Báo cáo y học: "Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model" pdf
Báo cáo y học: "Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model" pdf
... were again washed and resuspended in 1 ml saline and subjected to flowcytometry. Cells col-lected using flow cytometry on a FACS were analyzed using FlowJo software by gating on the lymphocyte popu-lation ... this article as: Kashyap et al., Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model Journal of Immune Based Therapies and Vaccines 2010, 8:3 ... aim of the present study is to investigate the immune responses to repeated stimulation of PBMC& apos;s from BCG vaccinated healthy volunteers with BCG vaccine. Materials and methodsStudy SubjectsParticipants...
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Báo cáo y học: "Assessment of knowledge about childhood autism among paediatric and psychiatric nurses in Ebonyi state, Nigeria" doc
... was found to be higher in domain 3 (symptoms of obsessive and repeated pattern of behaviour), followed by do main 1 (symptoms of impairments in social interaction), domain 4 (type of dis-order ... first-year and fourth-year stu-dents with respect to their knowledge of various aspects of autism, including diagnosis, cause, symptomatology,treatment and outcome. Fourth-year students were onlysignificantly ... dis-orders, years of experience as a health worker and area of specialty with workers in psychiatric facility scoringhigher than those working in paediatric settings.Another survey showed that...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... used to assess the toxicity of RGD-LCL, we observed cytotoxicity of li-posomes beginning at the 1.25 mg/mL or 2.5 mg/mL concentrations. Indicating that the liposomes them-selves have some cytotoxic ... the use of this material could augment the effects of matrine on cancer cells in vitro. Key words: Matrine, Liposomes, Cyclic arginine- glycine-aspartic acid, Drug delivery systems. Introduction ... freeze-drying, was separated by mini-gel columns of Sephadex-G50 prepared in advance. In brief, incubation mixtures of 200μL containing unloaded matrine and RGD-M-LCL were added onto mini-gel...
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot
... cell of the anterior fat body, AFP immunostaining isrestricted to the cytoplasm. (C) AFP immunostaining can also be found in the cytoplasm of haemocytes. (D) In situ hybridization of longitudinalcryosections ... body by two-hybrid assays. In addition to theconversant interactors arylphorin and LSP-2, which belong to the hexamerin family, we identified AFP as a stronginteractor. T he two-hybrid analysis ... LSP-2),confirming the ability of the experimental system to identifyproteins that interact with the hexamerin receptor. Thirteen of the library plasmids contained cDNAs that encodednonhexamerin interactors....
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Báo cáo y học: "Effect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosus" potx
... JF:Detection of autoantibodies to Sm antigen in systemic lupuserythematosus by immunodiffusion, ELISA and immunoblot-ting: variability of incidence related to assays and ethnic origin of patients. ... C, Klein R, Burmester GR,Schoessler W, Hiepe F: Casein is an essential cofactor in autoantibody reactivity directed against the C-terminal SmD1peptide AA 83–119 in systemic lupus erythematosus. ... polypeptides have recentlybeen shown to contain sDMA, and this constitutes a majorautoepitope within the C-terminus of SmD1 and SmD3[14,18,37]. In one of these studies, a synthetic peptide of SmD1...
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Báo cáo y học: "Association of phospholipase A2 receptor 1 polymorphisms with idiopathic membranous nephropathy in Chinese patients in Taiwan" pptx
... quantitativedetermination of total urine protein. The M-TP reagentwas used to measure protein concerntration by a timedendpoint method. Protein in the sample reacted with the pyrogallol red (PR) molybdate ... significantly higher in patients with IMN ascompared with controls (p = 0.005). In addition, haplotypes of PLA2R1 may be used to predict the risk of IMN(p = 0.004). Haplotype H1 plays a role in an increased ... rs35771982 may increase the incidence of IMN.Haplotype analysis of PLA2R1All the haplotypes presented in our study are shown in Table 2. Comparisons of the haplotype frequenciesbetween patients with...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx
... that polymorphonuclea r leukocyte splay essential roles in the development of inflammatoryresponses via the production of several types of chemi-cal mediators and inflammatory cytokines [5]. ... inhibition of inflammatory cytokine produc-tion and polymorphonuclear leukocyte activation, mayaccount for the clinical effectiveness of macrolides in inflammatory airway diseases [1,3-6,8]. Recently, ... resulting in prevention of tissue injury in patients with chronic air-way diseases, including CS.The cellular response to LPS is transmitted from thecell membrane to the cytoplasm through the Toll-likeLPS...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx
... leukocyte splay essential roles in the development of inflammatoryresponses via the production of several types of chemi-cal mediators and inflammatory cytokines [5]. Reactiveoxygen species ... josamycin [2]. There is considerable evidence to suggest that the anti-inflammatory action of macrolides,such as the inhibition of inflammatory cytokine produc-tion and polymorphonuclear leukocyte ... roxithro-mycin and azithromycin into m ice once a day for 4weeks significantly suppress NO generation induced byLPS injection [28]. Pretreatment of mice with telithro-mycin, one of ketolide antibiotics...
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Báo cáo y học: "Effects of dietary phytoestrogens on plasma testosterone and triiodothyronine (T3) levels in male goat kids" pdf
... Messina M, Gardner C, Barnes S: Gaining insight into the healtheffects of soy but a long way still to go: commentary on thefourth International Symposium on the Role of Soy in Pre-venting ... 2008,199(3):351-365.23. Antony FF, Aruldhas MM, Udhayakumar RC, Maran RR, Govindara-julu P: Inhibition of Leydig cell activity in vivo and in vitro in hypothyroid rats. J Endocrinol 1995, 144(2):293-300.24. ... statistically significant.Conclusion: Our findings suggest that phytoestrogens can stimulate testosterone synthesis duringpuberty in male goats by increasing the secretion of T3; a hormone known to...
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Báo cáo y học: " Evolution of the HIV-1 envelope glycoproteins with a disulfide bond between gp120 and gp41" pdf
... chains into the proteininterior, but the glutamine side chains may rearrange to form hydrogen bonds to regain some of the lost energy.Similar to Gln-Gln-Gln interactions that are present in ... orientation of their side chains differs (fig. 6). The side chain of residue593 (cyan in fig. 6) points towards the interior of the loop,thereby establishing an interaction with its counterparts in ... replicated with a delay of approxi-mately 4 days compared to the wt virus. Replication of theQ591L mutant was significantly better, with a delay of only one day compared to the wt virus. Of note...
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