production purification and characterization dot blot

... Expression and characterization of the UL31 protein from duck enteritis virus Virol J 2009, 6:1 doi:10.1186/1743-422X-7-241 Cite this article as: Zhang et al.: Production, purification and characterization ... chromatography The heavy chain and light chain were approximately 55 KDa and 22 KDa, respectively B Western blot assay Lane 1, Western blotting analysis showed that a specific band was recognized by rabbit ... antibody and purify the antibody by ion exchange column chromatography Through the western blot and ELISA assay, the tgK has good antigenicity, and the antibody possesses high specificity and affinity...

Ngày tải lên: 12/08/2014, 01:21

... sequencing of our FIII-3a and FIII-3b proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles ... from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624), with a serine protease ... purification and characterization of xylanasefrom Staphylococcus sp SG-13 and its application in biobleaching of kraft pulp J Appl Microbiol 88(2):325–34 Gupta GS, Singh GP (1983) Isolation and...

Ngày tải lên: 20/06/2014, 23:20

... rD ot eud era sknaht luftraeH stnemgdelwonkcA nietorp siht fo noisserpxe ortiv ni dna ygolonhcet AND tnanibmocer fo noitacilppa eht ot gnidael ,erudecorp noitalosi ANRm na fo esu eht etatilicaf ... zedneM ,M sedlaV ,LI zelaznoG ,HD pmeK ,VR anneKcM ,AG gnidiR ,P nesdalliW 92 891-691 ,4 ,8891 yadoT lotisaraP lortnoc kcit rof snegitna 'delaecnoc' htiw noitaniccaV HD pmeK ,P nesdalliW 82 05-34S...

Ngày tải lên: 07/08/2014, 20:23

... surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15% SDS-PAGE ... subjected to SDS-PAGE and a western blotting was carried out with anti-LSV polyclonal antibody Specific blotting bands were detected at the corresponding positions (Fig.1a and 1b) Apparently molecular ... with the antibody By bleeding and separation of antiserum and DEAE cellulose column purification, anti-LSV CP IgG (ALCP) was isolated A 34.5 kDa specific blotting band was detected at the corresponding...

Ngày tải lên: 12/08/2014, 04:21

... the synthesis of fine chemicals and pharmaceuticals, paper manufacture, and production of cosmetics, and pharmaceuticals (Rubin and Dennis, 1997a,b; Kazlauskas and Bornscheuer, 1998) Lipase can ... lipid membranes and prostaglandins Microbial lipases are used to obtain PUFAs from animal and plant lipids such as menhaden oil, tuna oil, and borage oil Free PUFAs and their mono- and diglycerides ... lipase and characteristic production by Lactobacillus delbrueckii sub species bulgaricus Antonie von Leeuwenhoek 1995;67:356 – 62 El-Shafei HA, Rezkallah LA Production, purification and characterization...

Ngày tải lên: 24/03/2014, 05:20

... and use in applications We have reported here for the first time the production, purification and characterization of a novel tyrosinase from the well-known protein producer T reesei The high production ... Stereospecificity and substrate specificity The stereospecificity of T reesei TYR2 was studied by following the activities on 15 mm l-dopa, dl-dopa and d-dopa and 2.5 mm l-dopa, dl-dopa and d-tyrosine, and the ... protein band corresponding to a molecular mass of about 43 kDa AU 214 nm Biochemical characterization IEF of the purified T reesei TYR2, and subsequent staining with l-dopa, showed a band in the...

Ngày tải lên: 19/02/2014, 06:20

... environment and the gene for the enzyme was cloned and then sequenced The recombinant protein, N-D-AAase, was also produced in the Escherichia coli, purified and characterized MATERIALS AND METHODS ... New BioLabs and Gibco BRL Pfu DNA polymerase and alkaline phosphatase were from Promega and Boehringer Mannheim, respectively D-Amino acid oxidase (EC 1.4.3.3) from porcine kidney and horseradish ... amidohydrolase (44.8% identity and 51.2% similarity) and N-acyl-D-asparate amidohydrolase (48.5% identity and 56.5% similarity) from Alicaligenes xylosoxydans ssp xylosoxydans A-6 and the D-aminoacylase...

