báo cáo khoa học: " Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L ) Millspaugh]" pptx

... report of development and validation of a comprehensive set of genic-SSR markers in pigeon- pea b y deep transcriptome sequencing using next gen- eration sequencing technology. A set of 2,877 genic-SSR markers ... genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L. ) Millspaugh] Sutapa Dutta 1,2 , Giriraj...

Ngày tải lên: 11/08/2014, 11:21

... increase in intracellular Ca 2+ levels are involved in the action of neokyotorphin in L929 fibroblasts. Protein kinases involved in the action of neokyotorphin To study the involvement of non-MAPK ... Ca 2+ channels, protein kinase A (PKA), Ca 2+ ⁄ calmodulin-dependent kin- ase II (CaMK II) and mitogen-activated protein kinas- es (MAPK ⁄ ERK) in the effect of neokyotorphin....

Ngày tải lên: 07/03/2014, 11:20

... length (a) 24 (0.15% of X) Number of targeted DNRs having a minimum of 6 repeats (b) 46 (0.29% of X) Number of non-targeted DNRs having a minimum of 6 repeats (c) 10 (0.06% of X) Total number of DNRs ... sequenced after screening 446 (2.83% of X) Number of good quality sequences obtained 199 (1.27% of X) Total number of SSR containing clones (Y) 76 (0.48% of X) Numbe...

Ngày tải lên: 12/08/2014, 05:20

... aliquot of the transgene was introduced into E14.1 ESCs by electro- poration. ESCs expressing the transgene were selected by incubation for 7 days in medium containing a concentra- tion of G418 (Invitrogen) ... proliferating cells obtained from each organ maintained expression of the endothelial-specific markers CD31 (B) and Lyve-1 (C). (D) 1,1’-dioctadecyl-3,3,3’,3’-tetramethyli...

Ngày tải lên: 18/02/2014, 17:20

... strings in each order-class one at a time, starting at the end of a word and working toward its beginning. No more than one match is allowed within a single order-class, by DEVELOPMENT OF ... list of endings (concatenations of suffixes) was compiled in the following way: A preliminary list was based on endings found in a small portion of the augmented catalogue bei...

Ngày tải lên: 16/03/2014, 19:20

... this 1 . In addition, the Indonesian linguists seem to be keen on working “manually” instead of using computers in conducting their linguistics researches as stated in Muhadjir (199 5), only few of ... emergence of Internet as a global information repository, where information of all kind is stored, requires intelligent information processing tools (i.e., computer applications...

Ngày tải lên: 17/03/2014, 07:20

... XML technology. The constraints are used in two mo- des: checking the validity of a document regard- ing a set of constraints; supporting the linguist in his/her work during the building of a corpus. The first ... (Simov et. al., 200 1). The main aim behind the design of the system is the minimization of human intervention during the creation of language resources. It incor...

Ngày tải lên: 24/03/2014, 03:20

... hK1, chymotrypsin (Chtr), plasma kallikrein (PK), urokinase (uPA) and human neutrophile elastase (HNE) enzymes. The Table 1. Alignment of RSL (reactive serpin loop) of recombinant serpin a1-antichymotrypsin ... intercept). Fig. 3. Formation of complex between hK2 and recombinant inhibitors. (A) hK2 was incubated 3 h at 37 °CwithrACT 8.20 (lane 1), rACT 6.2 (lane 2), rACT 8.3 (lane...

Ngày tải lên: 30/03/2014, 13:20

... 5¢-CCGCGTCGACGGAAAAGCGCCTCCCCTAC-3 ) [32]. Recombinant plasmid pLLTK was obtained by insert- ing all three of the amplified fragments into the multiple cloning sites of pcDNA3.1( +) ⁄ zeo (Invitrogen, Carlsbad, CA, USA) by cohesive-blunt ... trans- genic mice of TK5-F1 (including three #), TK5-F2 (including two # and one $) and TK3 aged 8–14 weeks, received tail vein injec...

Ngày tải lên: 30/03/2014, 16:20

... measuring protein– protein interaction. The same insect cell line can be routinely used to express any recombinant proteins of interest, allowing various combinations of molecules to be tested in a ... same insect cell line can be used routinely for expressing any recombinant proteins of interest, allowing various combinations of molecules to be tested in a rapid fashion for prote...

Ngày tải lên: 30/03/2014, 20:20