báo cáo khoa học: " Models of the human metabolic network: aiming to reconcile metabolomics and genomics" pps

... [3]), and pre- su mably ultimately in humans, it will be important to be able to predict and monitor the metabolic consequences of these genetic manipulations, thus bringing together the two ... metabolism with the seemingly ‘parameter-free’ genomic description, and vice versa. © 2010 BioMed Central Ltd Models of the human metabolic network: aiming to r...

Ngày tải lên: 11/08/2014, 12:20

... no tryptophan residue, and therefore, the fluorescence of these mutants corresponded to that of the inserted trypto- phan residue. The fluorescence peaked at 341, 343 and 347 nm for the tryptophan ... per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig. 5A...

Ngày tải lên: 19/02/2014, 06:20

... receptor and may play a role in memory retention and retrieval [15,16]. On the other hand, we and others reported that A-LAP ⁄ ERAP1 is a final processing enzyme of the precursors of the major histocompatibility ... gene. Isolation and characterization of human L-RAP gene promoter Figure 2 shows the nucleotide sequence of the 5¢-flank- ing region of the human L...

Ngày tải lên: 23/03/2014, 13:20

... [29]. The function of b-arrestin is to uncouple the receptor f rom the G-protein, thereby mediating recepto r desensitization, and furthermore to couple the receptor to the endocytic machinery and ... radioligand internalization to assay receptor internalization is based on the assumption that receptor and ligand are endocytosed together and that the intracellu...

Ngày tải lên: 30/03/2014, 15:20

... fibroin of the silkworm, Bombyx mori, is synthesized in the posterior silk gland (PSG) cells, secreted into the lumen of PSG, associated with sericin in the lumen of the middle silk gland (MSG) and ... contributed to the restoration of the high-level secretion of fibroin. In the two transgenic lines (L6 · 7 and L7-4), fhx/P25 was detected as two bands of 30 an...

Ngày tải lên: 16/03/2014, 16:20

... I-937 of P. cinnabarinus and 145 mgÆL )1 from the derived monokaryotic strain ss3 of P. cinnabarinus. In order to improve the secretion of the recombinant laccase, the laccase cDNA was fused to the ... vector are indicated the name of the primers u sed for amplification of the laccase cDNA and addition of cloning sites, recipient Aspergillus expression vecto...

Ngày tải lên: 17/03/2014, 11:20

... P47. Effect of the PsbH absence on the accessibility of the D1 protein to trypsin The e ffect of the PsbH absence on the structure of the PSII core complex was further probed by trypsinization of the D1 ... of CP47 to the heterodimer D1±D2 as well as on the bicarbonate binding to the acceptor side of PSII. Never- theless, there is also recent report s...

Ngày tải lên: 17/03/2014, 11:20

... drugs from the cytoplasmic leaflet of the membrane to the aqueous medium rather than to the extracellular leaflet [12,13]. The question remains as to whether the release of drugs from the cytoplasmic ... mem- brane into the cytoplasm occurs at a rate similar to that of the flip-flop of dye across the plasma mem- brane. In the case where the release into th...

Ngày tải lên: 29/03/2014, 08:20

... M r,analyte and M r,ligand are the relative molecular mass of the analyte and ligand, respectively; n is the number of analyte-binding sites on the protein; (A) is the fraction of active sites on the ... plot of the equilibrium-binding isotherms obtained in the absence of ATP ⁄ Ca 2+ (d), in the presence of 500 lM ATP (.) and in the pres- ence of 500 l...

Ngày tải lên: 30/03/2014, 11:20

... monitoring of therapy is needed for human studies. The tracking of viral delivery could give clinicians the abil ity to correlate efficacy and therapy and monitor potential viral toxicity. Furthermore, ... procedures, and regulatory requirements of the Institutional Animal Care and Use Committee, the Research Animal Resource Center of MSKCC, and the National In...

Ngày tải lên: 18/06/2014, 19:20