báo cáo khoa học: "A single amino acid change within the R2

báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx

... 11:117 http://www.biomedcentral.com/1471-2229/11/117 Page 11 of 14 RESEARC H ARTIC LE Open Access A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters ... (R69L) located in the R2 domain of VvMYB5b and predicted to affect the formation of a sa...

Ngày tải lên : 11/08/2014, 11:21

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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

... Comparison of the three pri- mary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas. Derivation of the amino acid sequence of ovine DNase and revision of the previously ... anesthesia with diethyl ether. Preparation of the samples for the assays and determination of enzyme activity were performed as described previously [7,21...

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo y học: "A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5α" potx

... model of the capsid protein To obtain further insight into the effects of the G-to-E single amino acid substitution at the 116th position of the CA on its three-dimensional (3-D) structure, the ... Structural model of the N-terminal domain of HIV-1 CA with G116E substitution. Panel A shows the template structure of the N-terminal domain of the H...

Ngày tải lên : 13/08/2014, 01:20

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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... clearly demonstrate that the conformation of CD2 is unchanged. After the insertion of the helix–loop–helix EF-hand domain from STIM1, the helical content of the engineered protein CD2.STIM1.EF increased, ... are sensed by the canonical EF-hand motif and cause conformational changes in this motif. The Ca 2+ signal change and the accompa- nying conformational ch...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

... half-reaction involving the conversion of FAD (ﬂavin cofactor) to FADH 2 and concomitant oxidation of the amino acid to an imino acid. The imino acid intermediate of the oxidation pathway undergoes nonenzymatic ... 390 and 475 nm, 375 and 450 nm, and 370 and 450 nm, respectively. The spectral features of the dissociated cofactor were similar to those of...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot

... obviates the need of looping of intervening DNA. The in vivo data supporting the MutS–MutL foci formation suggests the possibility of extensive recruitment of protein molecules at the sites of mismatch ... analyses presented here offers an equilibrium study of the com- plex changes brought about by MutS ATP hydrolysis and mismatch binding. Therefore the current...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khóa học: A single mutation that causes phosphatidylglycerol deﬁciency impairs synthesis of photosystem II cores in Chlamydomonas reinhardtii pdf

... synthesis. In the absence of D1, the rate of synthesis of apoCP47 is strongly reduced, while the rates of synthesis of both D1 and apoCP47 drops when D1 cannot assemble within PSII complexes, for ... reinhardtii,thefattyacid C16:1(3t) could increase the efﬁciency of PG for PSII core biogenesis in the situation of active synthesis determined by the high growth...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

... three less than that of the B. subtilis enzyme. In the former enzyme the corresponding amino acids were cysteine, alanine and serine. Therefore, it is possible that the thermophilic enzyme is ... central intermediate in the de novo and salvage biosynthesis of pyrimidine, purine and pyridine nucleotides as well as in the biosynthesis of the amino acids histidine...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus pptx

... °C for 10 min; 35 cycles of 95 °C for 1 min, 48 °C for 1 min and 72 °C for 1 min; 72 °C for 9 min. The PCR products were subcloned into the T-vector and sequenced. The nucleotide sequence of the ... ‘+’. The asterisk indicates the speciﬁc band of the antibacterial protein. (C) 2D gel electrophore- sis shows a single spot of antibacterial protein indica...

Ngày tải lên : 29/03/2014, 08:20

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Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx

... both protein variants, the rate of reduction of the FMN cofactor was very small, amounting to 0.6% and 3% of the wild-type rate for the D137L and K109L ⁄ D137L protein variants, respectively. The ... against each other by 90° and packing of the two dimers. To test the hypothesis that tetramerization is responsible for increased thermosta- bility, the appa...

Ngày tải lên : 30/03/2014, 01:20

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