báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx

... Lee et al.: Isolation and functional characterization of CE1 binding proteins. BMC Plant Biology 2010 10:277. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... proteins CEBFs (CE1 binding factors). DNA -binding and transcriptional activities of CEBFs Binding of a number of CEBFs, which were isolated as multiple isolates...

Ngày tải lên: 11/08/2014, 11:21

... vector constructs used for functional analysis of the Ppbec1, Ppxero2 and Pptha1 promoter-uidA fusions. LB and RB: left and right T-DNA border. (B) Histochemical GUS staining of fruit slices from ... this injury. A better understanding of cold acclimation and cold- responsive genes in peach trees and fruits may provide clues about the association of cold acclimation and...

Ngày tải lên: 12/08/2014, 03:21

... expression, and thus can be a valuable tool in understanding carotenoid metabolism in tomato. Methods Isolation and cloning of ShCYC-B promoter Isolation of 5' flanking region of CYC-B gene ... gene in S. lycopersicum and S. habrochaites The mRNA levels of CYC-B gene in leaf and flower, and at different stages of fruit development of S. lycopersicum cv. Pusa Ru...

Ngày tải lên: 12/08/2014, 03:21

... that of tobacco and tomato HQT. Example of comparison between absorption spectrum of the reaction product and authentic standardFigure 5 Example of comparison between absorption spectrum of the ... analysis; AR and RL performed the HPLC's. All authors read and approved the final man- uscript. Acknowledgements We thank Prof. G. Mauromicale and Dr. R. Mauro of the Uni...

Ngày tải lên: 12/08/2014, 05:20

... presented here reveals functional simi- larities and differences between Iba1 and Iba2. We investigated Ca 2+ binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin binding and cross-linking ... protein composed mainly of a helices (Figs 1 and 2). The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands 1 and 2, each consisting of two a heli...

Ngày tải lên: 16/03/2014, 06:20

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... FEBS 3585 characterization and evaluation of HYD Js from Jannaschia sp. CCS1. Characterization of HYD Js To explore the possible cause of the higher specific activity for c...

Ngày tải lên: 18/02/2014, 08:20

... describes the cloning and functional characterization of an AK gene from a genomic library of L. donovani as well as its expression and molecular characterization. Materials and methods Materials Plasmids ... 29, 24 and 12 h at temperatures of 4, 13 and 26.5 °C, respectively. Inhibition of AK by the specific inhibitor Ap5A and its effect on growth of L. donovani pro...

Ngày tải lên: 21/02/2014, 00:20

... Pisarchik and A. Slominski (Eur. J. Biochem. 271) Ó FEBS 2004 Molecular and functional characterization of novel CRFR1 isoforms from the skin Alexander Pisarchik and Andrzej Slominski Department of ... with internal deletions and unusual isoforms composed of soluble extracellular (ligand -binding) domains [18]. As the skin shows poly- morphism in CRFR1 expression and its...

Ngày tải lên: 07/03/2014, 15:20

... transcript. Expression and biochemical characterization of VBARP The predicted sizes of the proteins encoded by VBARP-L and VBARP-S are 69 and 49 kDa, respec- tively. To test this, C-terminal V5-tagged VBARP-L and ... distribution of VBARP is independent of the cycling stage of the cells. Identification of biological function(s) of VBARP using a siRNA assay To examine t...

Ngày tải lên: 07/03/2014, 21:20

... chemi- cals and reagents were of analytical grade. Isolation and purification of lamjapin from kelp The fresh tender leaves of kelp were freeze-dried and powdered in liquid nitrogen. Thirty grams of the ... mimics the S/R domain of rat ribosomal RNA (SRD RNA) is an useful substrate for studying the mechanism of action of RIP and for analysis of the chemistry of reco...

Ngày tải lên: 08/03/2014, 10:20