báo cáo khoa học:" Expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis" ppsx

... leupeptin, 1-μg/ml pepstatin, 1-μg/ml aprotinin and 1% Triton X-100). The insolub le material was pelleted (15,000 × g for 15 min) at 4°C. Total protein content in the lysates was determined using ... it would be interesting to determine if NK and other NKG2D- expressing cells could also be induced to produce and secrete MICA and MICB. If the secretion of MICA and MICB by virus-...

Ngày tải lên: 10/08/2014, 10:21

... (eNOS), and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs. The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining Tissue ... multilaminar and antral follicles, but not in those of unilaminar follicles. iNOS was detected in the surface epithelium, oocytes, and theca of multilaminar and...

Ngày tải lên: 07/08/2014, 18:21

Ngày tải lên: 07/08/2014, 18:21

... (BAL and US1). d not determined. Kinetic of the expression of p24gag in supernatants of cotton rat cell clones after HIV-1 infectionFigure 2 Kinetic of the expression of p24gag in supernatants of ... cells infected with MN virus (containing ~8 ng/ml of p24gag) or from VCRT C17 cells infected with BAL virus (contain- ing ~1 ng/ml of p24gag) were collected and used...

Ngày tải lên: 12/08/2014, 04:21

... (BAL and US1). d not determined. Kinetic of the expression of p24gag in supernatants of cotton rat cell clones after HIV-1 infectionFigure 2 Kinetic of the expression of p24gag in supernatants of ... cDNA in infected cells, [3] demonstration of viral DNA integration into the genome of the cells, and [4] production of infectious viral stocks in cotton rat cel...

Ngày tải lên: 12/08/2014, 04:21

... reversed binding of the substrate, in which the leaving group, p-nitrophenol, occupies the acyl binding site and the acetyl group binds at the leaving group binding site, sterically hindering the ... enzyme and mutants [4,9–13], investiga- tions into the individual rate and binding constants underlying the kinetic properties and biocatalytic perform- ance in the synthesis and...

Ngày tải lên: 08/03/2014, 08:20

... variability in interpretation of results [23]. Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... statistics, Europe and America has the high incidence and mortality of breast cancer [1]. The incidence of breast cancer in China is 20 per 100,000 population, and the inc...

Ngày tải lên: 09/08/2014, 04:21

... lack of a wasting syn- drome in this model [17]. Both inguinal LN and spleen showed an increase of IL-2 mRNA in the acute phase of AIA (Figs 4d and 5d), similar to a significant rise of IFN-γ in ... provide information on the time course and organ distribution of the cytokine profiles. Indeed, an extensive study of longitudinal cytokine profiles in RA is complicated...

Ngày tải lên: 09/08/2014, 06:22

... fashion using a scale of 0 to 3+: (0) = no staining; (1+) = stained cells comprising >10% of the inflammatory infiltrate (2+) = stained cells comprising 30% of the inflammatory infiltrate, and (3+) = ... hematoxylin. Colon adenocarcinoma (for p53) and tonsil tissue (for Bcl-2) were used as positive controls for immunostaining. Levels of expression of p53 and Bcl-2 o...

Ngày tải lên: 11/08/2014, 23:22

... basis of ligand recognition by PABC, a highly specific peptide- binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J 23, 272–281. 9 Craig A (1998) Interaction of polyadenylate-binding protein ... lg of plasmid DNA using 10 lg of lipofectamine (Invitrogen, Burlington, Canada), according to protocols supplied by the manufac- turer. In short, the DNA w...

Ngày tải lên: 18/02/2014, 12:20