báo cáo khoa học: " Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing" doc

... corresponding target genes and study of their roles will elucidate possi-ble functions of miRNAs and target genes in relevantprocesses of seed development.Figure 8 GO analysis of targets of known and ... sequencing. Methods 2007, 43:110-117.doi:10.1186/1471-2229-11-5Cite this article as: Song et al.: Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing. ... gma-miR1530 targets may also participate in root development.Targets of new miRNAs from soybean In addition to identification of the targets for known miRNAs (Table 3) , targets of new miRNAs wereinvestigated...

Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx

... kDa), c-globulin (158 kDa), ovalbumin(44 kDa), myoglobin (17 kDa) and vitaminB12 (1.35 kDa) standards. (Lower) Fractionscontaining standards and analyzed proteins of (1) GST–mSPS1 and (2) His–mDLP1, ... synthases in mice and humans, we can ask which component is more import-ant in determining the chain length of ubiquinone by replacing either component with homologs from otherspecies and analyzing ... of pSTVK28. pSTVhDLP1 was digested withBamHI and HindIII and cloned into the same sites of pQE31 to yield pQhDLP1, which was in turn digested withXhoI and HindIII and cloned into the SalI and...

Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx

... wasreplaced by medium with 0.5 lgÆmL)1doxycycline. After12 h of induction, cells were starved in 2 mL of methio-nine ⁄ cysteine-free DMEM for 1 h, and pulsed for 90 minwith 1 mL of medium containing ... upstream of the KDEL-type tetra-peptide contributes to the interaction with the receptor[29]. Moreover, mutagenesis of Asp50 did not affectbinding of DDEL-containing ligands in vitro and in vivo, ... substitute for KDEL in medi-ating lysozyme retention in COS cells. On the otherhand, in vitro experiments have shown quite efﬁcientbinding of an HDEL tetrapeptide and even weak bind-ing of a DDEL tetrapeptide...

Báo cáo khoa học: "Outcomes of resection and non-resection strategies in management of patients with advanced colorectal cancer" doc

... situation and showed successful palliation in 90% of 336 reported caseswith technical failure reported in 8% of cases and perfora-tion in 4% [12]. In this series the technique of stentingwas introduced ... confirmed by 71% of elective surgery patientshaving an ASA status of I or II and a median age of 72. Theelective operative mortality of 14% is high but given thesmall number of patients in this ... optimise QoL and survival taking into account the side effects and risks of any active intervention. In patients that present requiringemergency surgery, due to perforation or bleeding, thedecision...

Báo cáo khoa học: "Identification of a novel germ-line mutation in the TP53 gene in a Mexican family with Li-Fraumeni syndrome" doc

... analysis of p53 mRNA and pro-tein in this family in order to further elucidate the conse-quences of the mutation in the expression of p53 and thepossible mechanism of carcinogenesis in carriers of ... has been reported recently of at least nineisoforms of TP53, generated either by alternative splicingor by the presence of an internal promoter within intron4 (none of which resembled the putative ... reported asinvolved in several other alterations, including deletions and insertions of one or several nucleotides. In 1994,Birch et al [3] reported a complex mutation involvingdeletion of 11 base...

Báo cáo khoa học: "Density of CD4(+) and CD8(+) T lymphocytes in biopsy samples can be a predictor of pathological response to chemoradiotherapy (CRT) for rectal cancer" ppt

... RC, we retrospectively examined the density of tumor infiltrating CD4(+) and CD8(+) T cells using immunohistochemical staining of biopsy samples before CRT, and examined the correlationwith tumor ... find-ing is in line with the data in this study, and suggestedthat the maintenance of circulating lymphocytes numbercan recruit many anti-tumor lymphocytes into the irra-diated tumor during ... was funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Ministry of Health, Labor and Welfare of Japan.Authors’ contributionsJK participated in the study...

báo cáo khoa học: "Dysregulation of CXCL9 and reduced tumor growth in Egr-1 deficient mice" doc

... hypothesized that it wasbeing made in situ by some type of invading host-derivedWeight of tumors grown in wild type and knockout miceFigure 1Weight of tumors grown in wild type and knockout mice. ... Marra F, Vanini V, Maggi E,Romagnani S: Interferon-inducible protein 10, monokineinduced by interferon gamma, and interferon-inducible T-cell alpha chemoattractant are produced by thymic epithe-lial ... tumors after 12 days of growth in knockout mice and performed immunofluorescencestaining using antibodies against Mig. We found punctatestaining that colocalized with expression of CD68, whichis...

báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx

... et al., Inhibition of angiogenesis- and inflam-mation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP Journal of Nanobio-technology ... anti-angiogenesis and anti-inflammation agents in a human colon cancer HCT116 cell line in vitro, and in induced angiogenesis in ovo.Results: The studies described in this report indicate that ... pancreas and leukemias and appears to controlmany cellular processes [10]. Because of their roles in angiogenesis, carcinogenesis, and apoptosis, VEGF and COX-2 are excellent targets for developing...

báo cáo khoa học: "Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection" docx

... Lorenz (Göttingen) for continuous supportwith microalgal handling, database work and interpretation of data, Prof. Dr.Rüdiger Hardeland (Göttingen) for providing with dinophyte strains. Thiswork ... strains, missing only in the Haptophyta, Dinophyta and Euglenoids (Table 3). It was most frequently detected in two lineages of green al gae, the prasinophytes and theStreptophyta. In prasinophytes, ... i.e. it was detected in 17 out of 41examined genera. GLA distribution was rather variablewithin strains and species of a certain streptophytegenus, similar to findings of ARA in other genera....

báo cáo khoa học: " Dissection of genetic and environmental factors involved in tomato organoleptic quality" doc

... dissection of flavour traitsto investigate the role of single genes as well as a genenetwork. For instance, silencing genes of interest canallow identification of key genes or regulatory elements in ... 8 of 10the PC2 factor determined the location of Sarno eco-types in the lower left-hand part and those of Sorrento in lower right-hand part of the graphic. Instead, thePCA plots o btained by ... expression of genes involved in sugar and acid biosynthesis should be designed. In the secondcase, candidate genes should be identified and trans-ferred into breeding lines. The emerging information...

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