... denaturant–binding interactions; histidine-phosphocarrier; protein stability. A full understanding of the physical interactions underlying the structure, folding and the function of a protein requires a ... borne in mind that the most probable origin of the discrepancies is the experimental uncertainty in determining the third derivative of the stability curve (m DC pi ), ob...
Ngày tải lên: 19/02/2014, 12:20
... thermophilic proteins contain more arginines on the protein surface than mesophilic proteins [36–38]. The effect of the large-scale increase in the number of arginines was tested experimentally in T. reesei ... in bleaching of pulp to reduce the use of toxic chlorine- containing chemicals [1] or to improve the quality of animal feed [2]. In addition, there are applications...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... substitution of 11 serine and threonine residues with g lycine and alanine, which would increase the peptide hydrophobicity. The minor amounts of other peptides observed in the chromatographic profiles in ... proximity to the protein surface is fundamental to protein f olding, stability, recognition and activity, and thus understanding protein hydration is crucial in unrave...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... of insoluble substrates. Bound protein was determined indirectly by measuring the concentration of free protein in the supernatants after 24 h of incubation. L. lactis chitinase and chitin-binding ... carbohydrate- binding proteins and domains from T. fusca [26]. Genes encoding family 33 CBPs occur even in bacte- ria containing otherwise seemingly simple chitinolytic machineri...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... distinct functions in cells. STAT3 is mostly involved in cell survival and proliferation, and STAT1 is involved in anti-viral and immune defence and cell death, in response to interfer- ons, including ... lg of nuclear protein extracts were incubated for 30 min at 4 °C in binding buffer (1% NP40, 50 mm Hepes, pH 7.6, 140 mm NaCl) containing salmon sperm DNA (1 lg per ass...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
... 24 h in the absence or presence of the general inhibitor of transcription actinomycin D, and changes in the actin cytoskeleton were monitored by fluorescence using rhodamine phalloidin staining. ... and titrated in HEK293 cells as described previously [22]. Adenoviral transient infections of cells using multiplicity of infection leading to a maximal rate of infection efficie...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: "Low-cost Enrichment of Spanish WordNet with Automatically Translated Glosses: Combining General and Specialized Models" pdf
... Translation Models In this section we study the possibility of combin- ing out -of- domain and in- domain translation mod- els aiming at achieving a good balance between precision and recall that yields ... second baseline system (‘WNG’) is entirely based on the training set from of the in- domain corpus of parallel glosses. In the second case phrase pairs occurring only once...
Ngày tải lên: 23/03/2014, 18:20
Báo cáo khoa học: Engineering thermal stability of L-asparaginase by in vitro directed evolution ppt
... purposes. This incompatibility often relates to the stability of the enzymes under body conditions. In the case of engineering proteins for thermostability, researchers are in the enviable position of being ... glutamate dehydrogenase and sample containing l-ASNase activity. Alternatively, the rate of ammonia formation was measured Engineering thermal stability of L-as...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein pot
... metal-binding domains of ATP7A) protein–protein interaction between MNK2 and HAH1 is unlikely. Given that the surface charges of MNK2 and WND2 are fairly similar, a preferential interaction of the ... surface of Atx1 and HAH1 is quite similar, but that of the metal binding domains of Ccc2 is somewhat different from ATP7A ⁄ ATP7B [11]. In addition, although the two metal-bi...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo khoa học nông nghiệp " Improvement of Vietnamese vegetable production using GAP principles for seed and seedling production and superior hybrid varieties " MS3 doc
... was of a high standard, even for international seedling production. His knowledge and skills and record keeping, and those of his senior staff (including his wife) were of a very high standard, ... training in tomato and/ or cucumber production. They displayed good knowledge of the principles and practices of IPM and the major tomato and cucumber pests and diseas...
Ngày tải lên: 21/06/2014, 04:20