Ngày tải lên: 08/03/2014, 16:20

... Invitrogen (Paisley, UK) and New England Biolabs (Ipswich, MA, USA) Pfu Turbo and T4 DNA ligase were purchased from Invitrogen and Stratagene (Amsterdam, the Netherlands), respectively For heterologous ... strands Production and purification of ADH E coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a single colony was used to inoculate mL Luria–Bertani medium with kanamycin and ... Machielsen and J van der Oost Metals and inhibitors The effect of several salts, metals and inhibitors on the initial activity of Pf-TDH was checked using butane2,3-diol as substrate in the standard...

Ngày tải lên: 16/03/2014, 14:20

... proteins and characterized from structural and enzymatic points of view The immunoreactivity of these two proteins has also been studied Results Production, isolation, and spectroscopic and structural ... residues of the proteins, His50, Glu96 and His137 in a-sarcin and His36, Glu82, and His123 in a-sarcin D(7–22) Images were generated by the MOLMOL program [30] and subsequently rendered with MegaPov ... loaded), and (B) immunoblotting (0.1 lg protein) using an anti-Aspf1 antiserum, of Aspf1 (lane 1), and Aspf1D(7–22) (lane 2) The samples were previously reduced with 5% (v ⁄ v) 2-mercaptoethanol and...

Ngày tải lên: 16/03/2014, 19:20

... of the valence and conduction bands between the core and shell (Figure 19).14 Figure 19: Band (valence and conduction bands) alignment of different core-shell systems The first and most common ... molecular structure and established characterization 9|Page CHAPTER TECHNICAL BACKGROUND 2.1 How Quantum Dots Work? 2.1.1 Semiconductors To understand quantum dots, we must first understand the materials ... particle size and its resultant bandgap, based on the material being used and its bandgap in the bulk form (Equation 1).9 In the equation, EgQD is the theoretical bandgap of the quantum dot, Egbulk...

Ngày tải lên: 15/05/2014, 10:25

... As shown in Table I, A rubescens and L subdulcis secreted larger amounts of exocellular proteases than C geophilum and H crustuliniforme, although growth of the former fungi ... enzymes and their distribution in the soil layers References Abuzinadah R.A & Read D (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants I Utilization of peptides and ... of nitrogen and a close correlation exists were In: Infernationales 1963 Fisher, Mykorrhizazymposium Jena, Verlag, pp 123-145 Ramstedt M & S6derhall K (1983) Protease, phenoloxidase and pectinase...

Ngày tải lên: 09/08/2014, 04:20

... Engineering Modeling and Characterization of the Quantum Dot Floating Gate Flash Memory Abstract: This thesis discusses the physics, modeling and design issues of the nanoscale quantum dot flash memory ... one quantum dot floating gate are predicted successfully for the purpose of design The advantages and applicability of emerging dielectric and quantum dot materials are demonstrated and quantified ... cross-section of quantum dot memory device with uniformly spaced grids in X and Y direction The width and height of a grid are dx and dy, respectively Fig.3.4 (a) Quantum dot floating gate flash...

Ngày tải lên: 26/11/2015, 12:40

... mẫu phân tích lớn nhƣ: PCR đa mồi, lai ADN (Southern blot, dot blot, line blot) giải trình tự gen Đáng ý số phải kể đến kỹ thuật lai điểm (dot blot) - phát triển phƣơng pháp lai ADN Đây kỹ thuật ... diversity using dot- blot technology and allele-specific oligonucleotides: Maternity of hybrids and unisexual cones of hybrid origin (Lizards, Cnemidophorus)", Molecular Phylogenetics and Evolution, ... (2012), "Development and evaluation of a reverse dot blot assay for the simultaneous detection of common alpha and beta thalassemia in Chinese", Blood Cells, Molecular, and Diseases, 48, pp 86-90...

