... ACMSD of the region surrounding the metal active site and the Arg47 likely involved in substrate binding. Discussion In this study, the expression of the human ACMSD transcript coding for the active ... in the brain of a murine model of the syndrome [18,19]. Interestingly, picolinate is reportedly able to prevent the neurotoxic effects of quinolinate in...
Ngày tải lên: 19/02/2014, 02:20
... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma- tional ... activity The enzymatic activity of the recombinant protein was examined in FLS incubations employing dihydroquercetin or dihydrokaempferol as a substrate. Both these fl...
Ngày tải lên: 21/02/2014, 03:20
báo cáo khoa học: " XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells" docx
... somatostatin and Octreoti de-induced expression of XAF1 mRNA and protein in LNCaP cell line. Cells were stimulated with 1 nM somatostatin (a and b) and 1 nM Octreotide (c and d) for the time periods indicated. ... 29:162 http://www.jeccr.com/content/29/1/162 Page 4 of 8 Figure 4 Time-dependent somatostatin and Octreotide-induced expression of XAF1 mRNA and prote...
Ngày tải lên: 10/08/2014, 10:20
Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vector motion. In Fig. 4, most of the points ... J(0) and J(x N ) as a function of s , using a simple Lorentzian function. The left-hand inset shows an overview of the theoretical curve and the fitti...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... connects the a5 and a6 helices in the pRN1 prim–pol protein. Because of a two-residue gap in the corresponding region of the Rep245 sequence, this finding had...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... proteins including isoforms ⁄ degraded protein products and around 65 of these are IgE-binding proteins [19]. Only a few of these proteins have been purified and characterized so far. In the present ... the left of the figure indicate the positions of the deduced amino acid sequence. N-terminal segment determined by protein sequencing is underlined. (B) Comparison o...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... isoforms of the endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse. J. Biol. Chem. 268, 10927–10931. 10. Olsen, S.R. & Uhler, M.D. (1991) Inhibition of protein kinase-a ... & Zhao, S. (2000) Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhib...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... ubiquitin-like (UbL) domain, the XPC-binding domain, and two ubiquitin-associated domains (UBA1 and UBA2) [9,10]. The UbL domain has a high bind- ing affinity for polyubiquitin binding site 2 of the human ... amino acids 566–608 and 613–661 of the hHR23B-binding domain of XPC). However, we not able to detect binding of either XPCB–hHR23B or the complete hHR23B protein...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot
... 396-amino acid long protein, repre- senting the A. gambiae 3-HKT. Expression profile of the 3-HKT gene The expression of the Ag-hkt gene was analysed by RT-PCR amplification of the corresponding ... understand- ing of the XA metabolism in the mosquito and offers a new possible target for the development of insecticides and ⁄ or transmission blocking agen...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx
... cooperativity in the binding of arogenate, paralleling those findings for the interaction of prephenate with chorismate mutase-prephenate dehydrogenase [35]. NADP increases the apparent affinity of the plant ... that of phenylalanine. Indeed, the monofunctional plants arogenate dehydrogenase and E. coli chorismate mutase-prephenate dehydrogenase are at the branch point...
Ngày tải lên: 17/03/2014, 11:20