-forms of the A-PCP protein with inhibitor 1, or without any ligand. As shown in Fig. 3, in the absence of lig...

Ngày tải lên: 29/03/2014, 08:20

... levels (higher dose levels and smaller volumes) and, for each organ (i.e.: bladder and rectum) and constraint, level of compliance was above 90% (Table 5). By performing an evaluation of possible ... months (range: 12.1 to 42.2 months). The median prescribed dose was 76 Gy, (range: 68 to 78 Gy), and the mean administered dose was 76.5 Gy, with median maximum and minimum d...

Ngày tải lên: 09/08/2014, 10:21

... post-transplant medical compliance and mental health predict physical morbidity and mortality one to three years after heart transplantation. J Heart Lung Transplant 1999, 18:549-562. 9. Lewandowski ... partly induced an avoidance of social relationships, and to some extent it may have led patients to reject the social support offered to them. Correlation between social supp...

Ngày tải lên: 13/08/2014, 03:20

... sequencing up to 75% of the total length of the protein and electrospray mass analyses (mass ¼ 7504.4 Da, which is virtually identical to the calculated value) confirmed the purity and identity of the sWntx-5. Circular ... toxins display 11 residue differences and that the competition systems used (human and rat on one hand, and chicken on the other) are not identical in th...

Ngày tải lên: 21/02/2014, 03:20

... (Promega) and then normalized for b-galactosidase activity. Transient transfection and CAT reporter gene assay To analyse the expression of mitochondrial markers such as cyt c, ANT2 and b-ATPase ... as Tom40, Tom20, Tom70 and translocase of the inner membrane (TIM) such as Tim22 and Tim23 family members [40] as well as numerous chaperones such as Hsp70 [41] forming effectors...

Ngày tải lên: 23/03/2014, 15:21

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control ... co- transformants of BD-PsCBL plus AD-PsCIPK; clone 2, cotransformants of BD-PsCBL and AD vector alone; clone 3, AD-PsCIPK and BD vector alone; clone 4, cotransforman...

Ngày tải lên: 19/02/2014, 07:20

... (a 3 and b 3 ) and two heterotrimers (a 2 b 1 and a 1 b 2 ). The existence of the four types of trimer may be reflected in the broad band migrating at  200 kDa in Fig. 7. On the other hand, a band ... We observed gel shift bands in the lanes containing epitope- tagged HSF1 (Fig. 6B, lanes 5 and 8). The bands were detected at a position corresponding to the gel-shift band of he...

Ngày tải lên: 19/02/2014, 12:20

... control for the quantity and quality of cDNA. Semiquantitative analysis of RT–PCR products. Suspen- sions of Carp head kidney and liver cells were treated with 10 lgÆmL )1 LPS for 1, 3 and 6 h, individually, ... [7]. As innate immunity is known to be important in the defense of pathogens, isolation and characterization of cytokines is of prime importance. Only a few cyto...

Ngày tải lên: 20/02/2014, 02:21

... MLI, MLII and MLIII A-chains suggests that the other two genes ml2p and ml3p correspond to MLII and MLIII, respectively. Materials and methods Plant material Young leaves of mistletoe (Viscum ... separated on 2% agarose gel and the bands corresponding to genomic fragment (length of 415/430 bp) and competitor fragment (length of 388 bp) were excised. Radioactivity of th...

Ngày tải lên: 07/03/2014, 15:20