... 33 CBPs bind to a broad selection of< /b> insoluble carbohydrates (e.g. ChbB, which binds both a- and < /b> b- chitin [21], and < /b> Chb3 from St. coelicolor,which binds a- chitin, b- chitin, colloidal chitin and< /b> chitosan ... 5¢-GGATGAGCTCTATACTCACATCTTGAGC-3¢; reverse primer, 5¢-TTGTGGGCCCAACCAATCTATGAAGAATT-3¢). The PCR product was cloned using thezero blunt TOPO-cloning kit (Invitrogen, Carlsbad, CA,USA). The ... instructions, containingends compatible with the expression vector (forwardprimer, 5¢-GGTATTGAGGGTCGCCATGGTTATGTTCAATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAGAGCCTTACAAGAAGGGTCCAAAGA-3¢). The PCRproduct...