Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies ”" pptx
Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... Access “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies. ” Hajib ... al.: “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the...
Ngày tải lên: 12/08/2014, 02:20
Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... wild-type and vaccine strains. In summary, the multiplex RT-nPCR developed in this study is a highly specific and sensitive assay for the rapid detection and differentiation of wild- type and vaccine ... difficult and necessary to discriminate between wild-type and vaccine strains because the atten- uated CDV vaccine is used widely in China. So a method to specif...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo y học: "A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients" pdf
... cohorts and the sensitiv- ity and specificity of the detection methods [10-16]. The majority of mutations in imatinib-resistant patients usually occurred within the nine amino acid positions of KD including ... 9 and 22, creating a new fusion gene, BCR-ABL, with constitutive t yrosine kinase activity [2]. Targeting BCR-ABL- transfe cted cell lines and murine CML mode...
Ngày tải lên: 10/08/2014, 21:23
Báo cáo y học: "Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus" potx
... reverse transcriptase (Invitrogen) and 40 U RNase inhibitor. A total of 20 μL of the mixture was initially incubated at 25°C, then the reaction was held at 65°C for 60 min and finally it was terminated by incubation ... were found to be closely related to strains belong- ing to the Asia-1 lineage, which is distant and phylogenet- ically distinct from the vaccine stra...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo y học: "PTPN22 polymorphism and anti-cyclic citrullinated peptide antibodies in combination strongly predicts future onset of rheumatoid arthritis and has a specificity of 100% for the disease" ppsx
... from arginine into tryptophan. This amino acid change disrupts the binding of Lyp to an intracellular kinase, Csk, which can then no longer inactivate another kinase, Lck, that is involved in T-cell ... a main investigator and carried out the genotyping, the statistics and contributed to preparation of the manuscript. LÄ participated in the collection of the mate...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... real- time RT-PCR assay. Intrinsic performance of the duplex real- time RT-PCR assay Linearity Linearity of the duplex real- time RT-PCR assay was determined using serial 10-fold dilutions of a clinical sample ... B) assays in the testing of the same sample with of low load of HCV. Assay of 109 serum samples by using commercial kits The results obtained by...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo y học: " Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD" pot
... similar to the study of Laitinen et.al. S ince we ana- lyzed the immunohistochemical expression of Tn-C in the specific histological localizations of the airway mucosa instead of measuring it. F urthermore, ... was to quantify measures of remodeling in the large airways in COPD compared to smokers and nonsmokers. We therefore hypothesized that the expressio...
Ngày tải lên: 12/08/2014, 13:22
Báo cáo y học: " B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both " pot
... against TM region of HERV Envs have been successfully used in immune assays including flow cytom- etry and immunofluorescence staining by others, as they are able to detect the TM region of a ... expres- sion, thereby probably diminishing the immune reactivity and thereby also influence the pathogenesis of the actual MS attack. The role of HERV-expressing monocytes i...
Ngày tải lên: 12/08/2014, 23:22
Báo cáo y học: " G140S/Q148R and N155H mutations render HIV-2 Integrase resistant to Raltegravir whereas Y143C does not" potx
... resistance to RAL emerges rapidly both in vitro and in vivo,through the selection of mutations within the IN coding region of the pol gene, greatly reducing the susceptibility of the virus to the inhibitor. ... suggesting possibledifferencesintheresponseoftheenzymesto inhibitors and in the mechanisms by which resistance emerges. In this study, we characte...
Ngày tải lên: 13/08/2014, 01:21
Báo cáo y học: "Impaired glucose and nutrient absorption in critical illness: is gastric emptying only a piece of the puzzle" docx
... most of them fed postpylorically [7]; in 13 patients with a faeces collector because of loose stools, the caloric value of energy loss was a mean of 301 kcal/day, and 3 patients had a loss of more ... kcal/day in the stools [7]. Commentary Impaired glucose and nutrient absorption in critical illness: is gastric emptying only a piece of the puzzle? Alain Dive Depa...
Ngày tải lên: 13/08/2014, 19:20