báo cáo khoa học: " Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey" pps

... as: Hsu et al.: Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey. BMC Plant Biology 2011 11:60.Submit your ... proteinsencoded in these genes was also determined in this sys-tem. With this combination of approaches, 5 novel Onci-dium gene promoters of genes abundantly expressed in flowers were cloned and ... response pro-tein (OnDRRP) was expressed in fully blooming flowers and Expansin (OnExpansin) was highly expressed in thelip (labellum) extending stage. The 3 trypsin inhibitor genes were expressed...

$Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf$

Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf

... PDZdomains. In the presence of IKEPP, the EC50 of GC-C for ST increased 10-fold, suggesting that IKEPP regulatesligand-mediated activation of GC-C. Interestingly, IKEPPis expressed in T84 and ... with NaCl/Pi and ﬁxed in NaCl/Picontaining 4% paraformaldehyde for 20–30 min. Cells were washed and incubated with 2%bovine serum albumin and 0.1% Triton X-100 in NaCl/Pi for 1 h at room ... cyclase domain of the 145 kDa form uponligand binding leads to a conformational change, exposing asignal that promotes internalization and exposure of aubiquitination signal, leading to selective...

Báo cáo khoa học: Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY are novel substrates of fibroblast activation protein-a pdf

... of natural substrates is poorly characterized. Identifying substrates is a crucial step in gaininginsights into the precise functions of proteases and their mechanisms of action in biology and ... throughoutthe brain, and is involved in the regulation of energybalance by stimulating increased food intake. Pep-tide YY (PYY) is produced by the gastrointestinaltract, and, via NPY receptor binding, ... undecapeptide hormone belongingto the tachykinin family and is released during the acti-vation of sensory nerves, causing vasodilation, oedema and pain through activation of neurokinin 1 receptors.Substance...

Báo cáo khoa học: IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain pdf

... 2006)doi:10.1111/j.1742-4658.2006.05556.xRepression of poly(A)-binding protein (PABP) mRNA translation involvesthe formation of a heterotrimeric ribonucleoprotein complex by the binding of PABP, insulin-like growth factor II mRNA binding ... three out of its four RNA-binding domains, whereas KH III-IV domain of IMP1 isnecessary and sufﬁcient for binding to the ARS. In addition, the strongestbinding site for both PABP and IMP1 on ... wasABCFig. 5. Binding afﬁnity of the ARS RNA to PABP and IMP1 (A) and (B) Gel-shift assays of binding of PABP and IMP1 to the ARS RNA.Uniformly radiolabelled ARS RNA was incubated with an increasingamount...

Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

... efficient in harvesting bacterial DNA and reducing the remaining PCR inhibitors. Therefore, the QIAamp DNA Mini Kit was utilized for improved efficacy of the multiplex real-time PCR assay in this ... characterisation of Salmonella flagellin genes. Acta Vet Hung 2005, 53, 163-172.14. International Organization for Standardization (ISO). Microbiology of food and animal feeding stuffs. Horizontal ... Twenty-five g of meat samples (beef and pork) were homogenized with 225 ml of BPW in a BagMixer (Interscience, France) for 2 min to prepare artificially inoculated samples. Nine ml of the homogenized...

Báo cáo khoa học: "A new data processing system for root growth and ramification analysis: description of methods" pot

... deleted; the coordinates of branch point and of final point of virtualsegments; and 5) the rank of branch point.Final data structure: resorting root seg-ments In order to ... segment is definedby the Cartesian coordinates of somecharacteristic points (e.g., terminal points(initial and final) and branching pointswhere the laterals of the order ... inserted; a running index which is thenumber of the root to which the segmentbelongs; the root order; the number of branching points; a following link indexspecifying...

báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs" docx

... set and used onehalf for training the classifier and the other half for testing.To obtain a stable informational value, this partitioning,training, and testing was performed in terms of 500 ... upon RNAi of Mlo, thus providing proof of concept for its usefulness in detecting gene-target effects.Conclusion: Besides saving labor and enabling a screening of thousands of candidate genes, ... susceptibility indices according to Equation(1) for each scanned slide in terms of the number of infected transformed cells and the total number of trans-formed cells. The detailed description of necessary...

Tài liệu Báo cáo khoa học: Stem–loop oligonucleotides as tools for labelling double-stranded DNA pdf

... dephos-phorylated by adding shrimp alcaline phosphatase (USB,Cleveland, OH, USA) (1 U for XmnI, 12 U for DraIII,2.5 U for XbaI) and incubating for 2 h at 37 °C. Phospha-tase was inactivated for 20 min at 65 ... to investigate thefeatures of this type of complex. Efﬁcient complex for- mation was observed using both the pyrimidine and purine motif triple helices. In particular, the in uence of heating ... sensitivity and speciﬁcity of this approach. Heating the samples isnecessary for efﬁcient complex formation. Stem length and heating conditions have a great in uence on thelabelling yield for both GT and...

Tài liệu Báo cáo khoa học: "A Uniﬁed Syntactic Model for Parsing Fluent and Disﬂuent Speech∗" ppt

... repair sym-bols3, and interruption point information. While anintegrated system for processing and parsing speechmay use both acoustic and syntactic information toﬁnd repairs, and thus may have ... method for pars-ing speech that contains speech repairs. This systemachieves high accuracy in both parsing and detectingreparanda in text, by making use of transformationsthat create incomplete ... annotations in directories 2 and 3 for training, and the ﬁlessw4004.mrg to sw4153.mrg in directory 4 for evalu-ation, following Johnson and Charniak(2004).The input to the system consists of the terminalsymbols...

Báo cáo khoa học: "A Morphologically Sensitive Clustering Algorithm for Identifying Arabic Roots" docx

... is suffixing?Journal of the American Society for InformationScience, 42/1, pp 7-15.Hmeidi, I., Kanaan, G. and M. Evens (1997) Design and Implementation of Automatic Indexing for Information ... contributing stronger evidence.We conducted a series of experiments withdifferential weightings, and determinedempirically that 0.25 weight for stringscontaining weak letters, and 0.50 for stringscontaining ... treatment of infixes and accurate clustering to up to 94.06% for unedited Arabic text samples, without theuse of dictionaries.IntroductionCanonisation of words for indexing is animportant and difficult...

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