... with 0.5% SDS and 0.5 mgÆmL)1proteinase K for 15 min at 65 °C and subsequently for 1 hat 37 °C for DNA analysis, and were analyzed by 1% ag-arose gel electrophoresis followed by staining with ... and Kuin the aggregates is noteworthy because they functionin a common pathway, and the protein kinase activityof DNA-PKcs is greatly stimulated by Ku duringassembly at DNA ends [30]. We examined ... that is disrupted by phosphorylation. Biochemistry 41, 12706–12714.34 Sarkaria JN, Tibbetts RS, Busby EC, Kennedy AP,Hill DE & Abraham RT (1998) Inhibition of phos-phoinositide 3-kinase...