Ngày tải lên: 23/10/2016, 17:12

... between production costs and unit cost Production costs and unit cost are two different terms of production process Production costs reflect the moneytary value that company cost in process and unit ... issues as follows: • Concept of production costs and unit cost, classification and relationship between production costs and unit cost • The tenor of aggregating production costs • Estimation of ... accounting and auditing 26 Graduation thesis Aggregating production costs and evaluating unit cost Handing over finished works Making balance sheet of finished works Contract closing 1.2.2 Production and...

Ngày tải lên: 08/08/2013, 14:22

... H and Kadowaki, K (1985a) Flocculant production by Paecilomyces sp Taxonomic studies and culture conditions for production Agric Biol Chem., Vol.49, No.11, 3151-3157 - 299 - Journal of Water and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...

Ngày tải lên: 05/09/2013, 09:08

... (DMAc) firstly, and then styrene, divinylbenzene (DVB) and benzoyl peroxide (BPO) were added to the solution and stirred with water bath at 343 K The dosage of PVDF, styrene, DVB and BPO was that ... polymerization and phase inversion, and all of the other reagents were analytical grade 2.2 Preparation of the PVDF-based composite membrane The PVDF powders were basified firstly, and the double ... immersed in deionized water for 48 h, and then the sulfonated copolymer washed with deionized water and dried for usage The sulfonated copolymer with weight of 15 g and 2.5 g polyvinyl pyrrolidine...

Ngày tải lên: 05/09/2013, 16:11

... 3′ for the sense strand and 5′-GAG GAG AAG CCC GGT-insert specific sequence 3′ for the antisense strand (Table 6.1) Position X in the sense primer must complete the codon and therefore will encode ... attP and those in E coli are attB and are only 25 bp long The integration reaction involves two proteins, an integrase and a host integration factor Homologous recombination leads to the production ... sequences are slightly different and are represented as attB1 and attB2 that recombine with the attP1 and attP2 sites respectively on the donor vector, creating attL1 and attL2 sites when BP clonase...

Ngày tải lên: 25/10/2013, 22:20

... 20:1 to 80:1 Oils Chemicals and synthetics Air At low cutting speeds (40 ft/min and below), oils are highly recommended, especially in tapping, reaming, and gear and thread machining Cutting ... brass and bronze In aluminum alloys, additions up to 1-3% of zinc and magnesium improve their machinability Cutting Speeds and Feeds 354 Cutting speed is expressed in feet per minute (m/sec) and ... (ASM) Handbook;3 American Machinist's Handbook;4 Machinery's Handbook;5 American Society of Mechanical Engineering (ASME) publications; Society of Automotive Engineers (SAE) Publications; and International...

Ngày tải lên: 17/12/2013, 14:15

... achieved by band-friction cutting blades with a surface speed up to 15,000 fpm Other band tools include band filing, diamond bands, abrasive bands, spiral bands, and specialpurpose bands 33.14 ... Water, soluble oil and water, and weak solutions of sodium silicate in water are used In turning and milling plastics, diamond tools provide the best accuracy, surface finish, and uniformity of ... Grinding and abrasive machines include Surface grinders, reciprocating or rotating table Cylindrical grinders, work between centers, centerless, crankshaft, thread and gear form work, and internal and...

Ngày tải lên: 17/12/2013, 14:15

... total product and average product both fall (and marginal product is negative) Chapter Production Costs and Business Decisions 11 Price and Marginal Cost: Producing to Maximize Profits Production ... production in order to produce various goods and services Although there are thousands of different factors of production, or inputs, Chapter Production Costs and Business Decisions 10 for simplicity ... costs and benefits determine how many units will be produced and consumed up to the intersection of the marginal cost and demand (marginal benefit) curves, the marginal benefit of each Chapter Production...

Ngày tải lên: 17/12/2013, 15:19