Báo cáo y học: " Immunofluorescence Analysis of Duck plague virus gE protein on DPV-infected ducks" docx

... detection of DPV gE. In this study, we investigated the distribution of DPV gE protein on DPV-infected ducks using polyclonal antibody raised against the recombinant His -gE fusion protein by indirect ... (Table 2). Discussion The duck plague virus (DPV) gE protein is a 490-amino acid glycoprotein protein encoded by US8 gene. Figure 2 The immunogenicity of gE...

Ngày tải lên: 11/08/2014, 21:21

... The control of viral infection by tripartite motif proteins and cyclophilin A. Retrovirology 2007, 4:40. 4. Gao G, Guo X, Goff SP: Inhibition of retroviral RNA production by ZAP, a CCCH-type ... zinc-finger antiviral protein (ZAP) was originally recovered from a screen for genes conferring resistance by cells to infection by Moloney murine leuke- mia virus (MLV) [4]. In addition to...

Ngày tải lên: 12/08/2014, 23:23

... were analyzed by blue native gel electrophoresis. Statistical analysis Statistical analysis was performed by SAS (Cary, NC, USA) and Statistica (Statsoft , Hamburg, Germany). Comparisons between ... Respiratory Research 2011, 12:29 http://respiratory-research.com/content/12/1/29 Page 6 of 11 32. Davies KJ, Delsignore ME, Lin SW: Protein damage and degradation by oxygen radicals. II. M...

Ngày tải lên: 12/08/2014, 13:22

... apoptosis not by regulation of the JNK gene, but by modulating phos- phorylation of JNKs. Thus, Bag-1 may inhibit apop- tosis by suppressing the expression of Bid and Bad. Bag-1 may also enhance ... weakly basic delivery reagent, ethoxylated polyethylenimine (EPEI), as in- structed by Gene Tools. Thus, 3.7 μl of Bag-1 Mor- pho/AS stock solution (0.5 mM) and 3.7 μl of EPEI delive...

Ngày tải lên: 31/10/2012, 15:12

... variants of exon 1 of the hCDC4 gene (Figure 1A) were amplified by polymerase chain reaction (PCR). Primers were synthesized by Metabion International AG (Martinsried, Germany). Reaction conditions ... nonsense type [7-11]. Therefore, the observation that only one heterozygous mutation with no consequence on the translated protein was found while screening 14 exons of 35 pati...

Ngày tải lên: 31/10/2012, 16:49

... 54 Table 1. Canonical pathways containing thymocyte genes which changed expression levels with age. These pathways were identified by the ingenuity data analysis program (www.ingenuity.com) using ... significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte fate and decreased thymic outpu...

Ngày tải lên: 03/11/2012, 11:35

... SDS/PAGE is probably consequence of the glycosylated nature of DM64 [42]. The molecular mass of native DM64 was also analyzed using different gel filtration matrices. Upon chromatography on a Sephacryl ... sarcolemma. Typically, upon experimental intramuscular injection, these toxins induce the formation of Ôdelta lesionsÕ followed by hypercontraction of myofilaments due to increase...

Ngày tải lên: 21/02/2014, 01:21

... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transfer ... p26-alpha, demonstrating that enhanced thermotolerance conferred by these two constructs correlated with synthesis of p26 polypeptides. Additionally, when tested by centrifugation on su...

Ngày tải lên: 22/02/2014, 04:20

... as proved by the reactivity of tTG/11, tTG/7, which overlap by this region only. However, this conclusion is slightly complicated by the lack of recognition of this core element by tTG/4,/5,/6, ... R.E. (1992) Construction of solid matrix–antibody–antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal anti- body and tag-linked antigen. J. Gen. Vir...

Ngày tải lên: 08/03/2014, 09:20

... (0.07 M )ata concentration of 2 lgÆlL )1 .OnelL of the sample was then mixedwith1lLof0.5 M matrix solution of recrystal- lized 2,5-dihydroxybenzoic acid (Aldrich, Deisenhofen, Germany) in methanol containing ... spectrometer (Bruker-Franzen Analytik, Bremen, Germany) in both linear and reflection TOF configurations at an acceleration voltage of 20 kV and delayed ion extraction. The samp...

Ngày tải lên: 08/03/2014, 09:20