báo cáo khoa học: " Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET" pot

... 2:70 http://genomemedicine.com/content/2/9/70 Page 5 of 13 MET H O D Open Access Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET Yoshiyuki Shibata † , Ankit Malhotra † , Anindya Dutta * Abstract Anchored ... al.: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET. Genome Medicine 2010...

Ngày tải lên: 11/08/2014, 12:21

... risk for hereditary colorectal cancer. The Journal of Laboratory and Clinical Medicine 2004, 143:59-66. 8. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of ... 10 ug of total cell protein. (C) Detection of MLH1 and MSH2 proteins on western blots with a mixture of varying amounts of HCT116 and LoVo cell lysates. Results show that the...

Ngày tải lên: 10/08/2014, 10:21

... empirically by data permutation as described by Churchill and Doerge [6] for the line-cross analyses and by simulating the data assuming a polygenic infinitesimal model and a normal distribution of performance ... were very similar except for SSC 13. A QTL explaining 1 to 3% of the phenotypic variance of body weights from 10 to 17 weeks of age was located at theend of the q...

Ngày tải lên: 09/08/2014, 18:21

... characterized by the dissolution of epithelial cell–cell junctions and reorga- nization of the actin cytoskeleton with the formation of focal adhesions and stress fibers, acquisition of a spindle-shaped ... targeted for disposal go to only one of the two daughter cells, leaving the other relatively clear of such signaling byproducts. A deeper understanding of the role of...

Ngày tải lên: 07/03/2014, 01:20

... homogeneity of isochore can be defined by an index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome. If h ( 1, the variance of GC content of the ... insertion in the Columbia ecotype was con- firmed by PCR amplification across the junctions of mitochondrial and unique nuclear DNA, followed by the sequencing of t...

Ngày tải lên: 30/03/2014, 20:20

... event in the repair of DNA damage and the induction of DNA degradation leading to cell death; therefore, the phos- phorylated form of H2AX (cH2AX) is a sensitive marker for DNA double-stranded ... fragmentation, which is one of the most typical characteristics of apoptosis. Regulators of DNA fragmentation One of the terminal processes of apoptosis is DNA degradatio...

Ngày tải lên: 14/02/2014, 22:20

... Purification step of C. cinereus DNA polymerase a. One unit (1 U) of DNA polymerase was defined as the amount needs to catalyze the incorporation of 1 pmol of [ 3 H]-d TTP into a DNA polymer in 30 ... despite the lack of bulk DNA synthesis, we observed significant levels of expression of p140 and p48 in both the leptotene and zygotene stages (Fig. 2). In both cases, the hig...

Ngày tải lên: 20/02/2014, 11:20

... the number of homophone problems for a homophone set. We solve them by the orig- inal decision list DLO. Let G (or H) be the ratio of the number of homophone problems by judged by a (or f~ ... method used for the homophone problem is trivial if the method is evaluated by the precision of distinction using the following for- mula: number of correct discriminations...

Ngày tải lên: 22/02/2014, 03:20

... intensity of the essDNA–Mhr1 complexes. For analysis of Mg 2+ depen- dency, Mhr1–essDNA complexes were formed by addition of Mhr1 to 13 lm essDNA in 25 mm Tris ⁄ HCl (pH 7.5) in the presence of 10 ... a temperature-regulated holder) for 1 min. The excitation wavelength was set at 295 nm for selective excitation of Trp residues, or at 320 nm for excitation of essDNA. For...

Ngày tải lên: 06/03/2014, 09:22

... loading of the ring shaped DnaB helicase. Indeed, ATP hydrolysis by DnaC is not required for DnaB loading as this reaction can be performed by ATP–DnaC, ADP– DnaC or even nucleotide free forms of DnaC ... affinity of DnaC for single stranded DNA. In contrast ADP– DnaC does not inhibit DnaB helicase and has lower DNA binding affinity. Thus, it has been proposed that ATP–DnaC int...

Ngày tải lên: 07/03/2014, 21:20