báo cáo khoa học: " Engineering of an E coli outer membrane protein FhuA with increased channel diameter" doc

... Access Engineering of an E. coli outer membrane protein FhuA with increased channel diameter Manuel Krewinkel, Tamara Dworeck and Marco Fioroni * Abstract Background: Channel proteins like FhuA can be an alternative ... between channel sur- face area in crease and kinetic results can be either due to an increase in channel diameter or to an unlikely increa...

Ngày tải lên: 11/08/2014, 00:23

... the question whether the FhuA Δ1-159 Ext really acts as a channel or whether the observed kinetics are due to the locally perturbed polymer membrane by the presence of the protein, the channel was ... to ensure the insertion of the FhuA Δ1-159 Ext into the E. coli membrane. An increased hydrophobic mismatch between the protein and the lipid membrane would forbid the...

Ngày tải lên: 11/08/2014, 00:23

... further advantage of the T7 system lies in i ts better tolerance with respect to temperature changes: lowering the reaction temperature from 37 to 30 °C decreased protein yields insignificantly, ... tandem phage k p R and p L promoters, when the reactions were supplemented with purified phage T7 or E. coli RNA polymerase. Nineteen samples, each selectively labelled with a different...

Ngày tải lên: 16/03/2014, 18:20

... mm.We used MALDI-TOF MS combined with PNGase F (New England Biolabs, Herts, UK) treatment to identify its N-glycan structures and their sites of expression. Protein sequence and degenerate primed ... growing colonies of B. cinerea were placed in PDA plates covered with sterile cellophane sheets. Mycelia were allowed to grow for 48 h and then plugs from growing colonies of Trichode...

Ngày tải lên: 19/02/2014, 16:20

... luciferin ⁄ luciferase assay kit, and expressed relative to the values in untreated control cells. Values are means ± SD from three independent experiments expressed relat- ive to control taken ... helicases, and RNA binding increases the ATPase activity of these proteins [26]. To test whether the ATPase activity of IRP-1 is also enhanced in the presence of IRE, we measured hydrolysis...

Ngày tải lên: 07/03/2014, 09:20

... performed with nRNE-d, lanes 3 and 4 represen t EMSA performed with nuclear extracts prepared 8 h after partial he patectomy an d lan es 5 and 6 represent EMSA performed with nuclear extracts prepared ... described earlier [ 19]. Fraction nRNE-d and rRNE-d were separated on 12% SDS/PAGE and electrophoretically transferred o nto a nitrocellulose membrane. The membrane was t hen incu...

Ngày tải lên: 16/03/2014, 18:20

... sites yielded poor expression levels as well (Table 2, seven and nine sites off) due to retention of the protein inside the cell, as shown by Western blotting. As the expression level of these ... bundle ( helices 383±372 and 526±543; human Table 2. I n¯uence of the number and position of N-glycosylation sites on the expression level of secreted human BChE. The presence or absence...

Ngày tải lên: 17/03/2014, 11:20

... of the F195S mutant, fluorescence emission spectra and fluorescence intensi- ties were measured in the presence of different concen- trations of AdoMet. The F195S mutant protein showed significantly ... adenine residues – one in the upper strand and one in the lower strand [24].Yet another variation is seen in the case of MmeI, where it has been reported that MmeI modifies the adenine i...

Ngày tải lên: 22/03/2014, 21:20

... preference. • The result is the candidate with the maximum occurrence probability. To show the iuformation mentioned above, candi- dates are expressed by the tree in Figure 1. This tree is an example ... low the root node needs not to be decided because they are leaf nodes. When more than two ambiguous words exist in an input sentence, a node may often have one that is not a le...

Ngày tải lên: 23/03/2014, 19:20

... and NNC-FKBP22. A His-tag attached to the N-termini of the proteins is represented by a shaded box. The a-helices and b-strands are represented by cylinders and arrows, respectively. These secondary structures ... close proximity to the Met8 of the other monomer. Both residues are located close to the bottom of the cleft of the V-shaped structure. Therefore, it is strongly expected th...

Ngày tải lên: 30/03/2014, 01:20