báo cáo khoa học: " Macromolecular composition of phloem exudate from white lupin (Lupinus albus L )" pptx

... et al.: Macromolecular composition of phloem exudate from white lupin (Lupinus albus L. ). BMC Plant Biology 2011 11:36. Submit your next manuscript to BioMed Central and take full advantage of: ... technical replicates each. Figure 7 Accumulation of miR399 in L. albus phloem exudate in response to Pi deficiency. Northern blot of 5 μg small RNA extracted fr...

Ngày tải lên: 11/08/2014, 11:21

... regulation of the decarboxylase system. Indeed, the presence of this small subunit distinguishes the 4-Hpd from all other groups of glycyl radical enzymes. While several hundreds of putative glycyl ... cells were grown aerobically in LB medium supplemented with glucose (0.2%), carbenicil- lin (100 lgÆmL )1 ) and chloramphenicol (50 lgÆmL )1 )at 28–30 °C (pET-DX4) and 37 °C (pET-X1)....

Ngày tải lên: 07/03/2014, 15:20

... dog red blood cells. J. Gen. Physiol. 1995, 105, 677-699. Parker JC, Gitelman HJ, Glosson PS, Leonard DL: Role of calcium in volume regulation by dog red blood cells. J. Gen. Physiol. 1975, 65, ... intracellular con- centration of Cl - in red cells from low-K + species. Using a buffered physiological me- dium containing 150 mM Cl - for suspension of ferret red cells and 36 Cl as trac...

Ngày tải lên: 12/08/2014, 15:20

... (Table 3), with genomic DNA from V. salmonicida as a template. In a 0.2-mL PCR tube, a total of 50 lL reaction mix containing 37.5 lL water, 5 lL10· ThermoPol reaction buffer, 3 lL 25 mm MgCl 2 ,1lL10mm ... extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila. Gene 122, 175–180. 9 Focareta T & Manning PA (1987) Extracellular proteins of Vibrio cholerae: molecular clon...

Ngày tải lên: 19/02/2014, 05:20

... a transient. In excitable systems, stochastic oscillations often take the form of stochastic limit cycles, in which the oscillations follow a relatively well-defined path, leading to a ring-like structure ... the effect of diffusible synchronizing agents on these oscillations. If they can be synchronized between cells, this might yield robust multicellular oscillators which again would cha...

Ngày tải lên: 19/02/2014, 07:20

... by ½P f ¼½P t ÀfðDA=DA 1 Þ L t gð2Þ From eqn (1) it is clear that the X-intercept of a plot of log[DA/(A c ) A 1 )] vs. log[P] f will yield pK a for t he lectin– porphyrin association. A representative plot of log[DA/ (A c ) ... previously [27–31]. All other reagents were obtained f rom local suppliers and w ere of t he highest purity available. Purification of MCL MCL was purifi...

Ngày tải lên: 19/02/2014, 16:20

... value, we conclude that the system can be used as a tool that helps us compile a glossary. Second, we tried to examine the recall of the system. It is impossible to calculate the actual re- call ... 15–22. Table 1: Result for “natural language processing” candidate Tech. Rel. 自然言語処理 (natural langauge pro- cessing; NLP) 自然言語処理技術 (NLP technology) √√ 自然言語処理システム (NLP s...

Ngày tải lên: 20/02/2014, 16:20

... hard enough; finding all of them reliably is well beyond the scope of this work. Finally, any system applied to real input, no matter how carefully designed, will occasionally make errors in ... automatically generating a full-scale dictionary is the ability to detect many more types of SFs. SFs involving certain prepositional phrases are particularly chal: lenging. For example,...

Ngày tải lên: 20/02/2014, 21:20

... - Additionally allowed 16 - Generously allowed 0 - Disallowed 1 (Val167) - Structure of flavoredoxin N. Shibata et al. 4842 FEBS Journal 276 (2009) 4840–4853 ª 2009 The Authors Journal compilation ... McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R et al. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948. Structure of flavoredoxin N. Shibata et al. 4852 FEBS Jour...

Ngày tải lên: 07/03/2014, 02:20

... a fold that has been observed for all family 1 glycosyl hydrolases. The sequence and structure of the b-glucosidase from P. fu- riosus resemble those of the b-glucosidase of Sulfolo- bus solfataricus. ... Tris ⁄ DCl buffer (pD 8.0) (all other conformational measurements), leading to final pD values of 6.4 and 7.5, respectively, and final protein contents of 100 and 50 mgÆmL )1 , res...

Ngày tải lên: 07/03/2014, 03:20