báo cáo khoa học: "Characterization of a caleosin expressed during olive (Olea europaea L ) pollen ontogeny" pdf

... of caleosin in the olive pollen and locular fluid. Sections from olive anthers at the mid microspore (A) , young pollen grain (B), mid bicellular pollen grain (C) and mature pollen grain (D) stages ... from olive anthers at the pollen mother cell (PMC), tetrad (Te), microspore (Mi), young pollen grain (YP), mid bicellular pollen grain (MBP) and mature pollen grain (M...

Ngày tải lên: 11/08/2014, 11:21

... for absorption by the cell, whereas intracellular peptidases play a major role in regulation of metabolism [2]. The families of chymo(trypsin) (S 1) and subtilisin (S 8) are regarded as the largest ... peptidases are clearly indicated by the fact that 2% of all genes encode peptidases (or their homologues) in all kinds of organisms [1]. Extracellular peptidases hydrolyse large...

Ngày tải lên: 19/02/2014, 07:20

... Western blot analysis of overexpressed D. melanogaster DPP III. (A) D. melanogaster DPP III partially purified from cytosol of transfected S 2 cells (1 lg) was electrophoresed on a 7.5% (w/v) acrylamide ... Polyvar) and image analysis software (Spot RT, Diagnostic Instru- ments, USA). Partial purification of a putative D. melanogaster DPP III expressed in S 2 cells Typically, 50 mL...

Ngày tải lên: 17/03/2014, 10:20

... Sallantin, M., Huet, J C., Demarteau, C. & Pernollet, J C. (199 0) Reassessment of commercially available molecular weight stan- dards for peptide sodium dodecyl sulfate-polyacrylamide gel ... Angeli, S., Marchese, S., Brandazza, A. , Ferrara, L. , Pelosi, P. & Scaloni, A. (200 1) Bacterial expression and conformational analysis of a chemosensory protein from Schistocer...

Ngày tải lên: 21/02/2014, 03:20

... kDa), aldolase (158 kDa), conalbumin (75 kDa) and albumin (43 kDa) (GE Healthcare HMW Gel Filtration Standards; GE Healthcare, Milwaukee, WI, USA). In addi- tion, P. putida BFDC was included as ... tryptophan catabolism [2]. It may also be involved in tyrosine metabolism but, when alternative pathways are avail- able, it is unlikely to play any significant role in the catabolism of the branc...

Ngày tải lên: 06/03/2014, 00:21

... CLAP_1:AA GATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1888 CLAP_2:AAGATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (A) 48 ... CLAP_1:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT 1300 CLAP_2:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATT...

Ngày tải lên: 07/03/2014, 16:20

... differentially expressed. One of the polypeptides migrated with an apparent molecular mass of 14.4 kDa and a pI value of  5.8 (Fig. 1, spot 1 5) and was only found in cells cultured at high (0.8 lm) ... Methylococcus capsulatus (Bath) Odd A. Karlsen, Linda Ramsevik, Live J. Bruseth, Øivind Larsen*, Annette Brenner, Frode S. Berven, Harald B. Jensen and Johan R. Lillehaug Departm...

Ngày tải lên: 07/03/2014, 17:20

... the available databases, it was recently established by NMR studies and molecular modeling that PA1b belongs to the cystine-knot family [8]. Characterized only recently as a structural family ... the microsomal fraction of S. oryzae extract was found to be saturable and reversible, with an a nity (K d )of2 .6n M ,and a high maximal binding capacity (B max )of4 0pmolÆmg )1 of pro...

Ngày tải lên: 08/03/2014, 02:21

... substrate concentrations. SEM values were calculated by fitting data by a weighted linear regression using the software SigmaPlotÒ. AlabNA, L- alanine-b-naphthylamide; AlapNA, L- alanine-p-nitroanilide; ... molitor [22], soluble Tineola bisselliela (Lepido- ptera) aminopeptidase [35,36], soluble Attagenus megatoma (Coleoptera) aminopeptidase [37], soluble and microvillar R. americana (Dipt...

Ngày tải lên: 16/03/2014, 12:20

... the abnormal accumulation of intracellular acyl-CoA. We have isolated a thioesterase from Alcaligenes faecalis ISH108 and demonstrated its application in chemoselective and racemization free deacylation ... 3B. Intracellular localization of thioesterase We carried out electron microscopic immunogold labe- ling studies with ultrathin sections of Alcaligenes cells to localize thioestera...

Ngày tải lên: 16/03/2014, 13:20