... sequences and two reporter genes, HIS3 and lacZ, was initially transformed with the bait plasmid pHybLex/NUB1. The resulting transformant was used as a host for the following transformation with the prey ... generated DNA probes from the same synthetic oligonucleotide as described in the Northern Blot Analysis section for UbC1. The probes were labeled with [ 35 S]dATP[...
Ngày tải lên: 23/03/2014, 12:20
... GA: Globalization and health: results and options. Bul- letin of the World Health Organization 2001, 79:834-841. 37. Yach D, Bettcher D: The globalization of public health, I: threats and opportunities. ... globalization of TFCs and the foods they promote; the globalization of advertising/marketing agencies; and the globalization of communication tech- nol...
Ngày tải lên: 11/08/2014, 18:20
Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-affinity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx
... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the full line is the fit of a one-site ... laser line was used for excitation, and emission was detected between 520 and 550 nm for YFP fluorescence, between 510 and 535 nm for GFP fluores- cence, and bet...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: N-glycosylation influences the structure and self-association abilities of recombinant nucleolin pot
... or none of the potentially N-glycosylable sites of nucleolin. These recombinant glycoproteins were produced and charac- terized by MALDI-TOF and GC ⁄ MS, CD and fluo- rimetry, and their self-interaction ... correct folding of the recombinant pro- teins, and the influence of N-glycosylation site occu- pancy in the structure of the proteins. SPR and PAGE studies confirm the...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx
... discerned from the native and mutant crystal structures. Effects on hydrogen bonding networks may change the electron density distribution around and in the active site and, together with small conformational ... stability The VsEndA variants Q69N and K71N and the VcEndA variants N69Q and N71K were expressed in a soluble form at levels comparable with the native endon...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... iden- tified as the major and the minor forms of the holo- ACP structure, on the basis of their percentage contri- butions (65% and 35%, respectively) to the overall structure of the protein. The structures ... dependence of the C m value (Fig. 4A), but the m value is independent of temperature, as per the expectations of LEM (Fig. 4C). The mean m values for the a...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: "Learning How to Conjugate the Romanian Verb. Rules for Regular and Partially Irregular Verbs" docx
... alternation: a→ea for the 3rd person singular and plural, t→t¸ for the 2nd person singular; ”a des¸tepta” (to awake/arouse); 13. alternation: e→ea for the 3rd person singular and plural, t→t¸ for the 2nd ... dataset. These, in order, encode the features. The verb (to) walk is therefore encoded as a row vector with ones in the columns corresponding to the features w...
Ngày tải lên: 17/03/2014, 22:20
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... Health, Labor and Welfare of Japan, a grant from the Japanese Health Sciences Foundation, and a grant for research fellow- ships from the Japanese Society for the Promotion of Science for Young Scientists. ... grant-in-aid from the Third-Term Comprehensive 10-Year Strategy for Cancer Control, health science research grants for Research on the Human Genome a...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học nông nghiệp " Introduction of the principles of GAP for citrus through implementation of citrus IPM using Farmer Field Schools - Milestone 9 Project Validation and Impact Assessment Report Part 1" ppt
... the farm gate between 2 and 12,000 VND per kilogram, for mandarins the price can go over 20,000 VND per kilogram and for pomelo from 3-7,000 per kg for Nam Roi variety in the Mekong delta and ... Centre 4 and trainers from Nghe An and Ha Tay provinces. The workshop in My Tho was attended by staff from the Southern Regional Plant Protection Centre and trai...
Ngày tải lên: 21/06/2014, 05:20
Báo cáo khoa học: "Granulosa cell tumor of the ovary and antecedent of adjuvant tamoxifen use for breast cancer" pot
... that the y have no competing interests. Authors’ contributions HA was responsible for the conception and design for the manuscript, the clinical work, the search for the literature, and the editing ... probable that the association of granulosa cell tumor and the use of tamoxifen for breast cancer is just a random observation and there is no relationship betw...
Ngày tải lên: 09/08/2014, 03:22