Báo cáo khoa hoc:" Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics" pdf

... 7 RESEARCH Open Access Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics Valérie A Gérard 1 , Ciaran M Maguire 1 , Despina Bazou 2 and Yurii ... potential for in vitro cancer diagnostics. Keywords: Quantum Dots, Folic acid, cancer, bio-imaging Background Nanoparticles and especially quantum do...

Ngày tải lên: 11/08/2014, 08:20

... defined at 95°C for 5 min, followed by 35 cycles of denaturing at 95°C for 45s, anneali ng at 59°C for 35 s and extension at 72°C for 1 min, and followed by the final extension at 72°C for 10 min. ... prediction of metastasis as well as recurrence of cervical cancer. DNMT methyltransferase 1 (DN MT1), which plays a significant role in maintaining DNA methylation status and re...

Ngày tải lên: 10/08/2014, 10:21

... pyrophosphate, followed by rinsing with water, before being dried under infrared lamps. Scintillation counting was performed using Rotiszint (Roth, Karlsruhe, Germany). Aminoacylation Aminoacylation was performed ... guanidinium g-N but also comes into contact with the adenosine ring of Ade76. There is a general consensus that tRNA binding is not required for arginine binding [33], although...

Ngày tải lên: 18/02/2014, 13:20

... Tobacco transcription factor TGA2.2 is the main component of as- 1- binding factor ASF-1 and is involved in salicylic acid- and auxin- inducible expression of as- 1-containing target promoters. J. Biol. Chem. ... which contains an authentic as- 1 element as found in the CaMV 35S RNA promoter (in bold letters). The as- 1 element was inserted at position )591 in the PR-1a promoter...

Ngày tải lên: 23/03/2014, 15:21

... which infected cells activated innate response following HPIV3 infection was not known. In the current study, we demonstrated that RIGI serves as a PRR in HPIV3 infected cells to induce innate ... activation of innate antiviral response during HPIV3 infection. Moreover, RIGI was involved in activating both arms (NFκB/TNFα and IRF3/IFNα/β) of innate immunity following HPIV3 infection....

Ngày tải lên: 12/08/2014, 04:20

... 3A). An increase in ARF content was observed in endosomal fractions isolated 5 min postinjection of CT-B, and this increase was maintained for up to 60 min (Fig. 3A, upper panel). CT administration ... H + -ATPase pump and in vitro vacuolar acidification in hepatoma HepG2 cells. The vacuolar H + - ATPase inhibitor bafilomycin and the cathepsin D inhibitor pepstatin A partially inhib...

Ngày tải lên: 19/02/2014, 02:20

... interactor. Nucleolin was first described as a major nuclear pro- tein consisting of a negatively charged N-terminal domain, an RNA-binding domain and a C-terminal domain rich in RGG motifs [54]. Nucleolin has ... carried out solid-phase binding assays using A B C D E Fig. 1. Affinity chromatography isolation of protein tyrosine phosphatase-r (PTPr)-binding and lactoferrin-binding protein...

Ngày tải lên: 19/02/2014, 05:20

... radioactivity incorporated into the nucleic acids was measured after TCA precipitation and plotted against the incubation time in minutes. T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus ... of heparin as the trapping molecule. As shown in Fig. 6A when the wild-type (–)IRES was used as template for HCV NS5B in the presence of heparin, a template sized RNA was...

Ngày tải lên: 20/02/2014, 01:20

... transcrip- tase or of the protease involved in virus protein matura- tion. The absence of cellular proteins resembling HIV integrase has also been exploited to develop novel anti- HIV molecules that inhibit ... exploited in strategies aimed at modifying the expression level of a given SR protein or hnRNP protein. The one relying on RNA interference appears particularly interesting. Indeed...

Ngày tải lên: 06/03/2014, 09:22

... Protein A Agarose ⁄ Salmon Sperm DNA incubation for no more than 1 h. Beads were washed twice for 15 min in lysis buffer containing 150 m m NaCl, followed by a 15-min wash in lysis buffer containing 500 ... 2009) doi:10.1111/j.1742-4658.2009.06979.x Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI ⁄ SNF chromatin remodel...

Ngày tải lên: 07/03/2014, 00:20