báo cáo khoa học:" Quantitative analysis of the epithelial lining architecture in radicular cysts and odontogenic keratocysts" doc

... AccessResearch Quantitative analysis of the epithelial lining architecture in radicular cysts and odontogenic keratocystsGabriel Landini*Address: Oral Pathology Unit. School of Dentistry, The University ... epithelial lining that derives from the tooth-forming organ epithelia: the so-called glands of Serres(rests of the dental lamina), the rests of Malassez (rests of the root sheath of Hertwig) and the ... quantitative analysis of the cyst lining architecture in radicular cysts (of inflammatory aetiology) and odontogenic keratocysts (thought to be developmental orneoplastic) including its 2 counterparts:...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J stepwas lowered and the subsequent ... amplitude and half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the in uence of ... fraction of the PSII cen-ters are inhibited for QBreduction by binding of aDCMU molecule in the QBpocket; the remaining PSIIcenters are unaffected. However, a saturating concen-tration of DCMU...

Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc

... process, the ENA ⁄ VASP family proteins bind to both the barbed end and sides of actin ﬁlaments [15–18]. Theseactin-binding activities are conserved in the EVH2domain [19,20]. On the other hand, the ... domainbinds to proteins containing the consensus sequenceD ⁄ E FPPPPXD ⁄ E [21–23]. The Pro-rich domain alsobinds to proﬁlin and proteins containing the SH3 and WW domains [17,24–26]. These ... that the EVH1, Pro-rich and EVH2 domains all have the potential to function in protein binding. The presentstudy revealed that the EVH2 domain is the RAD51-binding domain. Minimal RAD51 binding...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... bHLHregion, including His57 and Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for bindingto DEC1To identify the region in BMAL1 required for the binding toDEC1, ... C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identiﬁcation of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 ... together with the resultsshown in Figs 1–3, these ﬁndings indicate that the regionrequired for the suppressive activity of DEC1 is alsorequired for the interaction with BMAL1.Binding of DEC1...

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... certain Trp residues in the C-terminus of HpPex5p to the dansyl cap in the PTS1peptides, the distance between the dansyl cap in L2 and the three tryptophan residues in the model were measured. The values ... structure of the N-terminaldomain of the human homologue, solved at 2.2 A˚resolution [11]. The obtained model covers residues254–568 of HpPex5p, corresponding to the C-terminalhalf of the protein ... concludedfrom the structural model of the PTS1-bindingC-terminal domain of HpPex5p (Fig. 4). In this domainthree of the seven Trp residues of HpPex5p are found.However, of these three residues only...

Báo cáo khoa học: Structure analysis of the ﬂavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the ﬂavin reductase family pdf

... structuresdetermine the unique functions of these ﬂavin proteins? In this study, we present the crystal structure of DvMF ﬂavoredoxin and discuss the key residues forligand binding and metal ion binding, ... structure of FeR resembles the ﬂavin-binding domain of ferre-doxin : NADP+reductase [10]. The lysine residue isnot conserved; instead, an arginine residue interactswith the FAD molecule of the ... cysteine residue at or around the site corre-sponding to the zinc-binding site of ﬂavoredoxin.These lines of evidence raise questions as to why a zincion binds robustly to DvMF ﬂavoredoxin and...

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

... (twocDNAs). Initial mapping of the region of PP2Acinvolved in TIPRL binding was obtained from the cDNAs that showed positive interaction with TIPRL. The extension of these cDNAs is shown in Fig. ... puriﬁcation of threecatalytic subunits of the protein serine ⁄ threonine phos-phatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with a4 protein.Protein Expr ... recombi-nant proteins. The 3D arrangement of the binding sitesfor TIPRL and a4 on the surface of PP2Ac shows thatthey are in close proximity, but not overlapping, whichallows the assembly of the TIPRL:PP2Ac:a4...

Báo cáo khoa học: Quantitative analysis of ultrasensitive responses pot

... (1998) Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phos-phoinositide-dependent protein kinase-1 in vitro: com-parison with protein kinase B alpha. Biochem ... FEBS The response coefﬁcient In ultrasensitive responses, a small relative increase in the stimulus results in a large relative change in the response. This phrase is reﬂected in the deﬁnition of the ... if the shape of the responseunder consideration deviates from that of the Hillequation (Fig. 3A). The Hill approach is inappropriatefor the analysis of the feedback model, as the responseis...

Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... calculated using the forceﬁeld of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannoseobserved in the Man–banana lectin complex ... energyover the threshold value. The docking of MeMan into the carbohydrate-bindingsites of Orysata and other JRLs was performed with the program insightii. The lowest apparent binding energy(Ebindexpressed ... topography of the carbohydrate-binding sites of Orysata (C), Calsepa (F) and Morniga M (I). L1, L2 and L3 correspond to the loops forming the carbohydrate-binding cavity of the lectins. Strands of b-sheet...

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... initiate a bioinformatic anal-ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the twomodes of aglycone binding. The amino acid sequence of Zm-p60.1 ... aglycones in Zm-p60.1. Interest-ingly, both lysine residues and the leucine residue areconserved in all ﬁve enzymes of the group, and the alanine residue is found in all but one of the enzymes,namely ... structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type and one of the mutants. The results provide indications of the native...

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