Báo cáo khoa hoc:" Folic acid modified gelatine coated quant

Báo cáo khoa hoc:" Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics" pdf

... 7 RESEARCH Open Access Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics Valérie A Gérard 1 , Ciaran M Maguire 1 , Despina Bazou 2 and Yurii ... potential for in vitro cancer diagnostics. Keywords: Quantum Dots, Folic acid, cancer, bio-imaging Background Nanoparticles and especially quantum do...

Ngày tải lên : 11/08/2014, 08:20

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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf

... defined at 95°C for 5 min, followed by 35 cycles of denaturing at 95°C for 45s, anneali ng at 59°C for 35 s and extension at 72°C for 1 min, and followed by the final extension at 72°C for 10 min. ... prediction of metastasis as well as recurrence of cervical cancer. DNMT methyltransferase 1 (DN MT1), which plays a significant role in maintaining DNA methylation status and re...

Ngày tải lên : 10/08/2014, 10:21

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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... pyrophosphate, followed by rinsing with water, before being dried under infrared lamps. Scintillation counting was performed using Rotiszint (Roth, Karlsruhe, Germany). Aminoacylation Aminoacylation was performed ... guanidinium g-N but also comes into contact with the adenosine ring of Ade76. There is a general consensus that tRNA binding is not required for arginine binding [33], although...

Ngày tải lên : 18/02/2014, 13:20

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Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot

... Tobacco transcription factor TGA2.2 is the main component of as- 1- binding factor ASF-1 and is involved in salicylic acid- and auxin- inducible expression of as- 1-containing target promoters. J. Biol. Chem. ... which contains an authentic as- 1 element as found in the CaMV 35S RNA promoter (in bold letters). The as- 1 element was inserted at position )591 in the PR-1a promoter...

Ngày tải lên : 23/03/2014, 15:21

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Báo cáo khoa học: " Retinoic acid inducible gene I Activates innate antiviral response against human parainfluenza virus type 3" pdf

... which infected cells activated innate response following HPIV3 infection was not known. In the current study, we demonstrated that RIGI serves as a PRR in HPIV3 infected cells to induce innate ... activation of innate antiviral response during HPIV3 infection. Moreover, RIGI was involved in activating both arms (NFκB/TNFα and IRF3/IFNα/β) of innate immunity following HPIV3 infection....

Ngày tải lên : 12/08/2014, 04:20

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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... 3A). An increase in ARF content was observed in endosomal fractions isolated 5 min postinjection of CT-B, and this increase was maintained for up to 60 min (Fig. 3A, upper panel). CT administration ... H + -ATPase pump and in vitro vacuolar acidiﬁcation in hepatoma HepG2 cells. The vacuolar H + - ATPase inhibitor baﬁlomycin and the cathepsin D inhibitor pepstatin A partially inhib...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

... interactor. Nucleolin was ﬁrst described as a major nuclear protein consisting of a negatively charged N-terminal domain, an RNA-binding domain and a C-terminal domain rich in RGG motifs [54]. Nucleolin has ... carried out solid-phase binding assays using A B C D E Fig. 1. Afﬁnity chromatography isolation of protein tyrosine phosphatase-r (PTPr)-binding and lactoferrin-binding protein...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx

... radioactivity incorporated into the nucleic acids was measured after TCA precipitation and plotted against the incubation time in minutes. T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus ... of heparin as the trapping molecule. As shown in Fig. 6A when the wild-type (–)IRES was used as template for HCV NS5B in the presence of heparin, a template sized RNA was...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo khoa học: Alternative splicing: regulation of HIV-1 multiplication as a target for therapeutic action docx

... transcrip- tase or of the protease involved in virus protein matura- tion. The absence of cellular proteins resembling HIV integrase has also been exploited to develop novel anti- HIV molecules that inhibit ... exploited in strategies aimed at modifying the expression level of a given SR protein or hnRNP protein. The one relying on RNA interference appears particularly interesting. Indeed...

Ngày tải lên : 06/03/2014, 09:22

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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot

... Protein A Agarose ⁄ Salmon Sperm DNA incubation for no more than 1 h. Beads were washed twice for 15 min in lysis buffer containing 150 m m NaCl, followed by a 15-min wash in lysis buffer containing 500 ... 2009) doi:10.1111/j.1742-4658.2009.06979.x Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI ⁄ SNF chromatin remodel...

Ngày tải lên : 07/03/2014, 00:20

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