Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps

... is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay, the truncated glycoprotein K (tgK) has good anti- genicity, also the antibody ... that the polyclonal antibody was cursorily extracted by saturated ammonium sulfate; Lane 2 stood for the purified polyclonal antibody by ion exchange column...

Ngày tải lên: 12/08/2014, 01:21

... possibly a metal- dependent enzyme. Substrate specificity analysis To study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl- D -or L -amino acids and other D -amino ... (44.8% identity and 51.2% similarity) and N-acyl- D -asparate amidohydrolase (48.5% identity and 56.5% similarity) from Alicaligenes xylosoxydans ssp. xylosoxyd...

Ngày tải lên: 08/03/2014, 16:20

... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the three ... other combinations of phosphatases and interme- diary phosphorylated enzymes. Therefore, kinetic data on the dephosphorylation of autophosphorylated ATP-cit...

Ngày tải lên: 21/02/2014, 01:21

... of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2,18]. Similarly, we observed that the steady-state amount of GroEL remains ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not have any detectable effects on the sensitivity to mitomycin C, u...

Ngày tải lên: 22/02/2014, 07:20

... except the stationary phase, the bryonolic acid level increased during the late growth phase only [16,17]. The level of isomultiflorenol synthase mRNA was high at days 16 and 20 and low at days 4 and ... b-amyrin synthase. In this study, we used the Glycyrrhiza glabra GgbAS1 b-amyrin synthase cDNA [6] to isolate cDNA for a new OSC family member from the Luffa cDNA library b...

Ngày tải lên: 08/03/2014, 23:20

... belonging to class 3 of the cystatin superfamily. They are single-chain proteins composed of an N-terminal heavy chain, the bradykinin moiety and a C-terminal light chain. The heavy chain and light chain ... Kalkkinen 1 1 Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland, 2 Norwegian College of Fishery Science, University of Tromsø...

Ngày tải lên: 08/03/2014, 23:20

... galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... mulberry seeds; the lectins are glycoproteins as they gave an orange-yellow color in the presence of phenol/ sulfuric acid. The presence of sugar in th...

Ngày tải lên: 18/03/2014, 01:20

... type of specificity. VanXY C , VanX-type and VanY-type enzymes contain most of the active site residues identified in VanX. VanXY C has 39% identity and 74% similarity to VanY in an overlap of 158 ... VanX- and VanY-type enzymes indicated that all of these enzymes contained the same Zn 2+ binding motif. VanX homologues present in the cyanobac- terium Synechocystis strain PCC...

Ngày tải lên: 18/03/2014, 01:20

... rearrangements. In the thyroid, endocytosis of thyroglobulin is the first step of the thyrotropin stimulated secretion of thyroid hormones. In the elucidation of the cellular function of the small G proteins, ... raises the possibility that the protein may be regulated by phosphorylation. In vitro mRNA regulation of p76 RBE was assessed by Northern blotting in dog t...

Ngày tải lên: 23/03/2014, 21:20

... below. AP assays All assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C. Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactate dehydrogenase reactio...

Ngày tải lên: 23/03/2014, 21:20