báo cáo khoa học: " Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization" pptx

... result of this approach indicated the orienta- tion of 5-methyltetrahydrofolate in the MetS active site. Figure 5 shows the superimposed structures of DHPS and 5-methyltetrahydrofolate. The model of ... a molecular model of the methyltetra- hydrofolate-binding domain of the rat liver MetS was constructed. In the absence of a high-resolution struc- ture of the methyltetrahydrof...

Ngày tải lên: 19/02/2014, 05:20

... module of Cel7B from H. jecorina have generated conflicting results about the extent and localization of glycosylation of this protein [8,16,18]. The present detailed structural study of Cel7B cat expressed ... 2). Comparative MS analysis of the intact protein before and after Endo H treatment confirms that it consists of a mixture of glycoforms, which vary in the extent of both...

Ngày tải lên: 19/02/2014, 12:20

... amounts of normalized messenger RNA were plotted as a function of time and the slope of this curve was used to calculate the interval period of time within which half of the original amount of mRNA ... expression profile of the Cyr61/CCN1 gene to that of the CTGF/CCN2 gene and showed that S1P coordinately regulates the expression of both Cyr61/ CCN1 and CTGF/CCN2 but the...

Ngày tải lên: 08/03/2014, 08:20

... gene switch. This gene switch showed negligible levels of background reporter gene activity in the absence of ligand and the highest levels of induced reporter gene activity in the presence of a ... in terms of relative light units (RLU) per microgram of protein. Data shown are the average of three replicates ± standard deviation. A. K. Singh et al. Improvement of ecdy...

Ngày tải lên: 15/03/2014, 23:20

... compilation ª 2008 FEBS 6029 of EPEC infection show alterations of TJ ultrastruc- ture (Fig. 5B–G) characterized by the loss of microvilli at sites of attachment, intimate attachment of bacteria to the ... role of lipid rafts in EPEC invasion of epithelial cells, we used confocal fluores- cence microscopy to observe the entry of the bacteria into epithelial cells, and confirme...

Ngày tải lên: 16/03/2014, 04:20

... upon addition of BNF, and that this interaction did not occur when carbaryl, instead of BNF, was added to the medium. Discussion The molecular mechanisms underlying the activation of genes under ... concentra- tion of h 2 . Noncompetitive, reversible inhibition This model postulates the binding of h 1 and h 2 to two inde- pendent binding sites in the receptor, and that binding of...

Ngày tải lên: 16/03/2014, 10:20

... uptake of precursors from the growth medium. In addition, the involvement of nucleotides in regulatory processes such as regulation of gene expression and modulation of kinetic properties of enzymes ... 2 mL of 0.9% (w/v) NaCl. Fifty milliliters of SA medium with glucose and erythromycin was inoculated to D 436 of 0.025 and the growth monitored by measuring D 436 .AtD 436 o...

Ngày tải lên: 16/03/2014, 16:20

... methods for suppressing gene expression, they have a number of limitations; a useful review can be found elsewhere [18]. The DNAzyme technique offers a new way of suppressing gene expression. In ... Inhibition of uPAR by DNAzymes FEBS Journal 272 (2005) 3572–3582 ª 2005 FEBS 3575 all of which contribute to focal proteolysis and regu- lation of wound healing, angiogenesis, and me...

Ngày tải lên: 16/03/2014, 22:20

... sequencing of the Psb H protein, Ms. Jana Hofhanzlova  forableassistanceandProf.J.Ma  lek for critical reading of the manuscript. We are grateful to Prof. J. Barber for a kind gift of the IC7 ... accessibility of the D1 protein to trypsin The e ffect of the PsbH absence on the structure of the PSII core complex was further probed by trypsinization of the D1 protein in isolated...

Ngày tải lên: 17/03/2014, 11:20

... highly polymorphic Ser2 gene of Bombyx mori. Gene 86, 177–184. 20 Garel A, Deleage G & Prudhomme JC (1997) Structure and organization of the Bombyx mori sericin 1 gene and of the sericins 1 deduced ... numbers of mRNAs of L-chain and H-chains of the recombi- nant IgG, and sericin-1 per 10 ng of total RNA. The indicated values are 10 )5 of the actual copy numbers. b D...

Ngày tải lên: 23/03/2014, 04:20