... Westerholm-Parvinen1, Nisse Kalkkinen2,Johanna Buchert1 and Kristiina Kruus11 VTT Technical Research Centre of Finland, Espoo, Finland2 Protein Chemistry Research Group and Core Facility, Institute ... reactions in themultistep biosynthesis of melanin pigments, beingresponsible, for instance, for skin and hair pigmenta-tion [4]. Tyrosinases play an important role in regula-tion of the oxidation–reduction ... performed using aQ-TOF instrument (Micromass Ltd, Manchester, UK).Protein and peptide sequencingN-terminal protein and peptide sequencing was performedusing a Procise 494A Sequencer (Perkin Elmer,...