Báo cáo khoa hoc:" The Mayer-Rokitansky-Küster-Hauser syndrome (congenital absence of uterus and vagina) – phenotypic manifestations and genetic approaches" docx
Báo cáo khoa học: "The interaction between different types of activated RAW 264.7 cells and macrophage inflammatory protein-1 alpha" potx
... on top of the wells. MF suspensions (200 μL) were placed on the top of wells and the cham- ber was incubated at 37ºC for 120 min. The filters were removed and nonmigrating cells (facing the top ... in the process of RPI [27] , but they could not completely explain its pathogenesis. The important roles of macrophages at different stages of RPI and the interactio...
Ngày tải lên: 09/08/2014, 09:20
Báo cáo khoa học: The Cockayne syndrome group B protein is a functional dimer docx
... and the seven conserved DNA-dependent ATPase motifs (I, IA and II to VI). The five CSB fragments cover amino acids 2–3 41, 31 0–5 20, 46 5–1 056, 95 3–1 204, and 118 7–1 493 of CSB, respectively. (B) The ... 430 6–4 314 ª 2005 FEBS glutaraldehyde of the CSB dimer showed that CSB exists as a dimer in solution and indicated that the dimer forms in the absence...
Ngày tải lên: 23/03/2014, 15:20
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... residues 1–5 05 and 65 3–7 25 of TAP1 and residues 49 4–6 39 of TAP2 and chimera 2C1 encoded residues 1–4 93 and 64 0–7 03 of TAP2 and residues 50 6–6 52 of TAP1. The exchange was performed at the amino ... in the TAP variants 1–2 C1 (D) and 2–1 V2 (E). From our data we propose that the defect in the ADP to ATP exchange in the chimeric NBD1 of...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Purification and properties docx
... amounts of purified FDH1 used for isolation of the pterin cofactor were not sufficient to allow quantification or the identification of the exact nature of the molybdopterin cofactor. However, the primary ... 0.2 UÆmg )1 . About half of this specific activity was found in extracts of cells grown in the medium containing molybdate and no tungstate. In the absence of...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... (Vps4p–TRP). Thus, the roles of the conserved sequences at the start and end of the C-terminal helix are distinct. Addition of Vps4p–E233Q and ⁄ or Vta1p did not stimulate the ATPase activity of the ... not the b domain. The distinguishing feature of members of the meiotic clade of AAA ATPases is the SRH motif, which dif- fers from that of other AAA ATP...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain ppt
... complete loss of the ability of the protein to phosphorylate light-activated rhodopsin. Interestingly, gradual removal of the C-terminal-most 16 residues of mGRK6-A M1 affected the activity of the protein toward ... func- tional significance of the differences in their primary structures. Whereas mGRK6-A, -B and -C are similar in terms of their overall structural or...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf
... (D1a–K9, K14a– D14–R4–E8 and E13–R14d). Besides the covalent disulfide connection between Cys1 and Cys122, seven salt bridges, grouped into five clusters (D1a–R206, E8–K202–E14c, D14–R137, K135–E14e–K186d ... Eqns ( 2–4 ) involved in the hydrolysis of the synthetic substrate D-Phe-Pip-Arg-pNA (B) at 25 °C in the presence of 0.15 M NaCl. The best-fit pK a values of the ion...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids doc
... genome. These enzymes occur independently of the ecotype and the genome size (Fig. S4). They are encoded in the 7 Mb genome of Mycobacterium smegmatis str. MC2 155, in the reduced 4.4 Mb genome of the ... mycobacteria. Quantification of the products from different substrates indicates that the preference for each of the cleavage positions is determined by the hydr...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: The proximity between C-termini of dimeric vacuolar H+-pyrophosphatase determined using atomic force microscopy and a gold nanoparticle technique ppt
... Table 1. The AFM image of reconstituted V-PPase shows a ratio of approximately 2.40 : 1 for the height values of the cytosolic and luminal sides. In addition, the theoreti- cal ratio of the total ... (peak 2) represented those of the GNPs bound to the C-termini of the enzyme (Fig. 6C). The height of the latter protrusions (4.9 ± 0.1 nm) reflects the sum o...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc
... only PEX1 and PEX6 are AAA- ATPases. Dysfunction of the ATPase activity of either of the two proteins results in peroxisome biogenesis disorders [ 4–7 ]. The ATPase activities of PEX1 and PEX6 are ... in vivo [22]. On the other hand, colocalization of VCP and PtdIns-4-kinase IIa on the buoyant subfraction of ER-derived mem- brane was observed [66]. The ND of...
Ngày tải lên: 16/03/2014, 12:20