báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot
báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot
... 11:167http://www.biomedcentral.com/1471-2229/11/167Page 6 of 14RESEARCH ARTICLE Open AccessEST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomiWaheed Mahomed1,2 and ... 5:45-56.doi:10.1186/1471-2229-11-167Cite this article as: Mahomed and van den Berg: EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi. BMC Plant ... using 454 pyrosequencing and profiled nine defence-related genes from Pc -infected avocado roots.Results: 2.0 Mb of data was generated consisting of ~10,000 reads on a single lane of the GS FLX...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... The influence of Mg2+on the kinetic properties of APase from E. coli. The influence of Mg2+on the kinetic properties of APase from E. coli inthepresenceofaZn2+to dimer ratio of 2 : 1 in 2A2M1Pbuffer, ... structure and the reaction mechanism of APase from E. coli, suggested by Kim and Wyckoff [1], aswell as the high resolution crystal structure determined byStec et al. [10], offers clarification of ... form of a dianion [9],however, the slow dissociation of Pi,andtheslowphosphorylation of Ser102 by Pi, are both in accordance with Pibinding in form of a trianion.The crystal structure of...
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Báo cáo khoa học: " The Antinociceptive and Anti-inflammatory Effect of Ethylacetate Extracts from Bang-Poong (Radix ledebouriellae) on the Freund’s Adjuvant-Induced Arthritis in Rats" pot
... effect with just a few adverse effects in human. To find effectivecomponent of BP on inflammation, ethylacetate fraction of BP (BPE) and hexane fraction of BP (BPH) were extracted from whole BP and ... curative effect of BPE and BPH, wepreliminarily examined the suppressive effect of thesefractions in arthritic rats with various doses of BPE and BPH (data not shown). As a consequence of preliminarystudy, ... chanical, thermal pain threshold and analysis of spinal Fos expression) and anti- in-flammatory (evaluation of paw edema, seruminterleukin-6 level and x-ray analysis) effect of BPextracts w ere examined....
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" ppt
... targets of miR-10b, miR-412 or miR-494.Additional file 10: Figure S6 - Ingenuity Pathway Analysis™™ of thepotential target genes of miR-494. Twelve of the mRNA target genes of miR-494 from Table ... associated with C3(1)/Tag tumors and nine potential target genes (Table3). The expression of four miR-412 probes was alsoassociated with basal-like tumors (Table 4) and four pre-dicted target genes, ... hierarchical clustering analysis of miRNA gene expression of 41 ma mmary tumors derived from 8 geneticallyengineered mouse models and samples of 5 normal mammary glands from 17.5-day-pregnant FVB/N...
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" docx
... targets of miR-10b, miR-412 or miR-494.Additional file 10: Figure S6 - Ingenuity Pathway Analysis™™ of thepotential target genes of miR-494. Twelve of the mRNA target genes of miR-494 from Table ... associated with C3(1)/Tag tumors and nine potential target genes (Table3). The expression of four miR-412 probes was alsoassociated with basal-like tumors (Table 4) and four pre-dicted target genes, ... as potential miRNA target genes by this a nalysis. For instance, the expression of only 19out of 156 TargetScan predicted targets were inverselycorrelated with the expression of miR-10b, and...
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Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf
... mechanismdifferent from the heat-induced activation of hsp70promoter, and the region between )206 and )187 bp of the hsp70 promoter is necessary for Hsp105b-induced expression of the hsp70 gene. STAT3, ... of Hsp105b was necessary but not suffi-cient for the Hsp105b-induced expression of the hsp70 gene. The promoter region between )206 and )187 bp of the hsp70 gene is essential for activation of the ... )206 and )187 bp of the hsp70 promoter and enhanced the expression of the hsp70 gene in the cells overexpressingHsp105b, STAT family proteins seem to play animportant role in the regulation of...
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Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt
... forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and probed with the ... function of the putative lysosomal SC Scpep1,we analysed the molecular properties of Scpep1 and generated an Scpep1 gene trap (Scpep1-gt) mousemodel.ResultsMolecular forms of Scpep1In order to generate ... transferase) and urine parameters showed no pathological findings.The activities of several lysosomal hydrolases werenormal in various tissues and lysosomes from liver and kidney of Scpep1-gt mice, and...
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Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx
... in the induction of SREBP-1a gene expression. When endogenous Sp1 expression was reduced to 20% of normal with gene- specific small interfering RNA (siRNA), the gene expression of SREBP-1a was ... activities of SREBP tar-gets. HepG2 cells (A and B) and Caco-2 cells (C) were transfected with 200 ng of the reporter constructs consisting of the indicated gene promoters and 200 ng of pEF-b-Gal. ... involvement of the O-glycosylation of Sp1 in the induction of SREBP-1a transcription byglutamine. The gene expression of SREBP-1c, whichrepresented less than 20% of the SREBP-1a gene expression...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... (lane 6); and purified recombinantBmKIM (lane 7).Ó FEBS 2002 Molecular cloning and function of gene BmKIM (Eur. J. Biochem. 269) 4471Molecular cloning and functional expression of a gene encoding ... kinase.Screening of the venom gland cDNA libraryTen thousand clones from the venom gland cDNA librarywere analyzed by the32P-end-labelled oligonucleotideprobe. High and low density screenings of bacterial ... toxins from BmK (BmKIM, BmKAEP and BmKIT2), but Arg6 or Lys6 and Thr57 are conserved inother scorpion species.Cloning and analysis of genomic sequence of BmKIMWe have isolated, cloned and sequenced...
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báo cáo khoa học: " Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1" potx
... developed from crosses between resistant (Salinas and Salinas 88) and susceptible (Valmaine) cultivars. Sequencing of thesemarkers from a set of 68 cultivars revealed a relatively high level of nucleotide ... Salinas × UC96US12 map. Moreover, Tvr1is one of the few resistance genes that was not at a geneticposition coincident with any type of candidate resistance gene so far mapped in lettuce [52]. Thus, ... HDA-GT12DNA analyzer and scored by Biocalculator software (both from eGene, Irvine, CA). If sequencing was needed, PCRproducts were first treated with Exonuclease I and subse-quently with Antarctic...
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