Báo cáo y học: " Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan" pptx

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... identitywith the BAS1 protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF9 of A. thaliana.These proteins b elong to the 2Cys-Prx subfamily. A ll plan t2Cys-Prx proteins, ... were separated by HPLC and some of themwere totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2).Computer database searches based on the amino-acidsequences ... with an online phenylthiohydantoin amino-acidanalyser. F or MALDI-TOF analysis, 1 lL of tryptic digestor HPLC-puri®ed peptides was mixed with 1 lLofsaturated solution of a- cyano-4-hydroxycinnamic...

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

... polyacrylamidegels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies has shown that PAS III is largelyconcentrated ... +d+Lungc–+dNDLymph nodec–+dND a Primer pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢.bPrimer pair: 5¢-GCCATTTTAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢.cPrimer pair: ... primers Isolation of total RNA from the mammary gland of a lactating Danish Holstein cow was performed by means of an RNeasy kit (Qiagen, Hilden, Germany). Synthesis of cDNA was performed by oligo(dT)...

Báo cáo y học: " Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan" pptx

... 7:222http://www.virologyj.com/content/7/1/222Page 7 of 10RESEARC H Open Access Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, JapanRyo Hoshina1,2, ... an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The virusesdetected ... Schema of PBCV infection of the symbiotic algae of Paramecium. *Paramecium possessing Chlorella variabilis has beenreported in Japan, China, and Australia as well as the United States.Hoshina et al....

Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot

... (ARDS) and hydrostaticpulmonary oedema. Intra-alveolar MPs from ARDSpatients contain high levels of tissue factor, show anhighly procoagulant activity, and are likely contribute tointra-alveolar ... Spu-tum samples were directly spread-out in selective media,such as MacConkey agar for Pseudomonas aeruginosa and Alcaligenes xilosoxidans, manitol salt agar for Staph-ylococcus aureus, and BCSA for ... inflammatorystatus. A great advantage of the technique is that itenables sampling of the airways in a non-invasive man-ner, in contrast with other methods such as bronchialbiopsy, bronchial brushing...

Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

... theparental and mutant cells. To examine if the defect in infection was in the early stages of the life-cycle we nextexamined the extent of integration of HIV-1 DNA afterinfection of parental and ... prepared as out-lined above. 500 ng of DNA was used as a template toamplify full product by real-time quantitative PCR as out-lined above.Nuclear and Cytoplasmic separationNuclear and cytoplasmic ... bio-chemical fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... theseresidues are retained in positions corresponding exactlyto those in the A- chains of ricin and abrin [26]. Over-all, it is therefore unlikely that the A- chains differ sig-niﬁcantly in catalytic activity.The ... R,Terenzi A, Pasqualucci L, Falini B & Stirpe F (1998)Evaluation of immunotoxins containing single-chainribosome-inactivating proteins and an ati CD-22monoclonal antibody (OM-124): in vitro and ... residues involved in the active site cleft are thesame as in abrin and ricin A- chains. This suggests that the catalytic reaction is exactly the same. The sugarbinding pulchellin B-chains are 264...

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... strand 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3¢Anti-sense strand 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢Glu127Ala Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢Anti-sense ... strand 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢Asp125Ala/Glu127Ala Sense strand 5¢-GATGGTATTGATTTTGCCATAGCGCATGGTTCAACCCTG-3¢Anti-sense strand 5¢-CAGGGTTGAACCATGCGCTATGGCAAAATCAATACCATC-3¢Tyr183Phe ... 5¢-CAGGGTTGAACCATGCGCTATGGCAAAATCAATACCATC-3¢Tyr183Phe Sense strand 5¢-TATGTATGGGTTCAATTCTTTAACAATCCACCATGCCAG-3¢Anti-sense strand 5¢-CTGGCATGGTGGATTGTTAAAGAATTGAACCCATACATA-3¢Asp125Ala/Tyr183Phe This mutant was made by two consective...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... reveal anyactivity in this assay. The protein was also tested forb-cyanoalanine synthase activity by using d-cysteine and cyanide as substrates; the d-CDes protein did notshow any b-cyanoalanine ... coenzyme. Enzymes of the b-familycatalyse mainly b-replacement or b-elimination reac-tions. The d-alanine aminotransferase and the alanineracemase family are the other two independent lineages[6]. ... b-chloro-d-alanine and b-chloro-l-alanine were used in the O-acetyl-l-serine(thiol)lyase (OAS-TL) assay instead of O-acetyl-l ⁄ d-serine and the formation of cysteine wasdetermined; the d-CDes protein...

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

... 5¢-ACAAGGGTAGACCAAACACCAAGAACAGCAACAAAAGAGACGGGCGAATCACTGACCATCAACgccGTCCTGAGAGAT-3¢) and N8518 (Reverse: 5¢-TTTCACGGTTAATGCGGTGCCAGCTCCCCAACTGTAATAAATACCAGACAAATTATATGCTCCaacCCTATACGTGCCACTG-3¢); followed by secondary PCR using ... disulphideminus variant of 12F-11 incorporating mutations Cys22Ala and Cys82Val was constructed by overlapping PCR usingoligonucleotide primers N8517 (Forward: 5¢-ACAAGGGTAGACCAAACACCAAGAACAGCAACAAAAGAGACGGGCGAATCACTGACCATCAACgccGTCCTGAGAGAT-3¢) ... bondformation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys). Use of alanine and valine was initially determined...

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... carboxylase and recombinantbovine carboxylase (Table 3). In addition, the activity of mammalian carboxylases operating on nonpropeptide-con-taining substrates such as FLEEL is stimulated by ... N-terminal amino acids of the Conus carboxylase aredominated by acidic residues including three aspartateresidues and a glutamate-rich region that includes stretches of three and ﬁve glutamate ... Conus carboxylase and vertebrate vitamin K-dependent c-carboxylasesargue for conservation of a vitamin K-dependent carb-oxylase across animal species and the importance of c-carboxyglutamic acid...

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