báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

... statement and suggests that the RNA binding site is still available and its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA ... I and group II introns frequently contain open reading frames (ORFs), which are either free-standing within the intron itself or are in- frame with the preceding 5¢ exo...

Ngày tải lên: 17/03/2014, 10:20

... decrease of the pH in the peri- apical tissues, and thus there are special demands for the sealing agent s [31]. The major task in reducing the bacterial load, concentrated in the apical region of the root ... Medicine 2011, 7:15 http://www.head-face-med.com/content/7/1/15 Page 3 of 9 RESEARC H Open Access In vitro analysis of the cytotoxicity and th...

Ngày tải lên: 11/08/2014, 20:21

... focused by repeated intramuscu- lar and intradermal injection with the HSA gene on the G1 and H1 vectors [23]. According to this and the results of others in the field some combination of immunizations, different ... drafting the manuscript. VS: participated in the design of the study, molecular cloning, cell transfec- tions and drafting the manuscript. SI: p...

Ngày tải lên: 12/08/2014, 18:22

... triangular prism binding pocket, with its amino sugar anchored in the major groove of the DNA pointing toward the 3¢-bulge-flanking base pair. We were interested in small molecules that can selec- tively bind ... overhanging nucleotides were excised completely from the 3¢-terminus in the anneal- ing oligomer, and consequently, no expanded band was observed during incubation...

Ngày tải lên: 07/03/2014, 06:20

... hpf embryos were incubated during 15 min with different kinase inhibitors at 30 °C and then used to analyse in vitro recom- binant CNBP phosphorylation in kinase buffer containing 100 lm ATP and 5 lCi ... affects the properties of a variety of proteins with common biochemical activities, such as the binding to single-stranded nucleic acids and the remodelling of their...

Ngày tải lên: 16/03/2014, 12:20

... [22–25]. Since a functioning zinc-bind- ing domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the oligomeriza- tion state of C101-3FLAG. Therefore, the molecular mass ... within 27 Da of the calculated molecular mass. Finally the same line was used to predict the molecular mass of a third FLAG-reactive protein between alpha and c-3FL...

Ngày tải lên: 16/03/2014, 23:20

... pair of A domains and PCPs and catalyze the formation of the peptide bond between the upstream amino acyl or peptidyl moiety tethered to the phosphopantetheinyl group and the free amino group of the ... The structural and functional roles of the residues involved in the substrate selection and binding are proposed through the analysis of the predi...

Ngày tải lên: 23/03/2014, 13:20

... for the first time that the self-cleavage of SPase I is happening within the S. pneumoniae cells in all the growth phases, and the cells maintain the active SPase I at the highest level in the ... study, we were interested in investigating the effect of phospholipid on the self-cleavage of S. pneumoniae SPase I. In the pres- ence of phospholipid, the...

Ngày tải lên: 23/03/2014, 21:21

... fusion protein immediately after the arginine of the new epitope, and removed the fragment of the PRAI (ba) 8 -barrel corresponding to a 6 (ba) 7)8 . The remaining fragment of PRAI consisted of 130 ... importantly, the residues contributing to one of the hydrogen bonds in the core of the barrel, Lys258 (b1) and Gln332 (b4), remain in trPRAI. It is temptin...

Ngày tải lên: 30/03/2014, 20:20

... more fully, the properties of PTOX in vitro. In this report, we produced the protein in E. coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable ... probably the consequence of the increase in the overall quinone reducing activity of the membranes and not a direct effect on PTOX activity. This is co...

Ngày tải lên: 31/03/2014, 07:20