báo cáo khoa học: " Genome-wide analysis of eukaryote thaumatinlike proteins (TLPs) with an emphasis on poplar" doc

... Peumans WJ, Menu-Bouaouiche L, Van Damme EJM, May GD, Fernandez Herrera A, Van Leuven F, Rougé P: Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit. Planta ... cidic cleft domain is strongly conserved among eukaryote TLPs. Results Annotation, phylogeny, genomic distribution and gene expression of poplar TLPs In contrast to Tuskan and c...

Ngày tải lên: 11/08/2014, 11:21

... gels were scanned u sing an Epson Perfection 1200S colour scanner from Seiko Epson Corporation ( Nagano, Japan). Skeletal muscle proteomics Excision of protein spots, trypsin digestion, and protein identification ... speeds and longer centrifugation periods can fractionate the muscle homogenate into relatively d istinct fractions. However, both cross-contamination of vesicular membrane p...

Ngày tải lên: 19/02/2014, 16:20

... the pattern of splicing of E1A mRNA in comparison with empty pEGFP vector (Fig. 4B). Expression of EGFP– Ki-1 ⁄ 57 leads to formation of the 10S and 9S iso- forms, concomitantly with a reduction of 13S ... the recombinant EGFP-fused form of Ki-1 ⁄ 57 in our con- focal analysis, mainly because of the insufficient qual- ity of the images obtained by labeling the endogenous K...

Ngày tải lên: 16/03/2014, 02:20

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Ngày tải lên: 07/08/2014, 17:22

... formation process, and an optimized amount of 1,8-octanedithiol can be determined. As the amount of 1,8-octanedithiol was increased, the i ntensity of the UV-Vis absorption and the crystallinity of ... to strong interchain interactions. When adding 1,8-octane- dithiol, the absorption band of P3HT:PCBM c omposite film peaks are red-shifted, and the intensity of the absorption ban...

Ngày tải lên: 20/06/2014, 23:20

... Perfection V750 Pro transmis- sion scanner (Epson, Nagano, Japan) and analyzed with imagemaster 2d platinum software, version 5.0 (GE Healthcare). Spots were detected automatically by the soft- ware ... reveals quantitative changes in 23 proteins Proteomic investigations were conducted on b-gal and a-syn cells treated or not with dopamine, as described above. Statistical analysis, by...

Ngày tải lên: 15/02/2014, 01:20

... consequence of cirrhosis, and on consumption of food containing tyramine. Tyramine poisoning causes head- ache, hypertensive crisis and adverse interactions with antidepressive ... hydrolysis of o-NAC and o-NPA. The observation that benzalkonium is an inhibitor of BuChE-catalysed hydrolysis of ATMA at low [S] and an activator at high [S] suggests that the conforma- tional ch...

Ngày tải lên: 18/02/2014, 17:20

... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... type of nonlinear regression shows that the method can be used as an excellent approximation of FI traces and to quantitatively esti- mate the contribution of each phas...

Ngày tải lên: 19/02/2014, 05:20

... Titration of another cationic porphyrin, H 2 TMPyP, yielded qualitatively similar absorption spectra and difference spectra in the Soret band region (not shown). Analysis of association constants and ... absorption and difference absorption spectra Absorption spectra of CuTCPP (a tetra-anionic porphyrin) in the Soret band region in the absence and presence of different concen tratio...

Ngày tải lên: 19/02/2014, 16:20

... DEC1 mutants w as con- firmed b y Western blot analysis, a lthough the expression levels of DEC1 mutants varied among clones. To examine the dose-dependency of t ranscriptional suppression by DEC1, ... were con firmed by W estern b lot analysis with anti-DEC1 and anti-VP16 immunoglobulin (lower p anels). Faint, non- specific bands were also observed, even when the probe was i ncubated...

Ngày tải lên: 19/02/2014, 16:20