báo cáo khoa học: ""I washed and fed my mother before going to school": Understanding the psychosocial well-being of children providing chronic care for adults affected by HIV/AIDS in Western Kenya" pdf
... to be critical in coping and promoting psychosocial well-being [42,43]. In line with Antonovsky's salutogenic approach and to broaden our understandings of the psychosocial well- being of ... affected by HIV/AIDS. In the absence of working adults in their households, many of the children were responsible for the generation of food and...
Ngày tải lên: 11/08/2014, 14:21
... footnote) in the reaction mixture may reduce a proportion of the FMN domain, converting some of the enzyme to the active form. The 3-fold increase in activity may arise from the binding of the FMN ... addition of 1 mm homocysteine, and then further incubated at 37 ° C for 10 min. The reaction was quenched at 90 °C for 3 min, and the reaction mixture w...
Ngày tải lên: 18/02/2014, 08:20
... including creating it, storing it, editing it, com- paring it with other stored models, finding it again in a principled way, visualizing it, sharing it, running it, analysing the results of the ... many and they act in nonlinear ways). By contrast, making mathematical models of the biologi- cal systems one is investigating (and seeing how they perform in silico) is genera...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... 90 s, followed by acetone for 1 min, before rinsing in distilled water. The slides were then stained for 5 min in a toluidin blue solution (0.1% toluidin blue, 1% borax; pH 11), rinsed with distilled water ... nt250436–253041) to obtain pJL265. This fragment includes exon 0 of phk-2, the first intron, and the first exon to the ATG, which is used to initiate transla...
Ngày tải lên: 08/03/2014, 08:20
báo cáo khoa học: " LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus?" ppsx
... Bovine Serum, 100 units/ml of penicillin and 100 μg/ml of streptomycin. Cytospins of the OE-33 cell line were fixed in acetone and dried for 10 minutes. Rehydration, blocking, and the staining ... in intestinal metaplasia and EACs may thus sustain multi-step carcinogenesis. Our findings seem to be very well in line with current under- standing of carcinogenesis a...
Ngày tải lên: 10/08/2014, 10:21
báo cáo khoa học: " Fever, hyperglycaemia and swallowing dysfunction management in acute stroke: A cluster randomised controlled trial of knowledge transfer" pot
... patient performance in 10 activities of daily life. The items are divided into groups that relate to self -care (feeding, grooming, bathing, dressing, bowel and bladder care, and toilet use) and a ... those ASUs randomised to the intervention group. Following a minimum period of three months to allow the FeSS intervention to become an integral part of usual clini...
Ngày tải lên: 11/08/2014, 16:20
Báo cáo khoa học: " A simple and rapid Hepatitis A Virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations" potx
... substitution in the 2B protein was used as the background to construct HAV-Bsd. The blasticidin resistance (Bsd) gene coding for the blasticidin deaminase was inserted into the 2A-2B junction of the infectious ... KK and MLVT par- ticipated in the design of the study. MYY and GGK con- ceived of the study, and participated in its design and coordinatio...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc
... naturally occuring FtGLDH AA and the artificial AtGLDH1 AA , are indeed exclusively due to the insertion of two additional alanyl residues into the N-terminus of the H-protein. In conclusion, the substrate ... method for producing recombinant H-proteins leaves a small extension of four amino acid residues at the N-terminus of each recombinant protein after removal...
Ngày tải lên: 23/03/2014, 04:20
Báo cáo khoa học: New insights into Fragile X syndrome Relating genotype to phenotype at the molecular level docx
... analysis led to the suggestion that dFXRP is the ancestral pro- genitor of the vertebrate FMRP and FXR proteins, and that in flies this single protein performs the func- tions carried out by the entire ... be to disrupt a ‘hydrophobic platform’ involved in RNA recognition. They had observed such a mode of KH domain–RNA interaction in the cocrys- tal structure o...
Ngày tải lên: 30/03/2014, 15:20
Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... cloned into pDG148 vector to obtain the plasmid pOpac (Table 1). The respective sip(ba) genes were isolated from initial cloning vectors and cloned into the vector pQE16 in frame with the His-tag-encoding sequence. ... 0.5, and then divided into cultures A and B, each of 5 mL. After i ncubation for 10 min a t either 3 0 °Cor42°C, the t wo cultures were labelled for 1...
Ngày tải lên: 21/02/2014, 03:20