báo cáo khoa học: " Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening" potx

... from linkage group 2 interacting with the second QTL from linkage group 14. F. one QTL from linkage group 6 interacting with the second QTL from linkage group 12. In each case, the peak of the ... position within a marker interval can be estimated by treating the position is fixed . Using a grid search, we can obtain the MLE of t he QTL posi- tion from the peak of the...

Ngày tải lên: 11/08/2014, 11:21

... 137-140. doi:10.1186/1471-2229-11-149 Cite this article as: Fortes et al.: Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening. BMC Plant Biology 2011 11:149. Submit ... 34 RESEARCH ARTICLE Open Access Transcript and metabolite analysis in Trincadeira cultivar reveals novel...

Ngày tải lên: 11/08/2014, 11:21

... pro- tein synthesis was measured by incubating cells at 37 °C for 90 min in the presence of 1 lCi [ 35 S]-methionine in NaCl ⁄ P i . The ID 50 values obtained in the absence (–) or (+) presence of ... previously (Experimental Procedures and the legend to Fig. 6), following 48 h exposure to serial dilutions of the toxins and measuring the remain- ing protein synthes...

Ngày tải lên: 23/03/2014, 15:21

... Stem+Morph+DOMAIN, and Stem+Morph+UNIQUE. Below, we only report the best-performing results across the N-GRAM fea- tures and their combinations. In each case, our base- line is the majority class in the ... run the typical 5 fold cross validation where we train on 4 folds and test on the 5th and then average the re- sults. In the test setting, we only ran with t...

Ngày tải lên: 20/02/2014, 05:20

... 1 c-Actin Actin, cytoplasmic 1 Tubulin b Tubulin a Ankyrin repeat domain-containing protein 18A Ankyrin repeat domain-containing protein 18A Heat-shock protein b1 a-Actin-2 Unclassified Keratin, ... phos- phorylation-dependent 14-3-3-binding protein in con- trol conditions. Analysis using the highest-stringency settings in the scansite algorithm showed Ser39 in vimentin to be the...

Ngày tải lên: 06/03/2014, 22:21

... is no example of mutational analysis of all of these residues in the same enzyme. To obtain an insight into how the many conserved residues in the active sites of family 18 chitinases contribute ... affinity of ChiB for allosamidin, which is an analogue of the proposed oxazolinium ion intermediate [32]. The minor (or even positive) effects of Y214F on substrate a...

Ngày tải lên: 07/03/2014, 14:20

... hydrolysis of the nucleotide. To investi- gate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites ... do interact in vivo. We assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for their inte...

Ngày tải lên: 07/03/2014, 16:20

... enzymes involved in the thiamin biosynthesis in H. pylori, and the peculiar environment in which it thrives in, leads to the hypothesis of the existence of a reduced thiamin biosynthetic pathway. Indeed, ... Tyr. The hypothesized mechanism for the reaction of B. subtilis TenA [17] assumes that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favo...

Ngày tải lên: 16/03/2014, 00:20

... in the substrate binding sites of these structures instead of the water molecule present in the native structure, these ÔblobsÕ could not be interpreted (data not shown). Mutagenic analysis of ... the other hand, in Fe-type NHase, the side-chain of the corresponding serine residue does not interact with the valine residue. aTyr114 is also located near the cyste...

Ngày tải lên: 16/03/2014, 16:20

... buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of retinol caused an increase of the emission of the single Trp residue, ... result in quenching of protein Trp fluorescence and in an increase of the specific DAUDA emission after the noncovalent incorporation of the ligand. Binding...

Ngày tải lên: 16/03/2014, 18:20